Drosophila CLASP regulates microtubule orientation and dendrite pruning by suppressing Par-1 kinase

Bu, Shufeng; Tang, Quan; Wang, Yan; Lau, Samuel Song Yuan; Yong, Wei Lin; Yu, Fengwei

doi:10.1016/j.celrep.2022.110887

Cited by 4 publications

(4 citation statements)

References 70 publications

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“…However, in axons, MTs are arranged with nearly uniform plus-end-out orientation [33,34]. Interfering with the orientation of MTs in dendrites leads to defective dendrite pruning [35][36][37][38][39] (figure 1). More MT stabilizing and MT polarity-regulating proteins involved in dendritic pruning need to be discovered in the future.…”

Section: Jnk Pathway Microtubule Stabilization and Microtubule Orient...mentioning

confidence: 99%

Recent progress in dendritic pruning of Drosophila C4da sensory neurons

Rui

2024

Open Biol.

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The brain can adapt to changes in the environment through alterations in the number and structure of synapses. During embryonic and early postnatal stages, the synapses in the brain undergo rapid expansion and interconnections to form circuits. However, many of these synaptic connections are redundant or incorrect. Neurite pruning is a conserved process that occurs during both vertebrate and invertebrate development. It requires precise spatiotemporal control of local degradation of cellular components, comprising cytoskeletons and membranes, refines neuronal circuits, and ensures the precise connectivity required for proper function. The Drosophila ’s class IV dendritic arborization (C4da) sensory neuron has a well-characterized architecture and undergoes dendrite-specific sculpting, making it a valuable model for unravelling the intricate regulatory mechanisms underlie dendritic pruning. In this review, I attempt to provide an overview of the present state of research on dendritic pruning in C4da sensory neurons, as well as potential functional mechanisms in neurodevelopmental disorders.

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Section: Jnk Pathway Microtubule Stabilization and Microtubule Orient...mentioning

confidence: 99%

Recent progress in dendritic pruning of Drosophila C4da sensory neurons

Rui

2024

Open Biol.

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“…The fly da sensory neuron has been used as an in vivo model to study molecular mechanisms of dendritic morphogenesis [41]. Several microtubulerelated proteins have been found to play important roles in regulating the morphology and functions of dendrites during development [23,31,[40][41][42][43]. We examined whether Atat-mediated tubulin acetylation regulates dendritic development.…”

Section: K40 α-Tubulin Acetylation Regulates the Morphology Of Class ...mentioning

confidence: 99%

“…Microtubules are essential structural components of dendrites, and various signaling pathways regulate dendritic development by affecting microtubule dynamics [26,27,41,43,[49][50][51]. Microtubule PTMs, including acetylation, tyrosination, phosphorylation, polyglutamylation, and polyglycylation, are thought to adjust the dynamics and stability of microtubules [52].…”

Section: Tubulin K40 Acetylation Tunes the Development And Function O...mentioning

confidence: 99%

α-Tubulin acetylation at lysine 40 regulates dendritic arborization and larval locomotion by promoting microtubule stability in Drosophila

Niu

Mao²,

Wang³

et al. 2023

PLoS ONE

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Posttranslational modification of tubulin increases the dynamic complexity and functional diversity of microtubules. Acetylation of α-tubulin at Lys-40 is a highly conserved posttranslational modification that has been shown to improve the flexibility and resilience of microtubules. Here we studied the in vivo functions of α-tubulin acetylation by knocking-out Atat, the Drosophila α-tubulin acetyltransferase, and by mutating Lys-40 to Arg in α1-tubulin. We found a reduction in the dendritic arborization of larval class I dendritic arborization (da) neurons in both mutants. The dendritic developmental defects in atat mutants could be reversed by enhancing the stability of microtubules either through knocking down the microtubule severing protein Katanin 60 or through overexpressing tubulin-specific chaperone E, suggesting that α-tubulin deacetylation impairsed dendritic morphology by decreasing the stability of microtubules. Using time-lapse recordings, we found that atat and α1-tubulinK40R mutations dramatically increased the number of dendritic protrusions that were likely to be immature dendritic precursors. Finally, we showed that both Atat and α-tubulin acetylation were required in class I da neurons to control larval locomotion. These findings add novel insight into the current knowledge of the role of α-tubulin acetylation in regulating neuronal development and functions.

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“…These pruning factors lead to disassembly of actin (Kirilly et al, 2009;Wolterhoff et al, 2020) and microtubules (Herzmann et al, 2017;Lee et al, 2009;Williams and Truman, 2005) as well as locally increased endocytosis (Kanamori et al, 2015) in proximal dendrites. Microtubule dynamics in dendrites are increased during the early pupal stage by fine-tuned activation of the kinase Par-1 (Bu et al, 2022;Herzmann et al, 2017). The "plus end-in" orientation of dendritic microtubules is a spatial cue that localizes these degenerative processes to proximal parts of the dendrites (Herzmann et al, 2018;Rumpf et al, 2019;Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Developmental pruning of sensory neurites by mechanical tearing in Drosophila

Krämer

Wolterhoff

Galic

et al. 2023

Journal of Cell Biology

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Mechanical forces actively shape cells during development, but little is known about their roles during neuronal morphogenesis. Developmental neurite pruning, a critical circuit specification mechanism, often involves neurite abscission at predetermined sites by unknown mechanisms. Pruning of Drosophila sensory neuron dendrites during metamorphosis is triggered by the hormone ecdysone, which induces local disassembly of the dendritic cytoskeleton. Subsequently, dendrites are severed at positions close to the soma by an unknown mechanism. We found that ecdysone signaling causes the dendrites to become mechanically fragile. Severing occurs during periods of increased pupal morphogenetic tissue movements, which exert mechanical forces on the destabilized dendrites. Tissue movements and dendrite severing peak during pupal ecdysis, a period of strong abdominal contractions, and abolishing ecdysis causes non-cell autonomous dendrite pruning defects. Thus, our data establish mechanical tearing as a novel mechanism during neurite pruning.

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Drosophila CLASP regulates microtubule orientation and dendrite pruning by suppressing Par-1 kinase

Cited by 4 publications

References 70 publications

Recent progress in dendritic pruning of Drosophila C4da sensory neurons

Recent progress in dendritic pruning of Drosophila C4da sensory neurons

α-Tubulin acetylation at lysine 40 regulates dendritic arborization and larval locomotion by promoting microtubule stability in Drosophila

Developmental pruning of sensory neurites by mechanical tearing in Drosophila

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