Droplet encapsulation of electrokinetically-focused analytes without loss of resolution

Papadimitriou, Vasileios A.; Kruit, Stella-Maria Annette; Segerink, Loes; Eijkel, Jan C.T.

doi:10.1039/d0lc00191k

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…Microscale droplet generators have a wide range of applications in various industries including inkjet printers [1], microfluidic slug flow systems [2] and Lab-on-a-chip setups [3,4]. Microfluid droplet generators offer a much higher precision and repeatability compared to conventional microscale batch processes for the continuous production of highly monodispersed emulsions.…”

Section: Introductionmentioning

confidence: 99%

A New Micro Pump for Rapid and Accurate Microscale Droplet manipulation Using thermo-viscous Actuation

Mehrabi

Isfahani

2022

Scientia Iranica

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Current study proposes a novel mechanism for micro scale droplet generation. To this end, a new micro pump consisting of an expansion chamber with two nozzles at its left and right sides, is introduced which is capable of creating a unidirectional flow. In the current study, for the first time, thermo-Viscose expansion method is used to actuate the flow by assuming that the fluid is heated by using a laser beam source leading to expansion and movement of the fluid. Two-phase flow analysis using the VOF method showed that the proposed micro pump can consistently generate droplets with uniform and micro-scale dimensions. Keywords: Lab on a chip; Droplet; Thermo-Viscose expansion; Two-phase flow; Bio; Biofluidics 𝛼 𝑇 Volumetric thermal expansion coefficient th. Thermal 𝛼 Volume fraction function l Liquid 𝛿 𝑘𝑙 Kronecker delta g Gas 𝛿 𝑡ℎ.

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Section: Introductionmentioning

confidence: 99%

A New Micro Pump for Rapid and Accurate Microscale Droplet manipulation Using thermo-viscous Actuation

Mehrabi

Isfahani

2022

Scientia Iranica

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“…Encapsulation is a process with constantly growing number of applications in various fields of science and technology, including food industry (Madene et al 2006;Shahidi and Han, 1993), analytical chemistry (Papadimitriou et al, 2020), biotechnology (Betancor and Luckarift, 2008;Köster et al, 2008;Pierre, 2004;Yaakov et al, 2018) and biomedical engineering (Krishnan et al, 2014;. There are several methods of creating capsules -using chemical reactions and physical processes, some of them including the use of an electric field (Prüsse et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…On the one hand, this protects them from adverse environmental impact that causes their degradation (pH, temperature, oxidants) and simultaneously increases their bioavailability, allowing their direct delivery to the location of therapeutic action (e.g., via an injection directly into a cancerous tumor (Vakilinezhad et al, 2018)). A particularly advantageous method of preparing homogeneous emulsions is the microfluidic technique (in which the dispergation phase takes the form of droplets that are homogeneous in terms of size, with a diameter from several hundred nanometers to several hundred micrometers) (Eberhardt et al, 2019;Köster et al, 2008) used mainly to analyze samples on a micro scale via lab-on-achip type devices (Papadimitriou et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chemical and Process Engineering

2022

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The electrostatic impulse method is an established method for producing microbeads or capsules. Such particles have found application in biomedical engineering and biotechnology. The geometric properties of the droplets -constituting precursors of microbeads and capsules -can be precisely controlled by adjusting the geometry of the nozzle system, the physical properties and the flow rate of the fluids involved, as well as the parameters of the electrostatic impulse. In this work, a method of mathematical modeling of the droplet generation process using the electrostatic impulse method in a single nozzle system is presented. The developed mathematical model is an extension of the standard Volume of Fluid (VOF) model by addition of the effect of the electric field on the fluid flow. The model was implemented into the OpenFOAM toolkit for computational fluid dynamics (CFD). The performed CFD simulation results showed good agreement with experimental data. As a result, the influence of all process parameters on the droplet generation process was studied. The most significant change in droplet generation was caused by changing the electrostatic impulse strength. The presented modeling method can be used for optimization of process design and for studying the mechanisms of droplet generation. It can be extended to describe multi nozzle systems used for one-step microcapsule production.

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“…For example, droplet-based mass spectrometry of bioanalytes may require the removal of unwanted salts from droplets, while droplet-templated chemical or material synthesis may require the addition of reagents into droplets. The manipulation of droplets is broadly relevant to many applications, such as for the synthesis of nanoparticles − and Janus particles, , sample preparation, , biochemical assays, and sensing. , Several passive and active strategies or combinations thereof have been leveraged to manipulate droplet composition. Zaremba et al used programmable syringe pumps and passive elements such as metering and merging traps to generate droplets with fixed or periodically distributed concentrations .…”

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confidence: 99%

Electrokinetic Desalting and Salting of Water-in-Oil Droplets

Krishnamurthy,

Anand

2024

Anal. Chem.

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Droplet-based microfluidic platforms demand modifications to the droplet composition to facilitate reactions and analyses. However, limited techniques exist to modify the droplet contents post their generation. Here, ion transport across two ionexchange membranes possessing distinct selectivity is employed to introduce ions into (salt) or extract ions from (desalt) water-in-oil droplets. The ion concentration distribution and transport mechanisms are visualized using a precipitation reaction and a charged fluorescent tracer. Furthermore, current measurements reveal characteristic regimes in desalting and salting modes and demonstrate that the rates of ion transport linearly correlate with applied voltage and the ionic strength of the droplets. Importantly, up to 98% desalting efficiency is achieved. This technique advances droplet-based sample preparation through the straightforward manipulation of droplet contents.

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Droplet encapsulation of electrokinetically-focused analytes without loss of resolution

Abstract: Novel continuous or on-demand droplet generator for extraction of electrokinetically focused and separated analytes on chip. White scale bars – 100 μm.

Cited by 5 publications

References 42 publications

A New Micro Pump for Rapid and Accurate Microscale Droplet manipulation Using thermo-viscous Actuation

A New Micro Pump for Rapid and Accurate Microscale Droplet manipulation Using thermo-viscous Actuation

Chemical and Process Engineering

Electrokinetic Desalting and Salting of Water-in-Oil Droplets

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