Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions

Melzak, Kathryn A.; Sherwood, Christopher; Turner, Robin F. B.; Haynes, Charles A.

doi:10.1006/jcis.1996.0421

Cited by 340 publications

(363 citation statements)

References 13 publications

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“…The good correspondence between the measured adsorption isotherm for NA in 3M NaClO 4 and the measurements of ref [13] for different silica properties supports our earlier discussion that adsorption isotherm data can be applied to sources of nonporous silica having different mean particle size.…”

Section: Na Adsorption Isothermssupporting

confidence: 64%

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Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit

López

Fuller

et al. 2008

Analytical Biochemistry

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We report nucleic acid (NA) adsorption isotherms and elution profiles for silica surfaces and use these to design a miniaturized NA purification unit based on solid-phase extraction with silica beads. The procedure is based on a pressure drop equation for flow through a packed bed and allows estimation of key design parameters such as channel dimensions, liquid flow rates, sample volume and amount of silica needed. The usefulness of this design procedure is shown by applying it to a column-based NA purification device for influenza detection for a case study of Madin-Darby canine kidney (MDCK) cells infected with influenza A virus.

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Section: Na Adsorption Isothermssupporting

confidence: 64%

“…Adsorption measurements were obtained with the procedure outlined by Melzak et al [13]. For samples containing chaotropic salts with high absorbances at a wavelength of 260 nm, the aliquot was processed with the QIAamp kit prior to UV measurement.…”

Section: Nucleic Acid Adsorption Measurementsmentioning

confidence: 99%

Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit

López

Fuller

et al. 2008

Analytical Biochemistry

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“…The exact mechanism of DNA and SPS adsorption to silica under conditions of high ionic strength is not clear. One report indicates that DNA adsorption is due to weakened intermolecular electrostatic repulsion forces, dehydration of the DNA and silica surfaces, and intermolecular hydrogen bond formation in the DNA-silica contact layer, 37 whereas other reports claim that cations such as sodium or guanidinium disrupt the hydration sphere around DNA and that these positively charged ions form a salt bridge between the negatively charged silica and the DNA backbone. 38 In contrast to the silica fibers of the spin columns, the surface of the magnetic particles contained within the cartridges of the PrepFiler Express forensic DNA extraction kit were embedded with a hydrophilic polymer.…”

Section: Discussionmentioning

confidence: 99%

A Sample Extraction Method for Faster, More Sensitive PCR-Based Detection of Pathogens in Blood Culture

Regan¹,

Furtado²,

Brevnov³

et al. 2012

The Journal of Molecular Diagnostics

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Three mechanistically different sample extraction methodologies, namely, silica spin columns, phenolchloroform, and an automated magnetic capture of polymer-complexed DNA (via an Automate Express instrument), were compared for their abilities to purify nucleic acids from blood culture fluids for use in TaqMan assays for detection of Staphylococcus aureus. The extracts from silica columns required 100-to 1000-fold dilutions to sufficiently reduce the powerful PCR inhibitory effects of the anticoagulant sodium polyanetholsulfonate, a common additive in blood culture media. In contrast, samples extracted by either phenol-chloroform or the Automate Express instrument required little or no dilution, respectively, allowing for an approximate 100-fold improvement in assay sensitivity. Analysis of 60 blood culture bottles indicated that these latter two methodologies could be used to detect lower numbers of pathogens and that a growing S. aureus culture could be detected 2 hours earlier than when using silica columns. Of the three tested methodologies, the Automate Express instrument had the shortest time to result, requiring only approximately 80 minutes to process 12 samples. These findings highlight the importance of considering the mechanism when selecting a DNA extraction methodology, given that certain PCR inhibitors act in a similar fashion to DNA in certain chemical environments, resulting in copurification, whereas other methodologies use different chemistries that have advantages during the DNA purification of certain types of samples. ( J Mol Diagn

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“…The same question was formulated by several authors. 690,[696][697][698][699] Summarizing the results of these different works, one may conclude that the energetic contributions to DNA interaction with a silica surface can be categorized into three parts: i) the electrostatic effect (repulsive); ii) the dehydration effect; iii) DNA/silica Hbonding. As regards point i), it was observed that an increase of the salt concentration (namely, the ionic strength) led to an increase of adsorption, which was more pronounced for divalent than for monovalent cations.…”

Section: Interaction With Nucleic Acid-related Biomoleculesmentioning

confidence: 99%

Silica Surface Features and Their Role in the Adsorption of Biomolecules: Computational Modeling and Experiments

et al. 2013

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Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions

Cited by 340 publications

References 13 publications

Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit

Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit

A Sample Extraction Method for Faster, More Sensitive PCR-Based Detection of Pathogens in Blood Culture

Silica Surface Features and Their Role in the Adsorption of Biomolecules: Computational Modeling and Experiments

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