DROOPY LEAF1 controls leaf architecture by orchestrating early brassinosteroid signaling

Zhao, Meicheng; Tang, Sha; Zhang, Haoshan; He, Mei; Liu, Jihong; Zhi, Hui; Sui, Yi; Liu, Xiaotong; Jia, Guanqing; Zhao, Zhiyuan; Yan, Jijun; Zhang, Baocai; Zhou, Yihua; Chu, Jinfang; Wang, Xingchun; Zhao, Bin; Tang, Wenqiang; Li, Jiayang; Wu, Chuanyin; Liu, Xigang; Diao, Xianmin

doi:10.1073/pnas.2002278117

Cited by 48 publications

(30 citation statements)

References 35 publications

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“…At the 4-leaf stage, SiBRI1-OX seedlings in two independent lines, OX23 and OX14 , had larger leaf angles and droopier leaves than Ci846 seedlings (Fig. 5 A); notably, large leaf angles and drooping leaves have been reported to be specific phenotypes controlled by BR in foxtail millet [ 25 ]. Both of these phenotypes were dependent on the protein expression level of SiBRI1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet

et al. 2021

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Brassinosteroids (BRs) play important roles in plant growth and development. Although BR receptors have been intensively studied in Arabidopsis, those in foxtail millet remain largely unknown. Here, we show that the BR signaling function of BRASSINOSTEROID INSENSITIVE 1 (BRI1) is conserved between Arabidopsis and foxtail millet, a new model species for C4 and Panicoideae grasses. We identified four putative BR receptor genes in the foxtail millet genome: SiBRI1, SiBRI1-LIKE RECEPTOR KINASE 1 (SiBRL1), SiBRL2 and SiBRL3. Phylogenetic analysis was used to classify the BR receptors in dicots and monocots into three branches. Analysis of their expression patterns by quantitative real-time PCR (qRT-PCR) showed that these receptors were ubiquitously expressed in leaves, stems, dark-grown seedlings, roots and non-flowering spikelets. GFP fusion experiments verified that SiBRI1 localized to the cell membrane. We also explored the SiBRI1 function in Arabidopsis through complementation experiments. Ectopic overexpression of SiBRI1 in an Arabidopsis BR receptor loss-of-function mutant, bri1-116, mostly reversed the developmental defects of the mutant. When SiBRI1 was overexpressed in foxtail millet, the plants showed a drooping leaf phenotype and root development inhibition, lateral root initiation inhibition, and the expression of BR synthesis genes was inhibited. We further identified BRI1-interacting proteins by immunoprecipitation (IP)-mass spectrometry (MS). Our results not only demonstrate that SiBRI1 plays a conserved role in BR signaling in foxtail millet but also provide insight into the molecular mechanism of SiBRI1.

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Section: Resultsmentioning

confidence: 99%

“…These results indicated that the functional conservation of BRI1 among different species. Previous work has demonstrated that DROOPY LEAF1 (DPY1) participates in BR signaling and inhibits the interaction between SiBRI1 and SiBAK1 [ 25 ], but SiBRI1 in foxtail millet has not yet been characterized.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet

et al. 2021

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“…Due to our continuing efforts, positional cloning in Setaria is now practicable using “SSR41” and “Yugu1” EMS mutants as plant materials. To date, map-based cloning has been used to characterize at least eight S. italica genes, including SiMADS34 ( Hussin et al, 2021 ), DPY1 ( Zhao et al, 2020 ), SiSTL1 ( Tang et al, 2019 ), SiSTL2 ( Zhang et al, 2018a ), SiYGL1 ( Li et al, 2016 ), SiYGL2 ( Zhang et al, 2018b ), Loose Panicle1 ( Xiang et al, 2017 ), and SiAGO1b ( Liu et al, 2016 ). These studies illustrate that “SSR41” and “Yugu1” are suitable germplasm resources for forward genetics and positional cloning research on Setaria .…”

Section: Discussionmentioning

confidence: 99%

“…We propose that “SSR41” is an ideal genotype to match to “Yugu1” mutants to construct mapping populations, because “SSR41” is morphologically similar to, but highly genetically divergent from, “Yugu1”. To data, several genes of S. italica have been cloned using “SSR41” and the corresponding “Yugu1” mutant, including DPY1 ( Zhao et al, 2020 ), SiAGL1b ( Liu et al, 2016 ), SiSTL1 ( Tang et al, 2019 ), SiSTL2 ( Zhang et al, 2018a ), SiYGL1 ( Li et al, 2016 ), and SiYGL2 ( Zhang et al, 2018b ). In addition, “SSR41” is an elite cultivar with high yields, good grain quality, resistance to diverse diseases, and wide local adaptability, so it will be useful for breeding and crop improvement.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA Polymorphism Analysis and Molecular Marker Development for the Setaria italica Variety “SSR41” and Positional Cloning of the Setaria White Leaf Sheath Gene SiWLS1

