DRL1, Encoding A NAC Transcription Factor, Is Involved in Leaf Senescence in Grapevine

Zhu, Ziguo; Li, Gui-Rong; Yan, Chaohui; Liu, Li; Zhang, Qingtian; Han, Zhen; Li, Bo

doi:10.3390/ijms20112678

Cited by 21 publications

(16 citation statements)

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“…Overexpression of DRL1 in tobacco has been shown to delay leaf senescence and decrease ABA levels. The expression of NtZEP1 and carotenoid cleavage genes is reduced in these transgenic plants (Zhu et al, 2019). However, the endogenous function of DRL1 in grapevine is yet to be reported.…”

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confidence: 99%

Transcriptional Regulation of Carotenoid Biosynthesis in Plants: So Many Regulators, So Little Consensus

2019

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In plants, the carotenoid biosynthesis pathway (CBP) is essential for the production of photosynthetic and protective pigments, plant hormones, and visual/olfactory attractants for animal pollinators and seed dispersers. The regulation of carotenoid biosynthesis at the transcriptional level is vitally important for all of these functions and has been the subject of intensive research. Many putative transcriptional regulators, both direct and indirect, have been identified through conventional mutant analysis, transcriptome profiling, yeast one-hybrid screening, and candidate gene approaches. Despite this progress, our understanding of the transcriptional regulation of carotenoid biosynthesis remains fragmented and incomplete. Frequently, a stimulus or regulator is known, but the mechanism by which it affects transcription has not been elucidated. In other cases, mechanisms have been proposed (such as direct binding of a CBP gene promoter by a transcription factor), but function was tested only in vitro or in heterologous systems, making it unclear whether these proteins actually play a role in carotenoid regulation in their endogenous environments. Even in cases where the mechanism is relatively well understood, regulators are often studied in isolation, either in a single plant species or outside the context of other known regulators. This presents a conundrum: why so many candidate regulators but so little consensus? Here we summarize current knowledge on transcriptional regulation of the CBP, lay out the challenges contributing to this conundrum, identify remaining knowledge gaps, and suggest future research directions to address these challenges and knowledge gaps.

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Section: Introductionmentioning

confidence: 99%

Transcriptional Regulation of Carotenoid Biosynthesis in Plants: So Many Regulators, So Little Consensus

2019

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“…NACs are a plant-specific transcription factor family whose name is derived from the first letters of the petunia (Petunia hybrida) NAM gene and the Arabidopsis thaliana ATAF1/2 and CUC2 genes (Riechmann et al, 2000). The NAC family is one of the largest gene families in plants (Guo et al, 2015;Hu et al, 2010;Olsen et al, 2005), and its members are involved in various stages of plant growth and development, including the formation of apical meristems (Souer et al, 1996), embryonic development (Kunieda et al, 2008), floral organ morphogenesis (Sablowski and Meyerowitz, 1998;Sanders et al, 1999), flowering time regulation (Guo et al, 2018), lateral root initiation (Sohrabi et al, 2017), and leaf senescence (Zhu et al, 2019).…”

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“…XP-002281816) was identified from V. vinifera 'Yatomi Rosa' and shown to be associated with dwarfing and rolled leaf phenotypes (Yan et al, 2016). DRL1 also appears to have a role in senescence, because its overexpression in tobacco plants significantly delayed leaf senescence (Zhu et al, 2019). Here, we expanded upon this preliminary work by studying the effects of DRL1 on tobacco flowering and fruiting.…”

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Effects of Grape NAC Transcription Factor DRL1 on Floral Organogenesis in Tobacco

Li¹,

Quan²,

Yan³

et al. 2021

J. Amer. Soc. Hort. Sci.

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Grape (Vitis vinifera) is among the world’s most important fruit crops and is a commonly used woody plant for genomics and post-genomics research. NAC transcription factors play central roles in plant growth and development, floral organ morphogenesis, and responses to biological stress. It is therefore important to identify key transcription factors from grape and clarify their mechanisms of action to generate genetic resources for grape molecular improvement. Our research group previously cloned a NAC transcription factor from V. vinifera ‘Yatomi Rosa’ [drought and leaf roll gene 1 (DRL1)] and demonstrated that it caused dwarfing of tobacco (Nicotiana benthamiana) plants when overexpressed. In the present work, we demonstrate that overexpression of DRL1 in transgenic tobacco delays flowering time and markedly reduces pollen viability. Furthermore, crosses between male DRL1 transgenic tobacco and female wild-type tobacco exhibit substantially lower fruit set, fruit and seed weights, fruit and seed shape indices, and seed germination rates than selfed wild-type plants or crosses with a transgenic female parent. DLR1 overexpression strongly influences flowering time and reproduction in transgenic tobacco, primarily through its effects on pollen development. These results provide a foundation for further functional characterization of DLR1 in grape.

