Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp

Almeida, Marília Viana Albuquerque de; Cangussú, Ítalo Mendes; Carvalho, Antonia Leonádia Siqueira de; Brito, Izabelly Linhares Ponte; Costa, Renata Albuquerque

doi:10.1590/s1678-9946201759070

Cited by 26 publications

(16 citation statements)

References 40 publications

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“…In this study, 30% of the isolates were resistant to this antibiotic. However, de Almeida et al [34] reported a single isolate resistant to Ceftriaxone and Verner-Jeffreys et al [35] reported all the isolates susceptible to it, which are contrasting to current findings. In this study, 9.6% isolates were resistant to trimethoprim-sulfamethoxazole, a bactericidal drug which works by inhibiting folic acid synthesis.…”

Section: Biochemical Identificationcontrasting

confidence: 89%

Frequency and antibiotic sensitivity of gram negative bacteria isolated from raw fish sold in Karachi, Pakistan

Mansoor

Naim

Naeem

2019

PAB

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Fish serve as an important source of nutrition and also acts as a reservoir for different pathogenic organisms. Sewage pollution in the water bodies is the major source of pathogenic bacteria in seafood. In this study, the presence of Gram negative bacteria in raw fish samples was detected and the antibiotic susceptibility pattern of the isolates was determined.A total of 25 raw fish samples were collected at random from different local markets of Karachi in sterile zip-lock plastic bags. From these samples, a total of 73 Gram negative bacterial strains were isolated. Of these 73 Gram negative isolates, 6 were E.

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Section: Biochemical Identificationcontrasting

confidence: 89%

Frequency and antibiotic sensitivity of gram negative bacteria isolated from raw fish sold in Karachi, Pakistan

Mansoor

Naim

Naeem

2019

PAB

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“…Several studies revealed a high prevalence of non-susceptibility to this class of antibiotic in fish, shrimps and water samples from aquaculture farms. These studies reported the discovery of bla SHV and bla TEM genes, associated with β-lactam non-susceptibility [ 91 , 92 , 93 , 94 ]. In our study, β-lactam resistance genes were not detected.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

et al. 2020

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In a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of “critically important antimicrobials” and “highly important antimicrobials” for human medicine.

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“…Biochemistry has increased the number of β-lactamase enzymes with enlarged substrate specificities [ 129 ]. The substrate range includes cephalosporins (cefotaxime, ceftriaxone [ 130 , 131 , 132 ]), monobactam aztreonam [ 133 , 134 , 135 ], amino-penicillin combinations [ 136 ], lactamase inhibitor ampicillin-sulbactam [ 137 , 138 , 139 , 140 ], ureido-penicillins [ 141 , 142 ] including piperacillin/tazobactam [ 143 , 144 , 145 , 146 ], temocillin [ 147 ], piperacillin/tazobactam [ 148 ], and ceftolozane-tazobactam [ 149 ]. Furthermore, the appearance of new enzymes is not associated with the loss of ability to hydrolyze the earlier lactams, such as ampicillin [ 36 ].…”

Section: Resistance Problems Related To Gram-negative Pathogensmentioning

confidence: 99%

Multidrug Resistance (MDR) and Collateral Sensitivity in Bacteria, with Special Attention to Genetic and Evolutionary Aspects and to the Perspectives of Antimicrobial Peptides—A Review

Fodor

Abate²,

Deák

et al. 2020

Pathogens

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Antibiotic poly-resistance (multidrug-, extreme-, and pan-drug resistance) is controlled by adaptive evolution. Darwinian and Lamarckian interpretations of resistance evolution are discussed. Arguments for, and against, pessimistic forecasts on a fatal “post-antibiotic era” are evaluated. In commensal niches, the appearance of a new antibiotic resistance often reduces fitness, but compensatory mutations may counteract this tendency. The appearance of new antibiotic resistance is frequently accompanied by a collateral sensitivity to other resistances. Organisms with an expanding open pan-genome, such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae, can withstand an increased number of resistances by exploiting their evolutionary plasticity and disseminating clonally or poly-clonally. Multidrug-resistant pathogen clones can become predominant under antibiotic stress conditions but, under the influence of negative frequency-dependent selection, are prevented from rising to dominance in a population in a commensal niche. Antimicrobial peptides have a great potential to combat multidrug resistance, since antibiotic-resistant bacteria have shown a high frequency of collateral sensitivity to antimicrobial peptides. In addition, the mobility patterns of antibiotic resistance, and antimicrobial peptide resistance, genes are completely different. The integron trade in commensal niches is fortunately limited by the species-specificity of resistance genes. Hence, we theorize that the suggested post-antibiotic era has not yet come, and indeed might never come.

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Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp

Cited by 26 publications

References 40 publications

Frequency and antibiotic sensitivity of gram negative bacteria isolated from raw fish sold in Karachi, Pakistan

Frequency and antibiotic sensitivity of gram negative bacteria isolated from raw fish sold in Karachi, Pakistan

Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

Multidrug Resistance (MDR) and Collateral Sensitivity in Bacteria, with Special Attention to Genetic and Evolutionary Aspects and to the Perspectives of Antimicrobial Peptides—A Review

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