Drosophila Models of Cardiac Aging and Disease

Sujkowski, Alyson; Wessells, Robert J.

doi:10.1007/978-3-319-18326-8_6

Cited by 3 publications

(6 citation statements)

References 87 publications

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“…Live videos tracking these movements with a timestamp can be used to quantify rate, rhythmicity, contractile volume change, contractile velocity, and relaxation velocity. Such methods have been used to study numerous genetic models of development and disease in the fly system ( 17 , 20 ). Trained flies typically show increased contractile volume change and contractile velocity compared with age-matched controls.…”

Section: Assays For Training Outcomes In Drosophilamentioning

confidence: 99%

“…Failure rate in response to pacing is a powerful measure of physiological aging because it increases steadily with age ( 20 ), is reduced by treatments that slow aging ( 1 , 5 , 6 ), and is a sensitive indicator of decline before any obvious increase in mortality. Exercise training slows the increase of failure rate with age in multiple genotypes ( 4 ) and can even rescue the failure rate of mutants with poor heart performance ( 6 ).…”

Section: Assays For Training Outcomes In Drosophilamentioning

confidence: 99%

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Using Drosophila to Understand Biochemical and Behavioral Responses to Exercise

Sujkowski

Wessells

2018

Exercise and Sport Sciences Reviews

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Section: Assays For Training Outcomes In Drosophilamentioning

confidence: 99%

Section: Assays For Training Outcomes In Drosophilamentioning

confidence: 99%

Using Drosophila to Understand Biochemical and Behavioral Responses to Exercise

Sujkowski

Wessells

2018

Exercise and Sport Sciences Reviews

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“…Exercise training is cardio-protective in wild-type males, as measured by response to external electrical pacing stress [31]. Both muscle-and heart-specific OAMB knockdown prevented cardioprotective benefits of exercise training, while RU-controls responded normally to exercise ( Fig 1E and 1K).…”

Section: Oamb Is Required In Muscle and Heart For Improved Climbing Smentioning

confidence: 92%

“…The percentage of fly hearts that responded to pacing with either fibrillation or arrest were recorded as "% failure". Pacing-induced failure rate is a marker for stress sensitivity and characteristically declines with age [31,58]. Endurance exercise reduces cardiac failure rate across ages in trained male Drosophila [7,11,31].…”

Section: Cardiac Pacingmentioning

confidence: 99%

Alpha- and beta-adrenergic octopamine receptors in muscle and heart are required for Drosophila exercise adaptations

et al. 2020

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Endurance exercise has broadly protective effects across organisms, increasing metabolic fitness and reducing incidence of several age-related diseases. Drosophila has emerged as a useful model for studying changes induced by chronic endurance exercise, as exercising flies experience improvements to various aspects of fitness at the cellular, organ and organismal level. The activity of octopaminergic neurons is sufficient to induce the conserved cellular and physiological changes seen following endurance training. All 4 octopamine receptors are required in at least one target tissue, but only one, Octβ1R, is required for all of them. Here, we perform tissue-and adult-specific knockdown of alpha-and beta-adrenergic octopamine receptors in several target tissues. We find that reduced expression of Octβ1R in adult muscles abolishes exercise-induced improvements in endurance, climbing speed, flight, cardiac performance and fat-body catabolism in male Drosophila. Importantly, Octβ1R and OAMB expression in the heart is also required cell-nonautonomously for adaptations in other tissues, such as skeletal muscles in legs and adult fat body. These findings indicate that activation of distinct octopamine receptors in skeletal and cardiac muscle are required for Drosophila exercise adaptations, and suggest that cell non-autonomous factors downstream of octopaminergic activation play a key role.

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“…While vertebrate models have correlated specific factors like Integrin to CHDs, cell culture and simpler genetic models are powerful tools to map the complex signaling networks surrounding these genes. In particular, here we highlight the fruit fly ( Drosophila melanogaster ), which has become a well-studied model for cardiovascular development and aging for experimental biologists (reviewed in [ 22 ]). Although far simpler anatomically, development of the Drosophila embryonic heart is reminiscent of early vertebrate heart morphogenesis [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Mapping Heart Development in Flies: Src42A Acts Non-Autonomously to Promote Heart Tube Formation in Drosophila

Vanderploeg

Jacobs

2017

Veterinary Sciences

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Congenital heart defects, clinically identified in both small and large animals, are multifactorial and complex. Although heritable factors are known to have a role in cardiovascular disease, the full genetic aetiology remains unclear. Model organism research has proven valuable in providing a deeper understanding of the essential factors in heart development. For example, mouse knock-out studies reveal a role for the Integrin adhesion receptor in cardiac tissue. Recent research in Drosophila melanogaster (the fruit fly), a powerful experimental model, has demonstrated that the link between the extracellular matrix and the cell, mediated by Integrins, is required for multiple aspects of cardiogenesis. Here we test the hypothesis that Integrins signal to the heart cells through Src42A kinase. Using the powerful genetics and cell biology analysis possible in Drosophila, we demonstrate that Src42A acts in early events of heart tube development. Careful examination of mutant heart tissue and genetic interaction data suggests that Src42A’s role is independent of Integrin and the Integrin-related Focal Adhesion Kinase. Rather, Src42A acts non-autonomously by promoting programmed cell death of the amnioserosa, a transient tissue that neighbors the developing heart.

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Drosophila Models of Cardiac Aging and Disease

Cited by 3 publications

References 87 publications

Using Drosophila to Understand Biochemical and Behavioral Responses to Exercise

Using Drosophila to Understand Biochemical and Behavioral Responses to Exercise

Alpha- and beta-adrenergic octopamine receptors in muscle and heart are required for Drosophila exercise adaptations

Mapping Heart Development in Flies: Src42A Acts Non-Autonomously to Promote Heart Tube Formation in Drosophila

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