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“…NAC proteins are activators and/or repressors of gene expression and modulate plant development, plant defense and stress tolerance processes (for review see Olsen et al 13 , Nakano et al 14 , Kim et al 15 , Ohbayashi and Sugiyama 16 , Mathew and Agarwal 17 ). NAC proteins have been documented to be involved in leaf, petal and fruit senescence in Arabidopsis thaliana 3,15,[18][19][20][21][22][23][24][25][26][27][28][29][30] , Solanum lycopersicum [31][32][33] , Oryza sativa [34][35][36][37][38] , Hordeum vulgare 39,40 , Glycine max 41 , Bambusa emeiensis 42 , Trifolium pratense 43 , Helianthus annuus 44 , Gossypium hirsutum [45][46][47] , Musa x paradisiaca 48,49 , Vitis vinifera 50 and Nicotiana tabacum 51 . Among those, several NAC genes are linked to a stay-green phenotype.…”

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HEBE, a novel positive regulator of senescence in Solanum lycopersicum

Forlani

Cozzi

Rosa

et al. 2020

Sci Rep

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varieties, transgenic or knockout lines able to maintain their green color longer than wild-type plants. In these plants, long-lasting leaf coloration is correlated to durable chlorophyll accumulation compared to wild-type plants or standard varieties, and it is often associated with delayed senescence 52. In Arabidopsis, senescence mechanisms induce the expression of ORESARA1 (ORE1) and ORE1 SISTER1 (ORS1) genes. ORE1 activates program cell death and ORS1 participates to salt-induced senescence; the corresponding knockout mutant plants display a stay-green phenotype and delayed senescence 19,20,22,26. Conversely, the disruption of VND-INTERACTING2 (VNI2) and JUNGBRUNNEN1 (JUB1)-which also encode for two NAC proteins-causes early senescence while their overexpression induces a stay-green phenotype 24,53. Recently, it was demonstrated that transgenic tomato lines, with reduced accumulation of SlNAP2 messenger (Solanum lycopersicum NAC-like, activated by Apetala3/ Pistillata), display a stay-green phenotype even upon ABA (abscisic acid) application 33. In this manuscript, we describe the role of Solyc12g036480, which encodes a NAC transcription factor able to modulate leaf senescence in tomato. We demonstrate that Solyc12g036480 downregulation, achieved via Virusinduced gene silencing (VIGS), confers longer life span and delayed overall senescence in tomato plants; for this reason we named this gene HḖBĒ (HEB) after the Greek youth goddess. Results and discussion HEB expression analyses. The tomato NAC TFs family counts 101 members and only few of them have been functionally characterized. As yet, tomato NAC proteins have been described as involved in defense responses, stomata opening and closure, drought tolerance, flower-boundary morphogenesis, leaf senescence and fruit ripening 33,54-57. Among these 101 NAC members, we have selected Solyc12g036480/HEB for a deeper characterization. According to the transcriptome data collection of the Tomato Genome Consortium, HEB is equally transcribed in leaves and roots, but from the experimental data of Huang and Schiefelbein, HEB messenger is not detected in roots 58,59. In order to define temporally and spatially HEB expression pattern, quantitative Real-Time PCRs (qRT-PCRs) were performed. Expression analyses were carried out using organs dissected by Micro-tom plants; UBIQUITIN 3 (UBI3) and ELONGATION FACTOR 1α (EF1α) were used as reference genes 60. HEB transcript was found in young and old leaves and in young floral buds, but its mRNA is barely detected in roots, stem, mature green and red ripe fruits [developmental stages as described in 61 (Fig. 1)].

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DRL1, Encoding A NAC Transcription Factor, Is Involved in Leaf Senescence in Grapevine

Cited by 21 publications

References 72 publications

Transcriptional Regulation of Carotenoid Biosynthesis in Plants: So Many Regulators, So Little Consensus

Transcriptional Regulation of Carotenoid Biosynthesis in Plants: So Many Regulators, So Little Consensus

Effects of Grape NAC Transcription Factor DRL1 on Floral Organogenesis in Tobacco

HEBE, a novel positive regulator of senescence in Solanum lycopersicum

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