Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

Chang, Yaw-Jen; Yang, Hong-Wei; Yao, Len-Hao; Yang, Wen-Tung

doi:10.1177/2472630319879647

SLAS Technology

2020

DOI: 10.1177/2472630319879647

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Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

Yaw-Jen Chang

Hong-Wei Yang

Len-Hao Yao

et al.

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References 33 publications

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Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Huang

et al. 2023

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Histidine tag (His-tag) is a representative epitope tag, which has been widely used in the purification or immobilization of recombinant proteins. In this study, a novel probe CTPE-IDA-Ni 2 + based on intramolecular rigidification induced emission for fluorescence detecting His-tagged proteins is successfully developed and experimentally demonstrated. This detecting system is based on TPE derivative incorporated IDA-Ni 2 + moiety for real-time detection of His-tagged proteins. In the presence of His-tagged proteins, the IDA-Ni 2 + -His-tag complex was formed, which restricted the rotation of two phenyl rings linked with TPE and subsequently resulting in the fluorescence turn on. Results have shown that our probe could directly detect His-tagged proteins with good selectivity and fast response within 10 min. In addition, we have proved that this system could be used in fluorescent labeling of His-tagged protein instead of traditional Western blot assay with simple procedure, high quality and low background interference, as well as without aggregation-caused quenching (ACQ) problems like some organic dyes.

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Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Huang

et al. 2023

ChemistrySelect

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Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

Cited by 1 publication

References 33 publications

Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

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