Drought response transcriptomes are altered in poplar with reduced tonoplast sucrose transporter expression

Xue, Liang‐Jiao; Frost, Christopher J.; Tsai, Chung‐Jui

doi:10.1038/srep33655

Cited by 35 publications

(49 citation statements)

References 59 publications

(102 reference statements)

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“…In the passive symplasmic loader poplar, PtSUT4 is down-regulated (Fig. 4E), confirming its involvement in the drought response indicated by the study of knock-out plants (Frost et al, 2012;Xue et al, 2016). Also notable is the strong reduction in expression of Arabidopsis AtSUC1 ( Figure 4A), a type I SUT not directly involved in phloem loading, which was also down-regulated in response to drought stress.…”

Section: Sut Expression Responds To Abiotic Stresssupporting

confidence: 68%

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Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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Section: Sut Expression Responds To Abiotic Stresssupporting

confidence: 68%

“…In the responses to drought and salt stress, down-regulation of PtaSUT4 coincides with reduced Suc export from the leaf, indicated by the growth inhibition observed in these trees (Xue et al, 2016). PtaSUT4 is also down-regulated under elevated CO 2 even though export rates are higher.…”

Section: Poplar Sut4 Might Regulate Phloem Loadingmentioning

confidence: 94%

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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“…Consistent with the notion of functional versatility in Populus, PtaSUT4 expression has been found to respond differently to chronic and acute water stress (Frost et al 2012;Xue et al 50 2016; Pagliarani et al 2019). In addition, aquaporin expression differs between droughtstressed wild-type (WT) and SUT4-RNAi poplars, consistent with a link between sucrose 52 trafficking and changes in water flux (Xue et al 2016). Together, it appears that the physiological role of SUT4 includes export of sucrose to distant sinks for their nourishment, 54 modulation of hydraulic properties that affect transpiration, or a delicate balancing of both.…”

mentioning

confidence: 88%

“…Generation and characterization of the SUT4-RNAi lines in the hybrid poplar P. tremula × P. alba 78 INRA 717-1B4 background (717) were previously described (Payyavula et al 2011;Xue et al 2016). Single-node cuttings were grown in a glasshouse in 4 gallon tree pots (Hummert 80 International) containing commercial soil mixture (Fafard 3B) supplemented with Osmocote (15-9-12 NPK 4-month release) as previously described (Frost et al 2012;Xue et al 2016…”

Section: Plant Propagation and Growthmentioning

confidence: 99%

Defoliation-induced compensatory transpiration is compromised inSUT4-RNAiPopulus

Frost¹,

Tsai²

2020

Preprint

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Leaf sucrose contents are high in species of the genus Populus and other temperate tree taxa.Sucrose is subcellularly compartmentalized, but assumptions about the physiological relevance of such partitioning remain largely unexplored. In this study, the effects of partial defoliation treatments on water uptake, leaf gas exchange properties, non-structural carbohydrate abundance in source and sink organs, and growth were compared in poplars with normal or impaired intracellular sucrose trafficking. The tonoplast sucrose transporter PtaSUT4 is well expressed in leaves of P. tremula × P. alba (INRA 717-IB4), and its inhibition by RNAinterference (RNAi) is known to affect leaf sucrose abundance. After partial defoliation, maximum photosynthesis rates increased while intercellular CO 2 decreased with trajectories that were similar in wild type and SUT4-RNAi lines. Leaf transpiration increased more robustly in wild-type than RNAi plants, while leaf water content increased more in RNAi lines. Stomatal conductance did not differ between genotypes, nor did it increase with defoliation. Postdefoliation reductions in steady-state levels of sucrose, the major hexoses (glucose and fructose) and starch were similar in wild-type and SUT4-RNAi shoot sinks. Water uptake and stem growth after partial defoliation were not as well sustained in RNAi as in wild-type plants.The data suggest that vacuolar efflux of sucrose by PtaSUT4 more clearly facilitated adjustments in water uptake than sucrose export following leaf removal.

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“…In addition, the gravitropism experiment included a fully factorial sampling of wild-type (WT) plants, two ARBORKNOX2 mutants and a gibberellic acid (GA) hormone treatment. The second experiment sampled xylem and bark vascular tissues from upright-grown trees that were well watered, drought stressed or drought recovered plants (SRS616268-SRS616303; Xue et al, 2016). In addition, the drought experiment sampled both INRA 717-1B4 (WT) and a RNAi knockdown mutant of a sucrose transporter (SUT4; Potri.004G190400).…”

Section: Rna-seq Datasets and Processingmentioning

confidence: 99%

Identifying gene coexpression networks underlying the dynamic regulation of wood‐forming tissues in Populus under diverse environmental conditions

et al. 2017

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Trees modify wood formation through integration of environmental and developmental signals in complex but poorly defined transcriptional networks, allowing trees to produce woody tissues appropriate to diverse environmental conditions. In order to identify relationships among genes expressed during wood formation, we integrated data from new and publically available datasets in Populus. These datasets were generated from woody tissue and include transcriptome profiling, transcription factor binding, DNA accessibility and genome-wide association mapping experiments. Coexpression modules were calculated, each of which contains genes showing similar expression patterns across experimental conditions, genotypes and treatments. Conserved gene coexpression modules (four modules totaling 8398 genes) were identified that were highly preserved across diverse environmental conditions and genetic backgrounds. Functional annotations as well as correlations with specific experimental treatments associated individual conserved modules with distinct biological processes underlying wood formation, such as cell-wall biosynthesis, meristem development and epigenetic pathways. Module genes were also enriched for DNase I hypersensitivity footprints and binding from four transcription factors associated with wood formation. The conserved modules are excellent candidates for modeling core developmental pathways common to wood formation in diverse environments and genotypes, and serve as testbeds for hypothesis generation and testing for future studies.

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Drought response transcriptomes are altered in poplar with reduced tonoplast sucrose transporter expression

Cited by 35 publications

References 59 publications

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Defoliation-induced compensatory transpiration is compromised inSUT4-RNAiPopulus

Identifying gene coexpression networks underlying the dynamic regulation of wood‐forming tissues in Populus under diverse environmental conditions

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