Droplet-vitrification for shoot tip cryopreservation of shallot (Allium cepa var. aggregatum): effects of PVS3 and PVS2 on shoot regrowth

Wang, Qiao‐Chun; Zhang, Zhibo; Zámečnı́k, Jiřı́; Bilavčík, Alois; Blystad, Dag‐Ragnar; Haugslien, Sissel

doi:10.1007/s11240-019-01721-4

Cited by 26 publications

(26 citation statements)

References 35 publications

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“…The present study was, therefore, to further assess rooting, vegetative growth, bulb production, genetic stability and contents of soluble sugars and flavonols in cryo-derived plants. Results reported here support use of the droplet-vitrification method described by Wang et al (2019Wang et al ( , 2020 for establishment of cryo-banks of shallot germplasm.…”

supporting

confidence: 77%

“…Subculturing was conducted once every 4 weeks. Shoot tips (2-3 mm in length) with 4-5 leaf primordia (LPs) were taken from 4-week old in vitro stock cultures and used for droplet-vitrification, as described by Wang et al (2020). Briefly, shoot tips were precultured, loaded and exposed to PVS3 (Nishizawa et al, 1993) at 24 o C for 3 h. At the end of exposure to PVS3, each of shoot tips was moved into 5 µl PVS3 droplets made on aluminum foils (2 x 0.8 cm), followed by direct immersion in LN for storage for 1 h. provided by cool-white fluorescent tubes.…”

Section: Preparation Of Cryo-derived Regenerantsmentioning

confidence: 99%

“…In the genus Allium, cryopreservation has been well-established for garlic (Keller 2002(Keller , 2005Keller et al 2011;Ellis et al 2006;Kim et al 2006Kim et al , 2007Liu et al 2017), while quite a few addressed shallot (Kim et al 2007). We previously reported a droplet-vitrification for efficient cryopreservation of shallot shoot tips (Wang et al 2019(Wang et al , 2020. The present study was, therefore, to further assess rooting, vegetative growth, bulb production, genetic stability and contents of soluble sugars and flavonols in cryo-derived plants.…”

mentioning

confidence: 99%

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Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Wang

Hamborg

Elameen

et al. 2020

Plant Cell Tiss Organ Cult

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supporting

confidence: 77%

Section: Preparation Of Cryo-derived Regenerantsmentioning

confidence: 99%

mentioning

confidence: 99%

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Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Wang

Hamborg

Elameen

et al. 2020

Plant Cell Tiss Organ Cult

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“…Occasionally, other explant types are also used for LN storage. Wang et al [ 47 ] reported 56%, 72%, and 32% shoot regrowth in cryopreserved shoot tips taken from in vitro shoots, adventitious buds regenerated from stem discs, and field-grown bulbs in onion ( Allium cepa L.), respectively. Chen and Wang [ 48 ] developed a cryopreservation protocol for cell suspensions and protoplasts of carrot ( Daucus carota L.), while Fábián et al [ 49 ] used wheat ( Triticum aestivum L.) egg cells excised from the ovaries.…”

Section: Plant Materials Used For Cryostoragementioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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“…Reed 2014, Mathew et al 2019. To use PVS3 instead of PVS2 (Wang et al 2019) or to alternation of recovery medium (Chang and Reed 1999) may help to improve the cryoprotocol.…”

Section: First Recovery Mediummentioning

confidence: 99%

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

Rantala

Kaseva

Nukari

et al. 2020

Plant Cell Tiss Organ Cult

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The aim of plant gene banks is to preserve genetic resources selected based on their phenotypic, agronomic, historical or other cultural values for future utilization. In the present study the modified PVS2 droplet vitrification technique was tested and optimized for cryopreservation of a large diversity of blackcurrant (R. nigrum L.) accessions propagated in vitro and selected into a national gene bank core collection. Out of four accessions tested to optimize the method, three recovered and regenerated by 89–97% on average, but one recalcitrant in vitro line only by 25%. The tested post-cryopreservation recovery media with different macronutrient and growth regulator levels showed no generalized effect on regenerated shoots, but the effect of recovery media was different between cultivars. When the whole regeneration chain from cryopreservation via micropropagation to greenhouse conditions was tested, shoots at least 1 cm in length were found necessary for successful transfer ex vitro. The long-term cryopreservation of 22 blackcurrant accessions was finally conducted, with practices slightly modified from the tested protocol. The estimated recovery of shoot tips after 9 weeks in vitro was 17–94% with at least 75% recovery in seven accessions and at least 40% recovery in 19 out of 22 accessions. Only one accession had no cryopreservation success. The results demonstrated that the modified droplet vitrification technique is appropriate for a large diversity of blackcurrant accessions. However, cultivar-related differences and recovery procedures are to be considered for success in regeneration and ex vitro adaptation.

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Droplet-vitrification for shoot tip cryopreservation of shallot (Allium cepa var. aggregatum): effects of PVS3 and PVS2 on shoot regrowth

Cited by 26 publications

References 35 publications

Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

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