Drought stress-induced oxidative stress and antioxidative responses in four wheat (<i>Triticum aestivum</i>L.) varieties

Chakraborty, Usha; Pradhan, Bhubaneswar

doi:10.1080/03650340.2010.533660

Cited by 49 publications

(25 citation statements)

References 39 publications

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“…Crushed samples were centrifuged at 2,415 g for 25 min. Whole experiment was done in minimal light conditions (Chakraborty and Pradhan, 2011). The reaction was completed as an intense yellow color started developing.…”

Section: Quantifi Cation Of H 2 Omentioning

confidence: 99%

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Sarkar¹,

Chakraborty²,

Chakraborty³

2016

JPSP

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The aim of this study is to understand the effect of elevated temperature on wheat seedlings of four cultivars, gayetri (GY), gandhari (GN), kedar (KD), PBW343 in terms of tolerance/susceptibility, along with antioxidative and biochemical responses. For this, seedlings of four cultivars were exposed to different temperatures ranging from 25°C to 40°C for 6 h. According to heat susceptibility index calculated, GN was found to be heat tolerant, PBW343 heat sensitive, and the other two, GY and KD moderately tolerant. Exposure to high temperature led to a gradual increase in membrane lipid peroxidation and hydrogen peroxide accumulation. H 2 O 2 and malonaldehyde accumulated to much higher amount in leaf tissues of PBW343 and GY than GN and KD. Among antioxidative enzymes, catalase, glutathione reductase, and ascorbate peroxidase activity initially increased at 35°C, followed by reduced activity under heat stress in all cultivars. However, peroxidase activity continued to increase under heat stress. Total chlorophyll and non-enzymatic antioxidant carotenoids, initially increased up to 30°C, following which there was a gradual decrease in these components. Compatible osmolytes -proline and total sugar level within cellular compartments were enhanced up to 35°C. Results suggested that wheat can endure heatinduced oxidative stress up to the certain period, manifested by elevation of non-enzymatic antioxidants and osmoprotectants, as well as upregulation of antioxidative enzymes.

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Section: Quantifi Cation Of H 2 Omentioning

confidence: 99%

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Sarkar¹,

Chakraborty²,

Chakraborty³

2016

JPSP

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“…The percentage inhibition of the absorbance of the solution of DPPH was determined by the formula as described by Chakraborty and Pradhan (2011). Total antioxidant activity was thus measured as free radical scavenging ability in terms of inhibition of absorbance by DPPH.…”

Section: Determination Of Lipid Peroxidationmentioning

confidence: 99%

“…All the extractions and estimations were done in minimal light conditions (Chakraborty and Pradhan, 2011).…”

Section: H 2 O 2 Quantification and Its In Situ Detectionmentioning

confidence: 99%

“…The levels of H 2 O 2 in the samples were determined by the use of extinction coefficient 0.28 µmol -1 cm -1 in the calculation. DAB staining technique described by Thordal-Christensen et al (1997) was used with some minor modifications (Chakraborty and Pradhan, 2011) to detect the in situ levels of H 2 O 2 in the leaf samples. Vacuum infiltration of the leaf discs (20 mm in diameter) was done with DAB (pH 3.8) at a rate of 1 mg/mL.…”

Section: H 2 O 2 Quantification and Its In Situ Detectionmentioning

confidence: 99%

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Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation

Chakraborty

Pradhan

2012

Braz. J. Plant Physiol.

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Five varieties of wheat (Triticum aestivum L.) -KW, UP 2752, PBW 343, SO and LV -were subjected to water stress and sampling was done on the 3 rd , 6 th and 9 th day of stress. RWC decline in KW, UP 2752 and PBW 343 (36.65, 42.34 and 40.75% respectively) was comparatively lesser than in LV and SO (52.93 and 52.67% respectively). In all varieties tested, three antioxidant enzymes (POX, APOX and GR) showed an initial increase. The activity of POX and GR increased with the increase in the duration of stress in KW, UP 2752 and PBW 343, while the activity of APOX declined. However, CAT and SOD showed an initial increase in these varieties, whereas it declined in SO and LV with increase in the period of stress. Accumulation of H 2 O 2 declined during prolonged water stress in KW, UP 2752 and PBW 343, while it increased in LV and SO. The accumulation of MDA content was three times higher in susceptible varieties than in tolerant varieties. The content of proline, phenol and ascorbate increased during water stress whereas the accumulation of carotenoid showed a significant decrease after showing an initial increase in the tested varieties. Higher values of total antioxidant and MSI were recorded in KW, UP 2752 and PBW 343 during stress while after 6 days MSI declined in LV and SO. During water stress there was a general decline in the total chlorophyll content. Analyzing the data, the present work suggested that out of the five varieties, KW, UP 2752 and PBW 343 showed more tolerance to water stress than SO and LV.Keywords: antioxidants, carotenoids, drought, lipid peroxidation.Abbreviations: APOX: ascorbate peroxidase (EC.1.11.1.11); CAT: catalase (EC.1.11.1.6); CMS: cell membrane stability; DAB: diaminobenzidine; EC: electrical conductivity; GR: glutathione reductase (EC 1.6.4.2); H 2 O 2 : hydrogen peroxide; KW: Kaweri; LV: local variety; MDA: malondialdehyde; MSI: membrane stability index; NBT: nitro blue tetrazolium; PPO: polyphenol oxidase; POX: peroxidase (EC. 1.11.17); RH: relative humidity; ROS: reactive oxygen species; RWC: relative water content; SO: Sonalika; SOD: superoxide dismutase (EC 1.15.1.1); TBA: thiobarbituric acid; TCA: trichloroacetic acid.Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H 2 O 2 accumulation

