Drug‐Loaded Elastin‐Like Polypeptide–Collagen Hydrogels with High Modulus for Bone Tissue Engineering

Abstract

Back Cover: Doxycycline and rhBMP‐2 loaded collagen‐elastin‐like polypeptide hydrogel is developed with high elastic modulus and anti‐microbial properties. The hydrogel has interconnected, macroporous, three‐dimensional open porous architecture. This architecture supported human adipose‐derived stem cell attachment and proliferation evidenced through rhodamine/DAPI and live/dead staining. Mineralization is observed as bright red stained particles and indicated the terminal osteogenic differentiation of cells. This is reported by Pallabi Pal, Quynh C. Nguyen, Angela H. Benton, Mary E. Marquart, and Amol V. Janorkar in article 1900142.

Functionalized Collagen/Elastin‐like Polypeptide Hydrogels for Craniofacial Bone Regeneration

“…[ 31,32 ] It can be assumed that doxycycline and BMP‐2 would maintain a similar release profile in the current study, where the only difference in the hydrogel composition was the presence of Bioglass. This assumption is based on our observation that the SEM images in the present study (Figure 1) showed average pore diameters in both the hydrogels containing Bioglass ranged from 100 – 350 µm, which are nearly the same as shown in our previous study [ 17 ] for the ELP‐collagen hydrogels that did not contain any Bioglass. While our study was not specifically designed to determine this effect, the presence of doxycycline in our hydrogel is also beneficial in fighting post‐surgical infections and inducing bone formation.…”

“…We have previously shown that the incorporation of the high molecular weight BMP‐2 stiffened the ELP‐collagen hydrogels, likely by reinforcing intra‐ and inter‐molecular hydrogen bonding with collagen‐ELP polymeric chains. [ 17 ]…”

“…Our previous study showed that ≈50% doxycycline was released from the ELP‐collagen hydrogels within the first 24 h while only 10% BMP‐2 was released over a period of one week. [ 17 ] This doxycycline release imparted an effective anti‐microbial effect against three bacterial strains namely, P. Aeruginosa, S. sanguinis , and E. coli . [ 17 ] Nordström et al.…”

“…[ 17 ] This doxycycline release imparted an effective anti‐microbial effect against three bacterial strains namely, P. Aeruginosa, S. sanguinis , and E. coli . [ 17 ] Nordström et al. and Smith et al.…”

“…In our previous in vitro study, we demonstrated that BMP‐2 and doxycycline‐loaded non‐crosslinked collagen‐ELP composites exhibited tunable release, and imparted anti‐microbial and mechanical properties appropriate for osteogenesis. [ 17 ] Bioactive glasses have been used as bone graft materials for bone defect repair and regeneration because of their bioactive and osteoconductive properties. For example, more than a million patients have benefitted from using 45S5 Bioglass for bone defect repair within orthopedics and maxillofacial surgery.…”