Droplet microarray platforms for high-throughput drug screening

Shi, Lei; Liu, Sutong; Li, Xue; Huang, Xiwei; Luo, Hongyu; Bai, Qianwen; Zhu, Li; Wang, Lijun; Du, Xiaoxin; Jiang, Cheng; Liu, Shan; Li, Chen-Zhong

doi:10.1007/s00604-023-05833-9

Cited by 9 publications

(5 citation statements)

References 78 publications

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“…Regardless of the mutations harbored by the cells, the drug responses of primary tumor cells always reflect the direct responses of the tumor itself. However, traditional drug screening based on cell culture requires millions of cells, this being a bottleneck for applying it on primary tumor cells [49][50][51] .…”

Section: Discussionmentioning

confidence: 99%

Drug screening on digital microfluidics for cancer precision medicine

Zhai,

Liu,

et al. 2024

Nat Commun

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Drug screening based on in-vitro primary tumor cell culture has demonstrated potential in personalized cancer diagnosis. However, the limited number of tumor cells, especially from patients with early stage cancer, has hindered the widespread application of this technique. Hence, we developed a digital microfluidic system for drug screening using primary tumor cells and established a working protocol for precision medicine. Smart control logic was developed to increase the throughput of the system and decrease its footprint to parallelly screen three drugs on a 4 × 4 cm2 chip in a device measuring 23 × 16 × 3.5 cm3. We validated this method in an MDA-MB-231 breast cancer xenograft mouse model and liver cancer specimens from patients, demonstrating tumor suppression in mice/patients treated with drugs that were screened to be effective on individual primary tumor cells. Mice treated with drugs screened on-chip as ineffective exhibited similar results to those in the control groups. The effective drug identified through on-chip screening demonstrated consistency with the absence of mutations in their related genes determined via exome sequencing of individual tumors, further validating this protocol. Therefore, this technique and system may promote advances in precision medicine for cancer treatment and, eventually, for any disease.

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Section: Discussionmentioning

confidence: 99%

Drug screening on digital microfluidics for cancer precision medicine

Zhai,

Liu,

et al. 2024

Nat Commun

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“…• Suitable for a variety of assays, including genomic, transcriptomic, proteomic, and metabolic studies [ 125 ]…”

Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

“…Droplet‐based microfluidic technologies and their application to single‐cell workflows offer significant advantages such as high throughput, parallelization possibilities, controlled confinement of single cells, and significant reagent cost reduction. [ 125–127 ] Furthermore, droplets provide an ideal microenvironment for conducting a variety of biological assays, offering precise spatial and temporal resolution. For example, multiple recent publications have showcased the substantial analytical potential of DMF for single‐cell genome and transcriptome analyses.…”

Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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From deciphering infection and disease mechanisms to identifying novel biomarkers and personalizing treatments, the characteristics of individual cells can provide significant insights into a variety of biological processes and facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited in terms of cost, contamination risks, sample volumes, analysis times, throughput, sensitivity, and selectivity. Although microfluidic approaches have been suggested as a low‐cost, information‐rich, and high‐throughput alternative to conventional single‐cell isolation and analysis methods, limitations such as necessary off‐chip sample pre‐ and post‐processing as well as systems designed for individual workflows have restricted their applications. In this review, a comprehensive overview of recent advances in integrated microfluidics for single‐cell isolation and on‐chip analysis in three prominent application domains are provided: investigation of somatic cells (particularly cancer and immune cells), stem cells, and microorganisms. Also, the use of conventional cell separation methods (e.g., dielectrophoresis) in unconventional or novel ways, which can advance the integration of multiple workflows in microfluidic systems, is discussed. Finally, a critical discussion related to current limitations of integrated microfluidic single‐cell workflows and how they could be overcome is provided.

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“…One or more high-yielding strains can be screened from a library of mutant strains ( Hertzberg and Pope, 2000 ). Although the screening flux is indeed greatly improved compared to manual screening of strains, there are also major constraints, such as expensive instruments, inability to avoid cross-contamination, and a large difference in the efficiency and cost of data collection ( Shi et al, 2023 ). To address the above problems, it is revolutionary to use droplet microfluidics instead of well plates to realize high-throughput screening.…”

Section: A New Technology To Assist Sa Biosynthesis—droplet Microflui...mentioning

confidence: 99%

Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits

Lin,

Li,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

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Succinic acid (SA), one of the 12 top platform chemicals produced from biomass, is a precursor of various high value-added derivatives. Specially, 1 mol CO2 is assimilated in 1 mol SA biosynthetic route under anaerobic conditions, which helps to achieve carbon reduction goals. In this review, methods for enhanced CO2 fixation in SA production and utilization of waste biomass for SA production are reviewed. Bioelectrochemical and bioreactor coupling systems constructed with off-gas reutilization to capture CO2 more efficiently were highlighted. In addition, the techno-economic analysis and carbon sequestration benefits for the synthesis of bio-based SA from CO2 and waste biomass are analyzed. Finally, a droplet microfluidics-based high-throughput screening technique applied to the future bioproduction of SA is proposed as a promising approach.

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Droplet microarray platforms for high-throughput drug screening

Cited by 9 publications

References 78 publications

Drug screening on digital microfluidics for cancer precision medicine

Drug screening on digital microfluidics for cancer precision medicine

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits

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