Droplet evaporation-induced analyte concentration toward sensitive biosensing

Wang, Yulu; Liu, Fei; Yang, Yuemeng; Xu, Li-Ping

doi:10.1039/d1qm00500f

Cited by 38 publications

(31 citation statements)

References 131 publications

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“…noted the benefits of such a ring-like deposition; they indicated that the “coffee-ring effect” partially separates analytes of interest from interfering molecules in the sample droplet . The “coffee-ring” pattern is advantageous for analyte concentration toward sensitive biosensing . In the present investigation, analyte accumulation at the droplet periphery results in intense signals from this area, which is advantageous when simply comparing the signal intensities of substrates.…”

Section: Resultsmentioning

confidence: 62%

“…57 The "coffee-ring" pattern is advantageous for analyte concentration toward sensitive biosensing. 58 In the present investigation, analyte accumulation at the droplet periphery results in intense signals from this area, which is advantageous when simply comparing the signal intensities of substrates. Signal reproducibility and uniform analyte distribution are beyond the scope of this study.…”

Section: Resultsmentioning

confidence: 98%

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Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Yamada

Murase

Yatsugi

et al. 2021

ACS Appl. Nano Mater.

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The ability to sensitively detect trace quantities of substances is important in many practical applications. Herein, the use of TiO 2 nanopillar arrays, formed using oblique electron-beam deposition and thermal treatment at 800 °C, as substrates for laser desorption/ionization mass spectrometry (LDI-MS) is reported. The TiO 2 nanopillar arrays effectively assist ionization of various analytes, including small amino acids, a sugar, pesticides, peptides, and proteins with molecular weights of up to 24,000. The fabricated surface nanostructures were analyzed by measuring contact angles and applying the Cassie−Baxter and Wenzel wetting models. The TiO 2 nanopillar surface density was shown to significantly affect the thermal properties of the substrate and the wettability of analyte-containing droplets. A TiO 2 nanopillared substrate with a lower surface density exhibited more intense signals for the detection of small (∼1.2 kDa) analytes. The use of the benzyl pyridinium ion ([BP] + ), as a thermometer chemical, revealed that TiO 2 substrates with the lower pillar density exhibited effective heat confinement due to the pillar morphology, which plays a significant role in increasing the signal intensities of small molecules. The wetting behavior of the droplet on the substrate revealed that analyte enrichment, facilitated by surface hydrophobization, is another factor that contributes to enhancing small-molecule LDI performance. In contrast, the homogeneous adsorption of target molecules onto the nanopillared surface is likely to be a dominant factor for the detection of proteins. In this work, it is demonstrated that the appearance and disappearance of "coffee rings" during the evaporation of droplets on the surface are closely related to analyte distribution and, consequently, the intensities of the detected LDI-MS signals. The heat confinement effect of the TiO 2 nanopillars, along with suitable sample wettability, delivers significant LDI performance.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 98%

Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Yamada

Murase

Yatsugi

et al. 2021

ACS Appl. Nano Mater.

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“…This strategy has been applied for ultrasensitive detection of different cancer biomarkers, such as free prostate-specific antigen (PSA) ( Chen et al, 2018 ) and mRNA ( Hu et al, 2017 ). For a comprehensive understanding of the development and biosensing application of superwettable micropatterns, several high-quality reviews can be found in the literature ( Xu et al, 2019 ; Wang et al, 2021 ).…”

Section: Different Types Of Superwettable Biosensorsmentioning

confidence: 99%

Superwettable Biosensor for Disease Biomarker Detection

Yang

Gao²

2022

Front. Bioeng. Biotechnol.

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Bioinspired superwettable materials have aroused wide interests in recent years for their promising application fields from service life to industry. As one kind of emerging application, the superwettable surfaces used to fabricate biosensors for the detection of disease biomarkers, especially tumor biomarkers, have been extensively studied. In this mini review, we briefly summarized the sensing strategy for disease biomarker detection based on superwettable biosensors, including fluorescence, electrochemistry, surface-enhanced Raman scattering, and visual assays. Finally, the challenges and direction for future development of superwettable biosensors are also discussed.

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“…When a droplet is placed on a common (hydrophilic or hydrophobic) surface, it spreads rapidly and leaves coffee-ring stain after the droplet completely evaporated. 26 , 27 To eliminate this problem, superhydrophobic surface is used as a powerful substrate for biosensing. Water droplet on the superhydrophobic surface shows a high contact angle (CA) larger than 150° with small contact area between the droplet and surface.…”

Section: Superwettable Biosensorsmentioning

confidence: 99%

Bio-inspired Superwettable Surface for the Detection of Cancer Biomarker: A Mini Review

Yang

Gao

2022

Technol Cancer Res Treat

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Inspired by nature, superwettable material-based biosensors have aroused wide interests due to their potential in cancer biomarker detection. This mini review mainly summarized the superwettable materials as novel biosensing substrates for the development of evaporation-induced enrichment-based signal amplification and visual biosensing method. Biosensing applications based on the superhydrophobic surfaces, superwettable micropatterned surfaces, and slippery lubricant-infused porous surfaces for various cancer biomarker detections were described in detail. Finally, an insight of remaining challenges and perspectives of superwettable biosensor is proposed.

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Droplet evaporation-induced analyte concentration toward sensitive biosensing

Abstract: Droplet evaporation is a natural phenomenon and a widely concerned topic in various research fields. Droplet-based biosensors have attracted much attention due to their promising application in clinical detection and...

Cited by 38 publications

References 131 publications

Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Superwettable Biosensor for Disease Biomarker Detection

Bio-inspired Superwettable Surface for the Detection of Cancer Biomarker: A Mini Review

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Droplet evaporation-induced analyte concentration toward sensitive biosensing

Abstract: Droplet evaporation is a natural phenomenon and a widely concerned topic in various research fields. Droplet-based biosensors have attracted much attention due to their promising application in clinical detection and...

Cited by 38 publications

References 131 publications

Laser Desorption/Ionization Mass Spectrometry Using TiO2 Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Laser Desorption/Ionization Mass Spectrometry Using TiO2 Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Superwettable Biosensor for Disease Biomarker Detection

Bio-inspired Superwettable Surface for the Detection of Cancer Biomarker: A Mini Review

Contact Info

Product

Resources

About

Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability

Laser Desorption/Ionization Mass Spectrometry Using TiO₂ Nanopillar Array Substrates with Tunable Surface Roughness and Wettability