Dramatically stabilized phosphotriesterase—polymers for nerve agent degradation

Highly efficient immobilization of glycosylated enzymes into polyurethane foams

“…Cobalt(II) chloride was used as a stabilizer for aminoacylase (Bommarius et al, 1992) and as an initiator for the polymerization reaction (Saunders and Frisch, 1962). Under these conditions, foams were formed with an open-pore structure and with a narrow pore size distribution (LeJeune et al, 1997). Variation of the prepolymer, the amount of water, or different surfactants could effect the physical properties of the foam, such as porosity, density, and surface area.…”

“…Covalent attachment in polyurethane foams has been used to immobilize various enzymes; for instance, ␤-Dgalactosidase (Hu et al, 1993), cellulase (Chakrabarti and Storey, 1988), amyloglucosidase , phosphotriesterase (LeJeune et al, 1997), and lipases . In all cases reported, the enzyme loading was in the order of 1 mg protein per gram foam, which is impractically low for many applications.…”

Evaluation of cell surface-displayed protein stability against simulated gastric fluid

“…Enzymes immobilized on solid supports exhibit increased resistance to inactivation by high temperature and pH (Matsumoto and Kondo 2001;Wang et al 2006). Additionally, immobilization reduces the accessibility of the protein on solid supports to proteolytic cleavage (LiJeune et al 1997). Similarly, BGL1 displayed on the yeast cell surface had an increased resistance to pepsin.…”

Nerve Agent Sensing Biopolymer Wipe

“…Surfactant selection varies CO 2 evolution and has significant effect on the porosity, density, and surface properties of resultant polymers. For the synthesis of substrate polymers, substrates are added to the buffer/ surfactant solution instead of pH dye and enzymes (5)(6)(7)(8)(9).…”