Draft Genome Sequences of Two Clinical Mastitis-Associated Escherichia coli Strains, of Sequence Type 101 and Novel Sequence Type 13054, Isolated from Dairy Cows in Bangladesh

Rahman, Mohammad Hafizur; Zowalaty, Mohamed E. El; Falgenhauer, Linda; Khan, Md. Mukhlesur Rahman; Alam, Jahangir; Popy, N; Rahman, Bahanur

doi:10.1128/mra.00166-23

Cited by 2 publications

(4 citation statements)

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“…Milk samples were obtained from 36-month and 46-month-old female lactating Holstein Friesian ( Bos taurus taurus ) cows as previously reported 13 . Cow udders were washed with clean water and dried, then the udder teats were rubbed with 70% ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…Cow udders were washed with clean water and dried, then the udder teats were rubbed with 70% ethanol. The first two strings were discarded, and California mastitis test was performed to determine the milk somatic cell counts in milk samples as previously reported 13 . Milk samples (10 mL) were collected in sterile tubes and samples were transported to the laboratory maintaining a cold chain for further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Milk samples (500 µL) were inoculated in 10 mL nutrient broth and incubated at 37°C for 18 hours and subsequently streaked on mannitol salt agar media (HiMedia). The inoculated plates were incubated at 37 °C for 24 hours and sub-cultured to isolate presumptively identified S. aureus pure colonies as previously reported 13 .…”

Section: Methodsmentioning

confidence: 99%

“…S. aureus PCR-confirmed isolates were submitted to Invent Technology Ltd. (Banani, Dhaka, Bangladesh) for whole-genome sequencing as recently reported 13 . Sequencing libraries were prepared using a Nextera XT library preparation kit (Illumina Inc., CA, USA) and sequencing was performed on the Illumina NextSeq 550 platform (Illumina Inc., CA, USA) using the high-output reagent kit with 150 nt maximal read length.…”

Section: Methodsmentioning

confidence: 99%

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Molecular identification and whole genome sequence analyses of methicillin-resistant and mastitis-associated Staphylococcus aureus sequence types 6 and 2454 isolated from dairy cows

Rahman,

El Zowalaty,

Falgenhauer

et al. 2024

J. Genomics

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The emergence of antimicrobial-resistant and mastitis-associated Staphylococcus aureus is of great concern due to the huge economic losses worldwide. Here, we report draft genome sequences of two Staphylococcus aureus strains which were isolated from raw milk samples obtained from mastitis-infected cows in Bangladesh. The strains were isolated and identified using conventional microbiological and molecular polymerase chain reaction (PCR) methods. Antibiotic susceptibility testing was performed. Genomic DNA of the two strains was extracted and the strains were sequenced using the Illumina NextSeq 550 platform. The assembled contigs were analyzed for virulence determinants, antimicrobial resistance genes, extra-chromosomal plasmids, and multi-locus sequence type (MLST). The genomes of the two strains were compared with other publicly available genome sequences of Staphylococcus aureus strains. The raw read sequences were downloaded and all sequence files were analyzed identically to generate core genome phylogenetic trees. The genome of BR-MHR281strain did not harbour any antibiotic resistance determinants, however BR-MHR220 strain harbored mecA and blaZ genes. Analysis of BR-MHR220 strain revealed that it was assigned to sequence type (ST-6), clonal complex (CC) 5 and spa type t304, while BR-MHR281 strain belonged to ST-2454, CC8, and harbored the spa type t7867. The findings of the present study and the genome sequences of BR-MHR220 and BR-MHR281 strains will provide data on the detection and genomic analysis and characterization of mastitis-associated Staphylococcus aureus in Bangladesh. In addition, the findings of the present study will serve as reference genomes for future molecular epidemiological studies and will provide significant data which help understand the prevalence, pathogenesis and antimicrobial resistance of mastitis-associated Staphylococcus aureus .

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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