Drainage of amniotic fluid delays vocal fold separation and induces load-related vocal fold mucosa remodeling

Lungová, Vlasta; Griffin, Kate V.; Lunga, Tadeas; Thibeault, Susan L.

doi:10.1016/j.ydbio.2020.08.003

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…Ion channels of the transient receptor potential (TRP) superfamily are involved in a wide variety of neural signaling processes, most notably in sensory receptor cells, and have been implicated in chemosensation, 11–15 in addition to their mediated role in mechanotransduction 16–22 . While much is to be uncovered, recent progress into laryngeal somatosensation has localized TRP vanilloid (V) channels 1–4 within adult laryngeal epithelium of mice and humans, 13,15 P2X3 type adenosine triphosphate receptors in type III taste bud cells within murine arytenoid tissue, 23 vagal P2RY1 neural receptors innervating the murine larynx, 24 and PIEZO1 mechanoreceptor in murine laryngeal epithelia 25 . TRPV channels have also been hypothesized to act as laryngeal nociceptors associated with pathological conditions, such as inflammatory response, genesis of cough and asthma 14,15 .…”

Section: Introductionmentioning

confidence: 99%

“… 16 , 17 , 18 , 19 , 20 , 21 , 22 While much is to be uncovered, recent progress into laryngeal somatosensation has localized TRP vanilloid (V) channels 1–4 within adult laryngeal epithelium of mice and humans, 13 , 15 P2X3 type adenosine triphosphate receptors in type III taste bud cells within murine arytenoid tissue, 23 vagal P2RY1 neural receptors innervating the murine larynx, 24 and PIEZO1 mechanoreceptor in murine laryngeal epithelia. 25 TRPV channels have also been hypothesized to act as laryngeal nociceptors associated with pathological conditions, such as inflammatory response, genesis of cough and asthma. 14 , 15 While their assumed importance for chemosensation in adult laryngeal mucosa has been established, 13 , 15 investigation into developmental expression of these essential chemoreceptors is warranted given the importance of LCR in the fetus and newborn.…”

Section: Introductionmentioning

confidence: 99%

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Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges

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Tibbetts

Bartley

et al. 2022

Laryngoscope Investig Oto

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Objective: The primary aim of this study was to identify expression of TRPV3 and TRPV4 chemoreceptors across perinatal and adult stages using a murine model with direct comparisons to human laryngeal mucosa. Our secondary aim was to establish novel cell expression patterns of mechanoreceptors PIEZO1 and PIEZO2 in human tissue samples.Study design: In vivo. Methods:We harvested murine laryngeal tissue to localize and describe TRPV3/4 endogenous protein expression patterns via immunofluorescence analyses across two developmental (E16.5, P0) and adult (6 weeks) timepoints. Additionally, we obtained a 60-year-old female larynx including the proximal trachea and esophagus to investigate TRPV3/4 and PIEZO1/2 protein expression patterns via immunofluorescence analyses for comparison to murine adult tissue.Results: Murine TRPV3/4 expression was noted at E16.5 with epithelial cell colocalization to supraglottic regions of the arytenoids, aryepiglottic folds and epiglottis through to birth (P0), extending to the adult timepoint. Human TRPV3/4 protein expression was most evident to epithelium of the arytenoid region, with additional expression of TRPV3 and TRPV4 to proximal esophageal and tracheal epithelium, respectively. Human PIEZO1 expression was selective to differentiated, stratified squamous epithelia of the true vocal fold and esophagus, while PIEZO2 expression exhibited selectivity for intermediate and respiratory epithelia of the false vocal fold, ventricles, subglottis, arytenoid, and trachea. Conclusion:Results exhibited expression of TRPV3/4 chemoreceptors in utero, suggesting their importance during fetal/neonatal stages. TRPV3/4 and PIEZO1/2 were noted to adult murine and human laryngeal epithelium. Data indicates conservation of chemosensory receptors across species given similar regional expression in both the murine and human larynx.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges

Foote

Tibbetts

Bartley

et al. 2022

Laryngoscope Investig Oto

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Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis

Mohad

Lungová

Verheyden

et al. 2021

Developmental Biology

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Sensory Innervation of the Larynx and the Search for Mucosal Mechanoreceptors

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Thibeault

2021

J Speech Lang Hear Res

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Purpose The larynx is a uniquely situated organ, juxtaposed between the gastrointestinal and respiratory tracts, and endures considerable immunological challenges while providing reflexogenic responses via putative mucosal mechanoreceptor afferents. Laryngeal afferents mediate precise monitoring of sensory events by relay to the internal branch of the superior laryngeal nerve (iSLN). Exposure to a variety of stimuli (e.g., mechanical, chemical, thermal) at the mucosa–airway interface has likely evolved a diverse array of specialized sensory afferents for rapid laryngeal control. Accordingly, mucosal mechanoreceptors in demarcated laryngeal territories have been hypothesized as primary sources of sensory input. The purpose of this article is to provide a tutorial on current evidence for laryngeal afferent receptors in mucosa, the role of mechano-gated ion channels within airway epithelia and mechanisms for mechanoreceptors implicated in laryngeal health and disease. Method An overview was conducted on the distribution and identity of iSLN-mediated afferent receptors in the larynx, with specific focus on mechanoreceptors and their functional roles in airway mucosa. Results/Conclusions Laryngeal somatosensation at the cell and molecular level is still largely unexplored. This tutorial consolidates various animal and human researches, with translational emphasis provided for the importance of mucosal mechanoreceptors to normal and abnormal laryngeal function. Information presented in this tutorial has relevance to both clinical and research arenas. Improved understanding of iSLN innervation and corresponding mechanotransduction events will help shed light upon a variety of pathological reflex responses, including persistent cough, dysphonia, and laryngospasm.

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Drainage of amniotic fluid delays vocal fold separation and induces load-related vocal fold mucosa remodeling

Cited by 3 publications

References 34 publications

Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges

Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges

Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis

Sensory Innervation of the Larynx and the Search for Mucosal Mechanoreceptors

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