Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Suzuki, Sakaru; Asai, Yoshihiro; Hamada, Toshio; Mizoguchi, Yasuhiro; Yamada, Takashi; Morisawa, Seiji

doi:10.1159/000250827

Cited by 16 publications

(9 citation statements)

References 7 publications

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“…Guinea pig skin soluble fraction was demonstrated to be a potent carrier protein in a blastoid transformation test of lymphocytes from patients with drug eruptions (Suzuki et al 1978), and the finding in the present study that a mixture without soluble fraction generally shows no suppressive effect on ZI-CL of whole blood supports the potency of guinea pig skin soluble fraction as a carrier compound for drug.…”

Section: Discussionsupporting

confidence: 75%

“…According to the method of Suzuki et al (1978), skin soluble fraction was prepared from male guinea pig weighing 300 to 400 g. Normal skin was removed from the abdominal wall and cut to small pieces in 0.25 M sucrose solution. The cutaneous tissue suspension which was homogenized by Ultra -Turrax (Janke Kunkel, FRG) was centrifuged at 50,000 rpm (150,000 x g) in an ultracentrifuge (Hitachi model 65 P-7, Tokyo).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

Kato

Terada

Kuramoto³

et al. 1983

Tohoku J. Exp. Med.

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Conjugates composed of drug, guinea pig skin soluble fraction, and sera from 50 patients with various types of drug eruptions were prepared by the incubation of these mixtures for 60 min at 37°C . And the effects of conjugates on zymosan-induced chemiluminescence (ZI-CL) in whole blood were examined. The conjugates obtained from 32 cases significantly suppressed ZI-CL of whole blood. The mean value of peak intensities of CL in the presence of their conjugates showed 62% of those after the pretreatment of mixtures consisting of drug, soluble fraction and sera from healthy donors. In the systems of non-opsonized and opsonized zymosan-induced CL of leukocyte-erythrocyte sediment, similar inhibitory effects were obtained in both systems in the presence of conjugates, but the time course of CL response was slower in the system with non-opsonized zymosan. In other experiments with the use of immune complexes, which were formed by human albumin and antihuman albumin goat serum in vitro, soluble immune complexes suppressed ZI-Cl of whole blood. These results suggest that immune complexes may be formed during incubation of conjugates and block the receptors on the surfaces of granulocytes, resulting in the suppression of ZI-CL. chemiluminescence of whole blood ; granulocytes ; drug eruptions ; immune complexes

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Section: Discussionsupporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

Kato

Terada

Kuramoto³

et al. 1983

Tohoku J. Exp. Med.

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“…The test was regarded as positive if the stimulation index (SI) was greater than 200. This is slightly higher than in the original publication by Suzuki et al (13).…”

Section: It Investigationscontrasting

confidence: 54%

“…For many years, there has been considerable interest in the development of in vitro immunological methods for the diagnosis of drug allergy (1)(2)(3)12). Most attention has focused on the LIT (4,5,10,13,14). So far, only 72% of patients with proven drug allergy have been found to respond positively to the drug with the LIT (11).…”

Section: Discussionmentioning

confidence: 99%

The Lymphocyte Transformation Test with Sulphadiminidum and with Prealbumin‐Sulphadiminidum Complex in Patients with Drug Allergy

Husz

Kenderessy

Kiss

et al. 1994

The Journal of Dermatology

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Earlier results (the absence or a decreased level of prealbumin [PA] in the serum, a higher proportion of PA-bearing lymphocytes as antigen-binding cells, and the presence of PA in the eluate of lymphocytes in patients with drug allergy) permitted the conclusion that, besides its other relevant functions, PA may also behave as a hapten carrier protein. The lymphocyte transformation test (LTT) involving measurement of 3H-thymidine uptake was therefore carried out with sulphadiminidum (SA) alone, with the PA-SA complex, and with the albumin-SA complex in patients with proven (3 cases) or suspected (63 cases) sensitivity to SA. In all 3 cases with proven drug allergy to SA and in 5 of the 63 suspect cases, the LTT was positive in response to both the drug alone and to the PA-SA complex (The albumin-SA complex gave positivity in only one case). In 5 of the 63 suspect cases, the LTT was positive only in response to the PA-SA complex. It is suggested that the sensitivity of the LTT could be increased if the test could be run with a complex of a protein (hapten carrier?) and a drug.

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“…The uptake of 3H-TdR by lymphocytes was counted by a scintillation counter, and the intensity was expressed as the stimulation index (SI% = cpm observed in stimulated cells/cpm observed in unstimulated cells X 100). From analyses per formed in normal subjects, we have found that an SI of more than 200% is a positive result, while an SI between 180 and 199% is potentially positive [10]. In Japan, the reliability of the LST for estimating drug hypersensitivity has been well discussed.…”

Section: Lymphocyte Stimulation Testmentioning

confidence: 95%

Drug-Induced Hypersensitivity Nephritis: Lymphocyte Stimulation Testing and Renal Biopsy in 10 Cases

Joh¹,

Aizawa²,

Yamaguchi³

et al. 1990

Am J Nephrol

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The pathomorphological and clinical findings were investigated in 10 cases of drug-induced hypersensitivity nephritis. Hypersensitivity due to drugs was strongly suggested by the lymphocyte stimulation test in all patients. The offending drugs included penicillin, cephem derivatives, nonsteroidal anti-inflammatory drugs, and minocycline. All patients developed acute renal failure shortly after administration of regular doses of the drugs. Allergic symptoms plus a raised level of serum IgE or eosinophilia were seen in 7 patients. The remaining 3 patients receiving nonsteroidal anti-inflammatory drugs had no allergic symptoms, but developed severe proteinuria. Eight patients without severe glomerular damage recovered after withdrawal of the offending drugs and temporal dialysis and/or steroid therapy. Renal biopsies revealed tubulitis and tubular epithelial degeneration with interstitial edema as the common characteristic findings. Granulomatous lesions were occasionally observed. Multinucleated giant cells found in the granulomas were positive for LN-3 which is compatible with HLA-DR antigen. The glomeruli appeared normal, except in 2 cases in whom crescentic glomerulonephritis and thrombotic microangiopathy were seen. Our study suggests that the lymphocyte stimulation test and renal biopsy are the most useful means to confirm the diagnosis and provides further evidence for the participation of cell-mediated immunity in the pathogenesis of drug-induced hypersensitivity nephritis.

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Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Cited by 16 publications

References 7 publications

Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

The Lymphocyte Transformation Test with Sulphadiminidum and with Prealbumin‐Sulphadiminidum Complex in Patients with Drug Allergy

Drug-Induced Hypersensitivity Nephritis: Lymphocyte Stimulation Testing and Renal Biopsy in 10 Cases

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