-Casssava (Manihot esculenta Crantz) is one of the main food and income sources of about 500 million people in the tropics. The crop is mainly cultivated by small farmers in tropical Africa, Asia and Latin America. Embrapa Mandioca e Fruticultura Tropical, based in Cruz das Almas, Bahia, maintains one of the largest cassava genebanks of Latin America. Among the accessions it contains, those with yellow-orange root color are particularly interesting. The objective of this study was to characterize 30 cassava accessions with yellow-orange root color by RAPD markers. The genetic distances of the 47 analyzed primers varied from 9.0 to 31.7 %, demonstrating the existing genetic variability to be exploited for the development of cassava varieties with higher beta-carotene contents.Molecular characterization of Cassava ( Molecular characterization of Cassava ( Molecular characterization of Cassava ( Molecular characterization of Cassava ( Molecular characterization of Cassava (Manihot esculenta Crantz Crantz Crantz Crantz Crantz) ) ) ) ) with yellow-orange roots for beta-carotene with yellow-orange roots for beta-carotene with yellow-orange roots for beta-carotene with yellow-orange roots for beta-carotene with yellow-orange roots for beta-carotene improvement improvement improvement improvement improvement In Brazil, 4132 accessions have been collected and are maintained in genebanks across the country (Fukuda 2000). Carotenes (-carotene, b-carotene, lycopene) represent the most multifaceted group of pigments in nature, with colors varying from yellow to red, found in photosynthetic and non-photosynthetic tissues, such as roots, seeds and fruits. Once ingested, b-carotene is transformed, in the liver, into Vitamin A. Vitamin A is a micro-nutrient with functions related to vision, cell differentiation, growth development, reproduction and the immune system. Vitamin A deficiency (VAD) can cause severe diseases, e.g., ocular syndrome, xerophthalmia, and advance to irreversible blindness (Underwood et al. 1999). Although the lack of vitamin A can be prevented, xerophthalmia is still a public health problem in many developing countries (Welch and Graham 2002). In general, staple foods are considered poor sources of micro-nutrients. Cassava genetic breeding may modify this situation, through the exploration of diversity encountered in yellow-orange root cassava accessions (Gregorio 2002, Welch 2002, Bedoya et al. 2003. Among the accessions of the Cassava Genebank of Embrapa Cassava and Tropical Fruits, those with yellow-orange roots, which have only recently become the subject of thorough studies, deserve special attention. The roots of these accessions possibly contain high b-carotene contents. Moreover, it is known that there is sufficient genetic diversity in the cassava genebanks that can be explored for this trait (Iglesias et al. 1997, Carvalho 2000. The inheritance for b-carotene concentration in cassava roots is controlled by few genes, i.e., the levels of b-carotene in cassava varieties can be improved thr...