Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

Neal, Scott J.; Dolezal, Darin; Jusić, Nisveta; Pignoni, Francesca

doi:10.1186/s12861-019-0181-0

Cited by 8 publications

(10 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protein O -fucosyltransferase 1 (POFUT1) [ 7 , 10 ] catalyzes the O -fucosylation of properly folded EGFs containing the consensus sequence C 2 XXXX( S / T )C 3 , where C 2 and C 3 are the second and third conserved cysteines in the EGF [ 11 , 12 ]. O -fucosylation of NOTCH1 (N1) is essential for its trafficking and function [ 13 , 14 , 15 , 16 ]. In particular, the O -fucose residues on N1 EGF repeat numbers 8 and 12 (EGF8 and EGF12) are directly involved in the interaction of the receptor with its ligands [ 17 , 18 ], and loss of these residues strongly affects Notch signaling [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE

et al. 2021

View full text Add to dashboard Cite

Fringes are glycosyltransferases that transfer a GlcNAc to O-fucose residues on Epidermal Growth Factor-like (EGF) repeats. Three Fringes exist in mammals: LUNATIC FRINGE (LFNG), MANIC FRINGE (MFNG), and RADICAL FRINGE (RFNG). Fringe modification of O-fucose on EGF repeats in the NOTCH1 (N1) extracellular domain modulates the activation of N1 signaling. Not all O-fucose residues of N1 are modified by all Fringes; some are modified by one or two Fringes and others not modified at all. The distinct effects on N1 activity depend on which Fringe is expressed in a cell. However, little data is available on the effect that more than one Fringe has on the modification of O-fucose residues and the resulting downstream consequence on Notch activation. Using mass spectral glycoproteomic site mapping and cell-based N1 signaling assays, we compared the effect of co-expression of N1 with one or more Fringes on modification of O-fucose and activation of N1 in three cell lines. Individual expression of each Fringe with N1 in the three cell lines revealed differences in modulation of the Notch pathway dependent on the presence of endogenous Fringes. Despite these cell-based differences, co-expression of several Fringes with N1 demonstrated a dominant effect of LFNG over MFNG or RFNG. MFNG and RFNG appeared to be co-dominant but strongly dependent on the ligands used to activate N1 and on the endogenous expression of Fringes. These results show a hierarchy of Fringe activity and indicate that the effect of MFNG and/or RFNG could be small in the presence of LFNG.

show abstract

Section: Introductionmentioning

confidence: 99%

Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Brain lobes along with VNCs were immunostained as previously described [ 62 ] except that the fixation duration was increased to 35 mins. Immunocytochemistry for transfected S2 cells at log phase were performed as described previously [ 63 ]. Primary antibodies used are chicken anti-GFP (Catalog #GFP-1020, Aves Labs, Tigard, Oregon; 1:500–1000), rabbit anti-DesRed (Catalog #632392, Takara Bio USA, Inc., Mountain View, California; 1:250), rabbit anti-Dpn (1:500) (a gift from Dr. Y.N.…”

Section: Methodsmentioning

confidence: 99%

Homeodomain protein Six4 prevents the generation of supernumerary Drosophila type II neuroblasts and premature differentiation of intermediate neural progenitors

et al. 2021

View full text Add to dashboard Cite

In order to boost the number and diversity of neurons generated from neural stem cells, intermediate neural progenitors (INPs) need to maintain their homeostasis by avoiding both dedifferentiation and premature differentiation. Elucidating how INPs maintain homeostasis is critical for understanding the generation of brain complexity and various neurological diseases resulting from defects in INP development. Here we report that Six4 expressed in Drosophila type II neuroblast (NB) lineages prevents the generation of supernumerary type II NBs and premature differentiation of INPs. We show that loss of Six4 leads to supernumerary type II NBs likely due to dedifferentiation of immature INPs (imINPs). We provide data to further demonstrate that Six4 inhibits the expression and activity of PntP1 in imINPs in part by forming a trimeric complex with Earmuff and PntP1. Furthermore, knockdown of Six4 exacerbates the loss of INPs resulting from the loss of PntP1 by enhancing ectopic Prospero expression in imINPs, suggesting that Six4 is also required for preventing premature differentiation of INPs. Taken together, our work identified a novel transcription factor that likely plays important roles in maintaining INP homeostasis.

show abstract

“…The BMP/Dpp/TGFβ signal transduction pathway is important for the growth, proliferation, and differentiation of cells of imaginal discs (Peterson and O'Connor, 2014;Upadhyay et al, 2017). The Dad gene has two enhancers, the proximal Dad13 enhancer (the main enhancer) and the distal DadInt52 enhancer (shadow enhancer) (Weiss et al, 2010;Neal et al, 2019). Both enhancers are located in the introns of the gene (Figure 2A).…”

Section: Construction Of a Calibration Curve And Analysis Of The 3c L...mentioning

confidence: 99%

Application of the 3C Method to Study the Developmental Genes in Drosophila Larvae

Bylino

Ibragimov

Digilio³

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

A transition from one developmental stage to another is accompanied by activation of developmental programs and corresponding gene ensembles. Changes in the spatial conformation of the corresponding loci are associated with this activation and can be investigated with the help of the Chromosome Conformation Capture (3C) methodology. Application of 3C to specific developmental stages is a sophisticated task. Here, we describe the use of the 3C method to study the spatial organization of developmental loci in Drosophila larvae. We critically analyzed the existing protocols and offered our own solutions and the optimized protocol to overcome limitations. To demonstrate the efficiency of our procedure, we studied the spatial organization of the developmental locus Dad in 3rd instar Drosophila larvae. Differences in locus conformation were found between embryonic cells and living wild-type larvae. We also observed the establishment of novel regulatory interactions in the presence of an adjacent transgene upon activation of its expression in larvae. Our work fills the gap in the application of the 3C method to Drosophila larvae and provides a useful guide for establishing 3C on an animal model.

show abstract

Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

Cited by 8 publications

References 78 publications

Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE

Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE

Homeodomain protein Six4 prevents the generation of supernumerary Drosophila type II neuroblasts and premature differentiation of intermediate neural progenitors

Application of the 3C Method to Study the Developmental Genes in Drosophila Larvae

Contact Info

Product

Resources

About