Drought stress mitigation in Vigna radiata by the application of root-nodulating bacteria

2019

Plant Sci. Today

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Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germination and early seedling stage. In the present study, symbiotic bacteria was isolated and characterised from a collar rot resistant groundnut variety, Mallika and identified as Rhizobium pongamiae following biochemical and molecular characterization (NCBI Acc. No. MK770416). The bacterial isolate on cross inoculation, may be useful in improving crop productivity and enhancing collar rot disease tolerance in susceptible crops.

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of Rhizobium sp. from a collar rot tolerant groundnut (Arachis hypogaea L.) variety

Pandey

2019

Plant Sci. Today

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“…Isolation of bacterial strains, especially, root nodulating bacteria in legumes with plant growth promoting activities assumes significance as it is reported to provide protection to biotic [22] and abiotic stress [23][24][25].…”

Section: Plant Growth Promoting Propertiesmentioning

confidence: 99%

Isolation and Characterization of Plant Growth Promoting Bacteria from Root Nodules of Cicer arientinum L.

Chandra

2023

IJPSS

Aims: The aim of the present study is to isolate and characterize root nodulating bacteria from Cicer arientinum L cv. GNG 1958 and assess its plant growth promoting properties. The study is based on previous reports that native symbiotic bacteria may be replaced by better performing strains by corss-inoculation to increase productivity and tolarance to Biotic and abiotic stresses. Study Design: Isolation of symbiotic bacteria, its biochemical and molecular characterization and assessment of plant growth promoting property through ammonia production, phosphate solubilization and HCN production. Place and Duration of Study: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, during 2019-2022. Methodology: Root nodulating bacterial strain was isolated from C. arientinum L. cv. GNG 1958 grown at the agricultural fields' located at the Krishi Vigyan Kendra, Banasthali Vidypapith, Rajasthan. Morphological characteristics of the isolates were noted following Bergey’s Manual of Systemic Bacteriology and biochemical tests performed as per standard methods. Molecular characterization of the potential symbiont was performed by PCR of 16s rRNA genes and the PCR product was sequenced. Plant growth promoting property was determined through ammonia production, phosphate solubilization and HCN production. Results: Root nodulating bacterial strain was isolated from C. arientinum L.cv. GNG 1958. Out of eighteen isolates, BVCA-58 grew fast and changed the color of BTB dye in media from green to yellow and deep yellow within 2 days of incubation. White colonies were observed when BVCA-58 was inoculated on YEMA media supplemented with Congo red. BVCA-58 has the ability to produce hydrogen cyanide, ammonia and solubilize phosphate. Molecular study of BVCA-58 using 16f-16r and 63f-1244r primers showed 99.89% and 99.62% of similarity with Mesorhizobium jarvisii ATCC-33669 and Mesorhizobium erdmanii USDA-3471 respectively on performing NCBI Blast. BVCA-58 was submitted to NCBI portal as Mesorhizobium sp. strain BVCA-58 with accession nos. OP646813 and OP646810. Conclusion: The present study revealed that isolated strain from Cicer arientinum GNG 1958 variety was Mesorhizobium sp. which has potent plant growth promoting traits.

“…Mungbean, popularly known as Golden Bean, is a significant legume crop from the nutritional viewpoint grown in tropical and subtropical Asia especially for the vegetarian population (11). In India, it is the third most important crop that contains 59.9% carbohydrate, 24.5% protein and 3% vitamins (12).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mungbean [Vigna radiata (L.) Wilczek] varieties under water deficit stress

Kumari

2019

Plant Sci. Today

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Legumes are the most important source of food and fodder but due to climatic changes and global warming; crops are consistently exposed to environmental stresses such as low water shortage, high salinity, mineral toxicity and deficiency, extreme temperatures, etc. Drought is undoubtedly the major constraint limiting plant growth and crop productivity worldwide. The present study was conducted to assess the effect of drought and its physiological responses on the growth of plant and productivity in three different mungbean varieties [Vigna radiata (L.) Wilczek] i.e. IPM 02-3, RMG 975 and IPM 02-14. The studied varieties show significant variation in plant height, root length, pod length, pods/plant, fresh weight of shoot, root and nodule, dry weight of shoot, root and nodule, number of leaves/plant, plant leaf area, relative leaf water content (RLWC) and initiation day of flowering. The effect of drought can better seen at the flowering stage and pod development stage that ultimately reduces crop productivity. The deleterious effect of drought was more prominent in the variety IPM 02-14 in comparison to RMG 975 and IPM 02-3. It can be concluded that varieties IPM 02-3 and RMG 975 are better adapted to drought condition.