Dramatic increase in antimicrobial resistance in ESKAPE clinical isolates over the 2010–2020 decade in India

Kharat, Arun S.; Makwana, Nilesh; Nasser, Mahfouz; Gayen, Samarpita; Yadav, Bipin; Kumar, Durgesh; Veeraraghavan, Balaji; Mercier, Corinne

doi:10.1016/j.ijantimicag.2024.107125

Cited by 5 publications

(3 citation statements)

References 83 publications

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“…Antimicrobial resistance is one of the most urgent global concerns, and the spread of multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan-drug-resistant bacteria is occurring at an increasingly alarming speed [14]. The knowledge of antimicrobial peptides from anuran secretions can significantly contribute to the development of new therapeutic agents.…”

Section: Introductionmentioning

confidence: 99%

First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)

Silva,

Barbosa,

Santana

et al. 2024

Chemistry

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The global emergency of antimicrobial resistance has drawn several efforts to evaluate new drug candidates, such as natural defensive biomolecules. Ocellatins are a group of antimicrobial peptides found in anurans of the Leptodactylidae family. This work investigated the presence of antimicrobial peptides in the skin secretion of Leptodactylus vastus from the Brazilian northeast. The secretion was fractionated by RP-HPLC, and the fractions were screened for antibacterial activity. A peptide isolated from the most active fraction was characterized for primary structure and evaluated for antibacterial activity, cytotoxicity to murine melanoma cells (B16-F10), and hemolytic activity. The RP-HPLC profile displayed 26 fractions, with fraction 25 being the most active. One of the two peptides present in this fraction had the primary structure determined, belonging to the group of ocellatins. Since it was not identical to other ocellatins previously reported, it was named ocellatin-VT. This peptide especially inhibited Gram-negative bacteria growth, with the highest activity against Acinetobacter baumannii and Escherichia coli (growth inhibition was higher than 95% at 8 and 16 µM, respectively). Ocellatin-VT was weakly cytotoxic to B16-F10 cells and showed low hemolytic activity. In conclusion, a new ocellatin was isolated from L. vastus skin secretion that was active against non-resistant and multidrug-resistant bacteria.

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Section: Introductionmentioning

confidence: 99%

First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)

Silva,

Barbosa,

Santana

et al. 2024

Chemistry

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“…Silversol already has a history of clinical use for the management of oral health [ 29 ], skin diseases, diabetic ulcers, burns, and wound care/wound disinfection ( ; accessed on 29 March 2024). As S. aureus is one of the most common clinical isolates [ 30 , 31 ], we made an effort to gain insights into Silversol’s antibacterial activity against this particular pathogen.…”

Section: Introductionmentioning

confidence: 99%

Silversol® (a Colloidal Nanosilver Formulation) Inhibits Growth of Antibiotic-Resistant Staphylococcus aureus by Disrupting Its Physiology in Multiple Ways

Thakkar,

Gajera,

Mehta

et al. 2024

Pharmaceutics

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Antibiotic-resistant strains of Staphylococcus aureus are being viewed as a serious threat by various public health agencies. Identifying novel targets in this important pathogen is crucial to the development of new effective antibacterial formulations. We investigated the antibacterial effect of a colloidal nanosilver formulation, Silversol®, against an antibiotic-resistant strain of S. aureus using appropriate in vitro assays. Moreover, we deciphered the molecular mechanisms underlying this formulation’s anti-S. aureus activity using whole transcriptome analysis. Lower concentrations of the test formulation exerted a bacteriostatic effect against this pathogen, and higher concentrations exerted a bactericidal effect. Silversol® at sub-lethal concentration was found to disturb multiple physiological traits of S. aureus such as growth, antibiotic susceptibility, membrane permeability, efflux, protein synthesis and export, biofilm and exopolysaccharide production, etc. Transcriptome data revealed that the genes coding for transcriptional regulators, efflux machinery, transferases, β-lactam resistance, oxidoreductases, metal homeostasis, virulence factors, and arginine biosynthesis are expressed differently under the influence of the test formulation. Genes (argG and argH) involved in arginine biosynthesis emerged among the major targets of Silversol®’s antibacterial activity against S. aureus.

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“…For several decades now, the bacteria or fungi responsible for common or serious infections have developed resistance to all the new antimicrobials on the market [ 3 ]. Enterobacteria , Staphylococcus , Enterococcus, and fungi of the genus Candida are responsible for human infections without pharmacological options for therapy [ 3 , 4 , 5 , 6 , 7 ]. Faced with this situation, the World Health Organization published a list of bacterial species in 2017 [ 2 ], followed by a list of fungi in 2022 [ 8 ], including Candida albicans and Candida glabrata , for which the development of new therapeutic options was considered a priority to control morbidity and mortality rates worldwide.…”

Section: Introductionmentioning

confidence: 99%

Pfaffia paniculata Extract, a Potential Antimicrobial Agent against Candida spp., Pseudomonas aeruginosa, and Streptococcus mutans Biofilms

Miranda,

Ramos,

Attik

et al. 2024

Microorganisms

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The World Health Organization (WHO) has prioritized developing new drugs against specific bacteria and fungi, such as Enterobacteriaceae and Candida spp. While Pfaffia paniculata is commonly called the “cure-everything”, its scientifically proven benefits are limited to anti-inflammatory and antioxidant actions. Therefore, this study aims to determine the spectrum of antimicrobial activity of Pfaffia paniculata and assess its cytotoxicity. Thus, broth microdilution test was conducted according to the CLSI M7-A9 and M27-A3 reference methods. After screening, microbial species with minimum inhibitory concentration (MIC) values were selected for biofilm tests. These tests evaluated biomass using the crystal violet (CV) test, metabolic activity using the MTT assay, and structural analysis via Scanning Electron Microscopy (SEM). Cytotoxicity was evaluated in human gingival fibroblasts (FMM-1). There were reductions of 29.4 and 42.7% in CV and MTT assays for Candida spp. biofilm. S. mutans and P. aeruginosa biofilms showed a decrease of 15.7 and 28.6%, respectively. Cell viability tests indicated 55.1, 56.9, and 65.5% of viability after contact with 1.93, 0.96, and 0.48 mg/mL of the extract, respectively. The P. paniculata extract showed antimicrobial action, displayed MIC values, and antibiofilm action on P. aeruginosa, S. mutans, and C. albicans. The cytotoxicity on the FMM-1 cell line was dose-dependent. Therefore, P. paniculata extract holds significant potential for developing new drugs.

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Dramatic increase in antimicrobial resistance in ESKAPE clinical isolates over the 2010–2020 decade in India

Cited by 5 publications

References 83 publications

First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)

First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)

Silversol® (a Colloidal Nanosilver Formulation) Inhibits Growth of Antibiotic-Resistant Staphylococcus aureus by Disrupting Its Physiology in Multiple Ways

Pfaffia paniculata Extract, a Potential Antimicrobial Agent against Candida spp., Pseudomonas aeruginosa, and Streptococcus mutans Biofilms

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