Draft Whole-Genome Sequences of Haemophilus influenzae Biogroup aegyptius Strains Isolated from Five Brazilian Purpuric Fever Cases and One Conjunctivitis Case

Abstract

Brazilian purpuric fever is a febrile hemorrhagic pediatric disease caused by Haemophilus influenzae biogroup aegyptius, a bacterium which was formerly associated with only self-limited purulent conjunctivitis. Here, we present draft genomes of strains from five Brazilian purpuric fever cases and one conjunctivitis case.

Closed Complete Annotated Genome Sequences of Five Haemophilus influenzae Biogroup aegyptius Strains

“…Four of the five strains (F1946, F3028, F3043, and F3037) contain an ∼32.5-kb plasmid (GenBank accession number AF447808) associated with Haemophilus influenzae biogroup aegyptius strains (10). Interestingly, our sequence of strain F1946 is ∼28 kb larger than the WGS sequence deposited recently (11). The extra sequences, able to be resolved here due to long-read sequencing, consist of a number of duplicated regions, such as rRNA gene clusters (∼5.5 kb each), the licABC operon involved in lipooligosaccharide (LOS) biosynthesis (∼2.5 kb), and an ∼6-kb region encoding pilin and the haf pilus export machinery.…”

Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012 - 2019 and ancestor strains

“…Genome determination for studying H. influenzae strains associated with invasive diseases had been carried out by our group when Pereira et al determined the whole-genome of Haemophilus influenzae that caused Brazilian purpuric fever in 2019. 5 The expertise of genomic bioinformatics platforms made possible the analysis of invasive H. influenzae isolated in Clinical Hospital of Campinas State University in this study. This hospital health services covers all the metropolitan area of Campinas with around 3.2 million inhabitants and 20 cities.…”

“…Outbreaks at the Clinical Hospital of University of Campinas, São Paulo State – Brazil have shown an increase of invasive strains after vaccination. The use of Illumina platform for bacterial whole-genome construction set up by our group for characterization of Brazilian Pupuric fever Haemophilus strains (Pereira et al 2019 5 ) was used for this purpose. This work aimed to use the Illumina sequencing method for draft-genome to characterize genome structure and in silico virulence factors of 13 invasive strains isolated from blood and cerebrospinal fluid.…”

Analysis of potential virulence genes and competence to transformation in Haemophilus influenzae biotype aegyptius associated with Brazilian Purpuric Fever

“…Genome comparative analysis identified that Hae accessory genome has 21 specific Hae-BPF putative genes ( Strouts et al ., 2012 ; Pereira et al ., 2019 ) and a much richer repertoire of AT adhesins in comparison to another available genome of Haemophilus genus . A total of eight new trimeric autotransporter adhesins (TAAs) was described in Hae (BPF and conjunctivitis isolates) and are present as homologs, termed tabA - for the Hae-BPF alleles, or tahA - for the Hae non-BPF alleles.…”