Draft genomic sequence of a chromate- and sulfate-reducing Alishewanella strain with the ability to bioremediate Cr and Cd contamination

Abstract

Alishewanella sp. WH16-1 (= CCTCC M201507) is a facultative anaerobic, motile, Gram-negative, rod-shaped bacterium isolated from soil of a copper and iron mine. This strain efficiently reduces chromate (Cr6+) to the much less toxic Cr3+. In addition, it reduces sulfate (SO42−) to S2−. The S2− could react with Cd2+ to generate precipitated CdS. Thus, strain WH16-1 shows a great potential to bioremediate Cr and Cd contaimination. Here we describe the features of this organism, together with the draft genome and comparative genomic results among strain WH16-1 and other Alishewanella strains. The genome comprises 3,488,867 bp, 50.4 % G + C content, 3,132 protein-coding genes and 80 RNA genes. Both putative chromate- and sulfate-reducing genes are identified.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-016-0169-3) contains supplementary material, which is available to authorized users.

The Cytochrome bd Complex Is Essential for Chromate and Sulfide Resistance and Is Regulated by a GbsR-Type Regulator, CydE, in Alishewanella Sp. WH16-1

“…Cloning of genes neighboring the Tn 5 transposon was performed according to the plasmid rescue method described before ( Chen F. et al, 2015 ). The resulting neighboring sequences were searched against the whole genome of strain WH16-1 ( Xia et al, 2016 ) using the NCBI BLAST server.…”

“…They are widely distributed and play a key role in the environment ( Muyzer and Stams, 2008 ; Barton and Fauque, 2009 ). Various SRB have exhibited great potential for environmental bioremediation applications, such as participating in the precipitation of heavy metals to produce metal sulfides ( Xia et al, 2016 ; Zhou et al, 2016 ), reduction of toxic metals ( Barton and Fauque, 2009 ), degradation of azo dyes ( Pandey et al, 2007 ), and nitroaromatic compound respiration ( Barton and Fauque, 2009 ).…”

“…Chromate is chemically analogous to sulfate and enters cells mediated by the sulfate ABC transporter CysPUWA in various bacteria ( Viti et al, 2014 ). Some SRB are also chromate-reducing bacteria (CRB) ( Viti et al, 2014 ; Xia et al, 2016 ). Accordingly, Cr(VI) induces the expression of the sulfate transporter and competes with sulfate in some bacteria ( Viti et al, 2014 ).…”

“…WH16-1 (=CCTCC M201507) was isolated from soil of a copper and iron mine. It possesses great potential in metal bioremediation since it reduces sulfate and chromate or generates CdS/PbS precipitation ( Xia et al, 2016 , 2018 ; Zhou et al, 2016 ). Strain WH16-1 also showed a high tolerance to Cr(VI) (MIC of 45 mM) ( Zhou et al, 2016 ).…”

Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery

“…According to the species definition of stablishing the cut-off for new species in 98.7 % of 16S rRNA gene sequence identity (Yarza et al, 2014) The genus Alishewanella was described for the first time by Vogel et al (2000) (Vogel et al, 2000) and emended independently by Roh et al (2009), Kim et al (2010) and Sisinthy et al (2017) (Kim et al, 2010;Roh et al, 2009;Sisinthy et al, 2017;Vogel et al, 2000). It belongs to the family Alteromonadaceae order Alteromonadales of the class Gammaproteobacteria (Xia et al, 2016). According to Sisinthy et al (2017), Alishewanella genus contains a total of 8 species: A. aestuarii, A.…”