Droplet microfluidic-based loop-mediated isothermal amplification (dLAMP) for simultaneous quantification of multiple targets

Tan, Ya-Ling; Wang, Tao; He, Jianjun; Jiang, Jian‐Hui

doi:10.1016/j.xpro.2022.101335

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Most classic techniques for nucleic acid detection, such as polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP), have been updated by the implementation of droplet-based technologies, enabling improvements in precision and throughput. [11][12][13] In addition to the most well-established and classic techniques, new methods such as clustered regularly interspaced short palindromic repeat (CRISPR)-based analysis have used droplet microfluidics to enhance their capabilities.…”

Section: Nucleic Acid Analysismentioning

confidence: 99%

Droplet microfluidics for single‐molecule and single‐cell analysis in research, diagnosis, and therapy

Sanchez Barea,

Kang

2024

Bulletin Korean Chem Soc

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During the last decade, molecular diagnostics has become increasingly important, especially during the SARS‐CoV‐2 pandemic. Advances in droplet‐based microfluidics, which divides samples into thousands of microdroplets, are responsible for high‐throughput data provision, increased analysis sensitivity, and improved performance to overcome challenges in controlling diseases and early diagnosis. Different targets, such as nucleic acids, proteins, or single cells, can be detected and analyzed in terms of their characteristics in a much more extensive and precise manner with the integration of droplet microfluidics into various molecular diagnostic techniques. In this review, we explore recent advances in the development of droplet microfluidics‐based molecular diagnostic techniques and future perspectives in the field.

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Section: Nucleic Acid Analysismentioning

confidence: 99%

Droplet microfluidics for single‐molecule and single‐cell analysis in research, diagnosis, and therapy

Sanchez Barea,

Kang

2024

Bulletin Korean Chem Soc

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“…While there have been recent efforts to improve the capabilities of existing ddLAMP systems [28][29][30][31], there is still scope for improvement. First, traditional chamber or flowbased ddLAMP systems involve complex fabrication processes such as multilayer soft lithography and chemical etching which are time and labor-intensive.…”

Section: Introductionmentioning

confidence: 99%

A Smartphone-Enabled Continuous Flow Digital Droplet LAMP Platform for High Throughput and Inexpensive Quantitative Detection of Nucleic Acid Targets

Ditchendorf,

Ahmed,

Sepate

et al. 2023

Sensors

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Molecular tests for infectious diseases and genetic anomalies, which account for significant global morbidity and mortality, are central to nucleic acid analysis. In this study, we present a digital droplet LAMP (ddLAMP) platform that offers a cost-effective and portable solution for such assays. Our approach integrates disposable 3D-printed droplet generator chips with a consumer smartphone equipped with a custom image analysis application for conducting ddLAMP assays, thereby eliminating the necessity for expensive and complicated photolithographic techniques, optical microscopes, or flow cytometers. Our 3D printing technique for microfluidic chips facilitates rapid chip fabrication in under 2 h, without the complications of photolithography or chip bonding. The platform’s heating mechanism incorporates low-powered miniature heating blocks with dual resistive cartridges, ensuring rapid and accurate temperature modulation in a compact form. Instrumentation is further simplified by integrating miniaturized magnification and fluorescence optics with a smartphone camera. The fluorescence quantification benefits from our previously established RGB to CIE-xyY transformation, enhancing signal dynamic range. Performance assessment of our ddLAMP system revealed a limit of detection at 10 copies/μL, spanning a dynamic range up to 104 copies/μL. Notably, experimentally determined values of the fraction of positive droplets for varying DNA concentrations aligned with the anticipated exponential trend per Poisson statistics. Our holistic ddLAMP platform, inclusive of chip production, heating, and smartphone-based droplet evaluation, provides a refined method compatible with standard laboratory environments, alleviating the challenges of traditional photolithographic methods and intricate droplet microfluidics expertise.

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Detection and Analysis of Droplets in Microfluidic Devices: A Review

Yan,

Cheng,

Zou

et al. 2024

IEEE Sensors J.

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Droplet microfluidic-based loop-mediated isothermal amplification (dLAMP) for simultaneous quantification of multiple targets

Cited by 4 publications

References 23 publications

Droplet microfluidics for single‐molecule and single‐cell analysis in research, diagnosis, and therapy

Droplet microfluidics for single‐molecule and single‐cell analysis in research, diagnosis, and therapy

A Smartphone-Enabled Continuous Flow Digital Droplet LAMP Platform for High Throughput and Inexpensive Quantitative Detection of Nucleic Acid Targets

Detection and Analysis of Droplets in Microfluidic Devices: A Review

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