et al. 2021

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Genome-wide DNA polymorphism analysis and molecular marker development are important for forward genetics research and DNA marker-assisted breeding. As an ideal model system for Panicoideae grasses and an important minor crop in East Asia, foxtail millet (Setaria italica) has a high-quality reference genome as well as large mutant libraries based on the “Yugu1” variety. However, there is still a lack of genetic and mutation mapping tools available for forward genetics research on S. italica. Here, we screened another S. italica genotype, “SSR41”, which is morphologically similar to, and readily cross-pollinates with, “Yugu1”. High-throughput resequencing of “SSR41” identified 1,102,064 reliable single nucleotide polymorphisms (SNPs) and 196,782 insertions/deletions (InDels) between the two genotypes, indicating that these two genotypes have high genetic diversity. Of the 8,361 high-quality InDels longer than 20 bp that were developed as molecular markers, 180 were validated with 91.5% accuracy. We used “SSR41” and these developed molecular markers to map the white leaf sheath gene SiWLS1. Further analyses showed that SiWLS1 encodes a chloroplast-localized protein that is involved in the regulation of chloroplast development in bundle sheath cells in the leaf sheath in S. italica and is related to sensitivity to heavy metals. Our study provides the methodology and an important resource for forward genetics research on Setaria.

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“…We recently showed that a decellularized leaf scaffold can be loaded with polylactic-co-glycolic acid (PLGA)-based rapamycin nanoparticles, these nanoparticle-perfused leaves could inhibit venous neointimal hyperplasia in a rat inferior vena cava (IVC) venoplasty model at day 14; decellularized onion cellulose can also be coated with PLGA rapamycin nanoparticles and inhibit venous neointimal hyperplasia (Bai et al, 2021a). Plant leaf has a very specific architecture, including leaf stem, leaf midrib, vascular system, vein, and cuticle (Fan et al, 2019;Zhao et al, 2020). This system is important for water and nutrient transportation and storage.…”

Section: Introductionmentioning

confidence: 99%

A Novel Plant Leaf Patch Absorbed With IL-33 Antibody Decreases Venous Neointimal hyperplasia

Xie

Bai

Sun

et al. 2021

Front. Bioeng. Biotechnol.

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Introduction: We recently showed that a decellularized leaf scaffold can be loaded with polylactic-co-glycolic acid (PLGA)-based rapamycin nanoparticles, this leaf patch can then inhibit venous neointimal hyperplasia in a rat inferior vena cava (IVC) venoplasty model. IL-33 plays a role in the neointimal formation after vascular injury. We hypothesized that plant leaves can absorb therapeutic drug solution and can be used as a patch with drug delivery capability, and plant leaves absorbed with IL-33 antibody can decrease venous neointimal hyperplasia in the rat IVC venoplasty model.Method: A human spiral saphenous vein (SVG) graft implanted in the popliteal vein was harvested from a patient with trauma and analyzed by immunofluorescence. Male Sprague-Dawley rats (aged 6–8 weeks) were used to create the IVC patch venoplasty model. Plant leaves absorbed with rhodamine, distilled water (control), rapamycin, IL-33, and IL-33 antibody were cut into patches (3 × 1.5 mm2) and implanted into the rat IVC. Patches were explanted at day 14 for analysis.Result: At day 14, in the patch absorbed with rhodamine group, immunofluorescence showed rhodamine fluorescence in the neointima, inside the patch, and in the adventitia. There was a significantly thinner neointima in the plant patch absorbed with rapamycin (p = 0.0231) compared to the patch absorbed with distilled water. There was a significantly large number of IL-33 (p = 0.006) and IL-1β (p = 0.012) positive cells in the human SVG neointima compared to the human great saphenous vein. In rats, there was a significantly thinner neointima, a smaller number of IL-33 (p = 0.0006) and IL-1β (p = 0.0008) positive cells in the IL-33 antibody-absorbed patch group compared to the IL-33-absorbed patch group.Conclusion: We found that the natural absorption capability of plant leaves means they can absorb drug solution efficiently and can also be used as a novel drug delivery system and venous patch. IL-33 plays a role in venous neointimal hyperplasia both in humans and rats; neutralization of IL-33 by IL-33 antibody can be a therapeutic method to decrease venous neointimal hyperplasia.

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DROOPY LEAF1 controls leaf architecture by orchestrating early brassinosteroid signaling

Cited by 48 publications

References 35 publications

Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet

Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet

Genome-Wide DNA Polymorphism Analysis and Molecular Marker Development for the Setaria italica Variety “SSR41” and Positional Cloning of the Setaria White Leaf Sheath Gene SiWLS1

A Novel Plant Leaf Patch Absorbed With IL-33 Antibody Decreases Venous Neointimal hyperplasia

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