Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures

Lima, Eleonidas Moura; Rissino, Jorge Dores; Guimarães, Adriana Costa; Overal, David James; Khayat, André Salim; Souza, Patrícia Carvalho de; Christofolini, Denise Maria; Smith, Marı́lia de Arruda Cardoso; Burbano, Rommel Rodrı́guez

doi:10.1590/s1516-89132005000500005

Cited by 3 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HEp-2 cell line is derived from laryngeal carcinoma cells of human nasopharyngeal mucosa. Being slow-growing tumors, these cells develop in animal hosts as well as in tissue culture ( 9 ). Propolis effects on these cells have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp‐2) Cells

Búfalo

Candeias

Sforcin

2007

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propolis's cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2) cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro against HEp-2 cells, in a dose- and time-dependent way. Propolis solvent had no effects on morphology and number of viable cells, proving that the cytotoxic effects were exclusively due to propolis components. Since humans have been using propolis for a long time, further assays will provide a better comprehension of propolis's antitumor action.

show abstract

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp‐2) Cells

Búfalo

Candeias

Sforcin

2007

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The number of cells which arrested in the Metaphase were counted and also the total number of cells (1000 cells were counted) [14], [6], then the mitotic index was calculated as follow:…”

Section: Mitotic Indexmentioning

confidence: 99%

CYTOGENETIC TOXICITY EFFECTS OF LOCAL PURSLANE (Portulaca oleracea) LEAF CRUDE EXTRACTS ON NORMAL AND CANCER CELL LINES in Vitro

AS¹,

JH²

2013

Int J of Drug Disc

View full text Add to dashboard Cite

include cancers, and other health complains like respiratory, ophthalmological, dermatological, reproductive, and immunological complains [4]. It is widely held that (80-90%), of human cancers may be attributable to environmental, lifestyle factors such as tobacco, alcohol, and dietary habits [9,12]. Portulaca oleracea L. (Purslane) has been used as a kind of food and medicinal plant for thousands of years in China. P. oleracea is very important because of its special medical function and all its therapeutic values are attributed to the presence of many biologically active compounds which include; flavonoids (Apigenin, kaempferol, quercetin, luteolin, myricetin, genistein, and genistin), Alkaloids, Coumarins, anthraquinone glycoside, cardiac glycoside, and high content of ω-3 fatty acids [13].

show abstract

In vitrocytotoxic activity ofBaccharis dracunculifoliaand propolis against HEp-2 cells

Búfalo

Candeias

Sousa

et al. 2010

Natural Product Research

View full text Add to dashboard Cite

Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures

Abstract: ABSTRACT

Cited by 3 publications

References 8 publications

In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp‐2) Cells

In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp‐2) Cells

CYTOGENETIC TOXICITY EFFECTS OF LOCAL PURSLANE (Portulaca oleracea) LEAF CRUDE EXTRACTS ON NORMAL AND CANCER CELL LINES in Vitro

In vitrocytotoxic activity ofBaccharis dracunculifoliaand propolis against HEp-2 cells

Contact Info

Product

Resources

About