Drought resistance of tobacco overexpressing the AfNAC1 gene of Amorpha fruticosa Linn.

Abstract

Plants are often adversely affected by abiotic stresses such as drought, low temperature, and salinity during growth, and plant NAC-like transcription factors are involved in regulating growth and developmental processes in response to stresses such as drought and salinity. In this study, to investigate the function of AfNAC1, a co-expression network of AfNAC1 genes was constructed using gene expression data from the Chinese legume deciduous shrub, Amorpha fruticosa Linn. A 576 bp NAC transcription factor (AfNAC1 gene, MN180266) encoding 191 amino acids was isolated from Amorpha fruticosa seedlings by RT-PCR. qRT-PCR showed that the AfNAC1 gene was expressed in the roots, stems, leaves, and flowers of Amorpha fruticosa. However, drought stress significantly increased root expression, and the AfNAC1 protein was localized in the nucleus by green fluorescence detection. This study analyzed the drought resistance of overexpressing tobacco in depth. Under natural drought stress, the chlorophyll and antioxidant enzyme activities of overexpressing plants were significantly higher than those of wild-type (WT) plants, but the MDA content was lower than that of WT; after rehydration the Fv/Fm values of AfNAC1-overexpressing tobacco recovered faster than those of wild-type tobacco and rapidly reached the control levels; AfNAC1 may be involved in the regulation of the photosystem and indirectly in the regulation of the plant in response to drought stress.

First Report and Genetic Analysis of the Invasive Species A. fruticosa L. in Greece: A Combined Genetic and Regeneration Study

“…Results showed that all traits were influenced by accessions, a key that may also demonstrate genetic content that probably influences variation. Furthermore, studies have been conducted on the expression of genes associated with drought [25], phenolic constituents [26], proteomic analysis of symbiotic proteins [27], and cancer research [28,29]. The aforementioned research signifies from one point of view the significance of A. fruticosa, although we cannot eliminate the negative effects that it has on ecosystems due to its rapid expansion.…”

Transcriptome-wide identification of NAC (no apical meristem/Arabidopsis transcription activation factor/cup-shaped cotyledon) transcription factors potentially involved in salt stress response in garlic

“…Similar results were also observed for HuNAC20 and HuNAC25 , two novel NAC genes from pitaya ( Hu et al, 2022 ). AfNAC1 from Amorpha fruticosa may have a part in photosystem regulation and indirectly confer plant resistance to drought stress ( Li et al, 2022 ). By contrast, TaSNAC4-3D negatively regulated drought tolerance by bringing about increased oxidative damage and programmed cell death in wheat ( Ma et al, 2022 ).…”