Dried Blood Spot in Laboratory: Directions and Prospects

Abstract

Over the past few years, dried blood spot (DBS) technology has become a convenient tool in both qualitative and quantitative biological analysis. DBS technology consists of a membrane carrier (MC) on the surface of which a biomaterial sample becomes absorbed. Modern analytical, immunological or genomic methods can be employed for analysis after drying the sample. DBS has been described as the most appropriate method for biomaterial sampling due to specific associated inherent advantages, including the small volumes of biomaterials required, the absence of a need for special conditions for samples’ storage and transportation, improved stability of analytes and reduced risk of infection resulting from contaminated samples. This review illustrates information on the current state of DBS technology, which can be useful and helpful for biomedical researchers. The prospects of using this technology to assess the metabolomic profile, assessment, diagnosis of communicable diseases are demonstrated.

Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform

“…Blood samples are typically obtained by venipuncture, followed by blood plasma or serum preparation. Alternatively, a simple finger stick and dried blood spotting allows self-collection, minimizing effort and likely increasing participation ( Au - Grüner et al, 2015 ; Malsagova et al, 2020 ; Thevis et al, 2020 ; Vázquez-Morón et al, 2019 ). Samples collected in this manner may be maintained at ambient temperature and can be shipped using mail or courier service without the need for refrigeration.…”

“…Samples collected in this manner may be maintained at ambient temperature and can be shipped using mail or courier service without the need for refrigeration. Dried blood spots (DBS) have been successfully utilized for immunological/serological testing for multiple viral diseases, including hepatitis C, HIV, and COVID-19 ( Karp et al, 2020 ; Malsagova et al, 2020 ; Vázquez-Morón et al, 2019 ).…”

Stability of Plasma Protein Composition in Dried Blood Spot during Storage

“…Membrane carriers DBS provide opportunity for the long-term storage of biological samples and their transportation to analytical laboratories, as well as for creating biobanks [9]. Today, the use of mass spectrometry for DBS analysis is becoming a routine method in biomedical and pharmaceutical laboratories.…”

“…Review of the related literature shows that DBS technology is a widely used and actual method at the preanalytical stages of diagnosis [9]. It is important that the biomaterial on DBS membrane carriers demonstrates resistance to long-term storage and does not require special storage conditions in comparison with samples of cells or blood plasma [21].…”

DBS Assay with LC-MS/MS for the Determination of Idelalisib, A Selective PI3K-δ Inhibitor in Mice Blood and Its Application to a Pharmacokinetic Study

“…Dried blood spot (DBS) methodology is becoming a valuable tool in recent times for the quantitative analysis of various drugs [11][12][13][14][15][16][17][18]. DBS offers several advantages over traditional sampling (plasma/blood/serum) techniques namely reduction of commercial costs for laboratory equipment, convenience in collection, reduction in collection of blood volume, no requirement of a trained phlebotomist, ease of sample handling/storage/shipping, safety in handling, less time in processing and increase in throughput etc.…”