Drosophila Gut Immune Pathway Suppresses Host Development-Promoting Effects of Acetic Acid Bacteria

Lee, Jaegeun; Song, Xinge; Hyun, Bom; Jeon, Che Ok; Hyun, Seogang

doi:10.14348/molcells.2023.0141

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…A balanced compositional signature of gut microbiota is pivotal to the host health. Gut microbiota are emerging as key players in the regulation of critical metabolic pathways and maintenance of health [ 81 ], in humans, mice, and other hosts [ 82 , 83 , 84 ]. This crosstalk between gut microbiome and host relies mainly on the distinctive biologically active catabolites that are produced and act as metabolic modulators [ 85 ] and their alteration may play a role in the impairment of energy/metabolic homeostasis [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Dysbiosis Shaped by Cocoa Butter-Based Sucrose-Free HFD Leads to Steatohepatitis, and Insulin Resistance in Mice

Kochumon,

Malik,

Sindhu

et al. 2024

Nutrients

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Background: High-fat diets cause gut dysbiosis and promote triglyceride accumulation, obesity, gut permeability changes, inflammation, and insulin resistance. Both cocoa butter and fish oil are considered to be a part of healthy diets. However, their differential effects on gut microbiome perturbations in mice fed high concentrations of these fats, in the absence of sucrose, remains to be elucidated. The aim of the study was to test whether the sucrose-free cocoa butter-based high-fat diet (C-HFD) feeding in mice leads to gut dysbiosis that associates with a pathologic phenotype marked by hepatic steatosis, low-grade inflammation, perturbed glucose homeostasis, and insulin resistance, compared with control mice fed the fish oil based high-fat diet (F-HFD). Results: C57BL/6 mice (5–6 mice/group) were fed two types of high fat diets (C-HFD and F-HFD) for 24 weeks. No significant difference was found in the liver weight or total body weight between the two groups. The 16S rRNA sequencing of gut bacterial samples displayed gut dysbiosis in C-HFD group, with differentially-altered microbial diversity or relative abundances. Bacteroidetes, Firmicutes, and Proteobacteria were highly abundant in C-HFD group, while the Verrucomicrobia, Saccharibacteria (TM7), Actinobacteria, and Tenericutes were more abundant in F-HFD group. Other taxa in C-HFD group included the Bacteroides, Odoribacter, Sutterella, Firmicutes bacterium (AF12), Anaeroplasma, Roseburia, and Parabacteroides distasonis. An increased Firmicutes/Bacteroidetes (F/B) ratio in C-HFD group, compared with F-HFD group, indicated the gut dysbiosis. These gut bacterial changes in C-HFD group had predicted associations with fatty liver disease and with lipogenic, inflammatory, glucose metabolic, and insulin signaling pathways. Consistent with its microbiome shift, the C-HFD group showed hepatic inflammation and steatosis, high fasting blood glucose, insulin resistance, increased hepatic de novo lipogenesis (Acetyl CoA carboxylases 1 (Acaca), Fatty acid synthase (Fasn), Stearoyl-CoA desaturase-1 (Scd1), Elongation of long-chain fatty acids family member 6 (Elovl6), Peroxisome proliferator-activated receptor-gamma (Pparg) and cholesterol synthesis (β-(hydroxy β-methylglutaryl-CoA reductase (Hmgcr). Non-significant differences were observed regarding fatty acid uptake (Cluster of differentiation 36 (CD36), Fatty acid binding protein-1 (Fabp1) and efflux (ATP-binding cassette G1 (Abcg1), Microsomal TG transfer protein (Mttp) in C-HFD group, compared with F-HFD group. The C-HFD group also displayed increased gene expression of inflammatory markers including Tumor necrosis factor alpha (Tnfa), C-C motif chemokine ligand 2 (Ccl2), and Interleukin-12 (Il12), as well as a tendency for liver fibrosis. Conclusion: These findings suggest that the sucrose-free C-HFD feeding in mice induces gut dysbiosis which associates with liver inflammation, steatosis, glucose intolerance and insulin resistance.

