Drought induces oxidative stress in pea plants

Morán, José F.; Becana, Manuel; Iturbe‐Ormaetxe, Iñaki; Frechilla, Silvia; Klucas, Robert V.; Tejo, Pedro María Aparicio

doi:10.1007/bf00197534

Cited by 535 publications

(348 citation statements)

References 38 publications

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“…Miyake and Asada (1996) reported that low levels of H 2 O 2 (2 μM) inactivate chlAPX within several seconds, when the level of ascorbic acid is too low for the APX catalytic cycle to operate. This phenomenon was previously reported in purslane (Yazici et al, 2007), jatropha (Silva et al, 2010), rice (Lee et al, 2001), pea (Moran et al, 1994), bean (Jebara et al, 2005), and the mangrove Bruguiera parviflora (Parida et al, 2004). CAT deactivation by salt stress may be due to the prevention of new enzyme synthesis (Feierabend et al, 1992) or CAT photo-inactivation (Polle, 1997).…”

Section: Discussionsupporting

confidence: 62%

Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress

Campos

Hsie

Granja

et al. 2012

Braz. J. Plant Physiol.

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Biodiesel is an alternative to petroleum diesel fuel. It is a renewable, biodegradable, and nontoxic biofuel. Interest in the production of biodiesel from Jatropha curcas L. seeds has increased in recent years, but the ability of J. curcas to grow in salt-prone areas, such as the Caatinga semiarid region, has received considerably meager attention. The aim of this study was to identify the main physiological processes that can elucidate the pattern of responses of J. curcas irrigated with saline water, which commonly occurs in the semiarid Caatinga region. This study measured the activity of the antioxidant enzymes involved in the scavenging of reactive oxygen species, which include catalase (CAT) and ascorbate peroxidase (APX), as well as malondialdehyde (MDA) levels. The levels of chlorophyll (Chl), carotenoids, amino acids, proline, and soluble proteins were also analyzed. The net carbon assimilation rate (P N ), stomata conductance (g s ), and transpiration rate (E) decreased with salt stress. The activities of CAT and APX were decreased, while H 2 O 2 and MDA levels as well as electrolyte leakage were significantly increased in salt-stressed plants compared to the untreated ones. These observations suggest that the ability of J. curcas plants resist to salt stress is associated with the activities of protective enzymes and their defensive functions. However, our results indicate that the reactive oxygen species scavenging system is not sufficient to protect J. curcas leaves against oxidative damage caused by salt stress, and, therefore, it cannot be treated as a salt tolerant plant species.

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Section: Discussionsupporting

confidence: 62%

Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress

Campos

Hsie

Granja

et al. 2012

Braz. J. Plant Physiol.

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“…In Glycyrrhiza uralensis, drought stress induced no change in MnSOD and FeSOD activities; however CuZnSOD showed most abundant activity (Pan et al 2006). Increase in the activity of CuZnSODs was also observed in rice and pea plants exposed to drought (Ke et al 2009;Moran et al 1994). Although MnSOD activity was not detected in above described species, transgenic plant species (alfalfa and rice) overexpressing MnSOD were reported as more drought tolerant compared to non-transgenic plants (McKersie et al 1996;Wang et al 2005).…”

Section: Discussionmentioning

confidence: 95%

Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

Ghorecha

Patel

Ingle

et al. 2013

Physiol Mol Biol Plants

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The current study analyses few important biochemical parameters and microRNA expression in two closely related species (wild but tolerant Ipomoea campanulata L. and cultivated but sensitive Jacquemontia pentantha Jacq.G.Don) exposed to water deficit conditions naturally occurring in the field. Under soil water deficit, both the species showed reduction in their leaf area and SLA as compared to well-watered condition. A greater decrease in chlorophyll was noticed in J. pentantha (~50 %) as compared to I. campanulata (20 %) under stress. By contrast, anthocyanin and MDA accumulation was greater in J. pentantha as compared to I. campanulata . Multiple isoforms of superoxide dismutases (SODs) with differing activities were observed under stress in these two plant species. CuZnSOD isoforms showed comparatively higher induction (~10-40 %) in I. campanulata than J. pentantha . MicroRNAs, miR398, miR319, miR395 miR172, and miR408 showed opposing expression under water deficit in these two plant species. Expression of miR156, miR168, miR171, miR172, miR393, miR319, miR396, miR397 and miR408 from either I. campanulata or J. pentantha or both demonstrated opposite pattern of expression to that of drought stressed Arabidopsis. The better tolerance of the wild species (I. campanulata) to water deficit could be attributed to lesser variations in chlorophyll and anthocyanin levels; and relatively higher levels of SODs than J. pentantha. miRNA expression was different in I. campanulata than J. pentantha.

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“…Previous agronomical, physiological, and biochemical studies strongly suggest that alfalfa is more drought tolerant than pea (Moran et al, 1994) and ureideproducing grain legumes (Sinclair and Serraj, 1995). Simplified representations of some metabolic routes examined in this work.…”

mentioning

confidence: 91%

“…B, Production of ROS and some major antioxidant systems in legume nodules. Adapted from Matamoros et al (2003) For example, photosynthesis was inhibited by 77% in pea leaves having a water potential (C w ) of 21.3 MPa (Moran et al, 1994), but only decreased by 28% in alfalfa leaves at a C w of 21.8 MPa (Rubio et al, 2002). This inhibition was accompanied by consistent decreases in antioxidant activities and soluble protein in pea leaves, but by either minor or no changes in alfalfa leaves.…”

mentioning

confidence: 99%

The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

et al. 2007

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Alfalfa (Medicago sativa) plants were exposed to drought to examine the involvement of carbon metabolism and oxidative stress in the decline of nitrogenase (N 2 ase) activity. Exposure of plants to a moderate drought (leaf water potential of 21.3 MPa) had no effect on sucrose (Suc) synthase (SS) activity, but caused inhibition of N 2 ase activity (243%), accumulation of succinate (136%) and Suc (158%), and up-regulation of genes encoding cytosolic CuZn-superoxide dismutase (SOD), plastid FeSOD, cytosolic glutathione reductase, and bacterial MnSOD and catalases B and C. Intensification of stress (22.1 MPa) decreased N 2 ase (282%) and SS (230%) activities and increased malate (140%), succinate (168%), and Suc (1435%). There was also up-regulation (mRNA) of cytosolic ascorbate peroxidase and down-regulation (mRNA) of SS, homoglutathione synthetase, and bacterial catalase A. Drought stress did not affect nifH mRNA level or leghemoglobin expression, but decreased MoFe-and Fe-proteins. Rewatering of plants led to a partial recovery of the activity (75%) and proteins (.64%) of N 2 ase, a complete recovery of Suc, and a decrease of malate (248%) relative to control. The increase in O 2 diffusion resistance, the decrease in N 2 ase-linked respiration and N 2 ase proteins, the accumulation of respiratory substrates and oxidized lipids and proteins, and the up-regulation of antioxidant genes reveal that bacteroids have their respiratory activity impaired and that oxidative stress occurs in nodules under drought conditions prior to any detectable effect on SS or leghemoglobin. We conclude that a limitation in metabolic capacity of bacteroids and oxidative damage of cellular components are contributing factors to the inhibition of N 2 ase activity in alfalfa nodules.

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Drought induces oxidative stress in pea plants

Cited by 535 publications

References 38 publications

Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress

Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress

Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

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