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“…These metabolic changes in plants have also occurred due to progressive water deficit conditions accompanied by lower leaf water potentials [18]. Water stress induces metabolic changes and accumulation of metabolites including proline, ascorbic acid, glutathione, phenolics, and detoxifying enzymes in cereals [19] [20]. Bowne et al (2012) studied metabolic responses in wheat grown under contrasting water levels (drought and irrigated conditions) and reported that amino acids, most notably proline, tryptophan, and the branched chain amino acids, including leu- cine, isoleucine, and valine exhibited higher levels of expression in the leaves of drought grown plants [21].…”

Section: Introductionmentioning

confidence: 99%

LC-HRMS Based Non-Targeted Metabolomic Profiling of Wheat (<I>Triticum aestivum</I> L.) under Post-Anthesis Drought Stress

Rahman¹,

Akond²,

Babar³

et al. 2017

AJPS

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Drought stress at the reproductive stage causes severe damage to productivity of wheat. However, little is known about the metabolites associated with drought tolerance. The objectives of this study were to elucidate changes in metabolite levels in wheat under drought, and to identify potential metabolites associated with drought stress through untargeted metabolomic profiling using a liquid chromatography-high resolution mass spectrometry (LC-HRMS)-based technique called Isotopic Ratio Outlier Analysis. Metabolomic analysis was performed on flag leaves of drought-stressed and control (well-watered) plants after 18 days of post-anthesis drought stress at three-hour intervals over a 24-hour period. Out of 723 peaks detected in leaves, 221 were identified as known metabolites. Sixty known metabolites were identified as important metabolites by 3 different methods, PLS-DA, RF and SAM. The most pronounced accumulation due to drought stress was demonstrated by tryptophan, proline, pipecolate and linamarin, whereas the most pronounced decrease was demonstrated by serine, trehalose, N-acetyl-glutamic acid, DIBOA-glucoside etc. Three different patterns of metabolite accumulation were observed over 24-hour period. The increased accumulated metabolites remained higher during all 8 time points in drought stressed leaves. On the contrary, metabolites that showed decreased level remained significantly lower during all or the most time points. However, the levels of some decreased metabolites were lower during the day, but higher during night in drought stressed leaves. Both univariate and multivariate analyses predicted that N-acetyl-glutamic acid, proline, pipecolate, linamarin, tryptophan, and DIBOA-glucoside could be potential metabolite biomarkers, and their levels could serve as indicators of drought tolerance in wheat.

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Drought stress-induced oxidative stress and antioxidative responses in four wheat (Triticum aestivumL.) varieties

Cited by 49 publications

References 39 publications

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation

LC-HRMS Based Non-Targeted Metabolomic Profiling of Wheat (<I>Triticum aestivum</I> L.) under Post-Anthesis Drought Stress

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Drought stress-induced oxidative stress and antioxidative responses in four wheat (Triticum aestivumL.) varieties

Cited by 49 publications

References 39 publications

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Temperature Stress Induced Antioxidative and Biochemical Changes in Wheat (Triticum aestivum L.) Cultivars

Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation

LC-HRMS Based Non-Targeted Metabolomic Profiling of Wheat (&lt;I&gt;Triticum aestivum&lt;/I&gt; L.) under Post-Anthesis Drought Stress

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LC-HRMS Based Non-Targeted Metabolomic Profiling of Wheat (<I>Triticum aestivum</I> L.) under Post-Anthesis Drought Stress