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Section: Discussionmentioning

confidence: 99%

Gut Dysbiosis Shaped by Cocoa Butter-Based Sucrose-Free HFD Leads to Steatohepatitis, and Insulin Resistance in Mice

Kochumon,

Malik,

Sindhu

et al. 2024

Nutrients

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“…RNA extraction and complementary DNA (cDNA) synthesis of fly samples were performed as previously described ( Chung et al, 2017 , Lee et al, 2023 , Lee et al, 2022 ). Total RNAs were extracted from adult fly heads using Easy-Blue system (iNtRON Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

A comparison study of pathological features and drug efficacy between Drosophila models of C9orf72 ALS/FTD

Lee,

Jeong,

Kim

et al. 2024

Molecules and Cells

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“…PM 10, which is generally found in higher proportions than PM 2.5, primarily stems from forest fires, waste combustion, and industrial and agricultural waste (Tian et al 2021 ). While PM’s adverse effects on the respiratory (Téllez-Rojo et al 2000 ; Leikauf et al 2020 ; Misiukiewicz-Stepien and Paplinska-Goryca 2021 ; Albano et al 2022 ; Lee et al 2023 ) and cardiovascular systems (Hamanaka and Mutlu 2018 ; Tschofen et al 2019 ; Basith et al 2022 ; Ji 2022 ) have been extensively studied, recent evidence has revealed its detrimental impacts on the gastrointestinal (GI) tract (Vignal et al 2017 ; Pambianchi et al 2022 ), including the modulation of the gut microbiome. In addition to direct deposition in the respiratory system, PM can be indirectly deposited in the oropharynx through mucociliary clearance and via the swallowing of mucus and saliva (Mutlu et al 2018 ; Xie et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…The gut microbiota forms a complex ecosystem of microorganisms, and its stability and resilience are crucial ecological features for human health (Fassarella et al 2021 ). The gut ecosystem influences various essential physiological functions, including digestion, nutrient absorption, immune system development, and synthesis of certain vitamins and neurotransmitters, among other critical functions (Hillman et al 2017 ; Lee et al 2023 ; Yun and Hyun 2023 ). Nonetheless, the diversity and composition of the gut microbiome can be influenced by diet, genetics, age, and environmental exposures (Hasan and Yang 2019 ; Su and Liu 2021 ), which also includes PM exposure and its association with the GI diseases.…”

Section: Introductionmentioning

confidence: 99%

Effect of aerobic exercise and particulate matter exposure duration on the diversity of gut microbiota

Imdad,

Kim,

et al. 2024

Animal Cells and Systems

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Inhalation of ambient particulate matter (PM) can disrupt the gut microbiome, while exercise independently influences the gut microbiome by promoting beneficial bacteria. In this study, we analyzed changes in gut microbial diversity and composition in response to combined interventions of PM exposure and aerobic exercise, extending up to 12 weeks. This investigation was conducted using mice, categorized into five groups: control group (Con), exercise group (EXE), exercise group followed by 3-day exposure to PM (EXE + 3-day PM), particulate matter exposure (PM), and PM exposure with concurrent treadmill exercise (PME). Notably, the PM group exhibited markedly lower alpha diversity and richness compared to the Con group and our analysis of beta diversity revealed significant variations among the intervention groups. Members of the Lachnospiraceae family showed significant enhancement in the exercise intervention groups (EXE and PME) compared to the Con and PM groups. The biomarker Lactobacillus, Coriobacteraceae , and Anaerofustis were enriched in the EXE group, while Desulfovibrionaceae , Mucispirillum schaedleri , Lactococcus and Anaeroplasma were highly enriched in the PM group. Differential abundance analysis revealed that Paraprevotella , Bacteroides , and Blautia were less abundant in the 12-week PM exposure group than in the 3-day PM exposure group. Moreover, both the 3-day and 12-week PM exposure groups exhibited a reduced relative abundance of Bacteroides uniformis , SMB53 , and Staphylococcus compared to non-PM exposure groups. These findings will help delineate the possible roles and associations of altered microbiota resulting from the studied interventions, paving the way for future mechanistic research.

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Drosophila Gut Immune Pathway Suppresses Host Development-Promoting Effects of Acetic Acid Bacteria

Cited by 7 publications

References 39 publications

Gut Dysbiosis Shaped by Cocoa Butter-Based Sucrose-Free HFD Leads to Steatohepatitis, and Insulin Resistance in Mice

Gut Dysbiosis Shaped by Cocoa Butter-Based Sucrose-Free HFD Leads to Steatohepatitis, and Insulin Resistance in Mice

A comparison study of pathological features and drug efficacy between Drosophila models of C9orf72 ALS/FTD

Effect of aerobic exercise and particulate matter exposure duration on the diversity of gut microbiota

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