Drug resistance and virulence traits of Acinetobacter baumannii from Turkey and chicken raw meat

Kanaan, Manal Hadi Ghaffoori; Al-Shadeedi, Shahrazad M.; Al-Massody, Aseel Jameel; Ghasemian, Abdolmajid

doi:10.1016/j.cimid.2020.101451

Cited by 34 publications

(20 citation statements)

References 22 publications

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“…Resistance against diaminopyrimidines in A. baumannii infections is mainly conferred by trimethoprim-resistant dihydrofolate reductases DfrA1, DfrA5, DfrA7, DfrA10, DfrA12, DfrA14, DfrA16, DfrA17, DfrA19, DfrA20, DfrA27, and DfrB1 (encoded by dfrA1, dfrA5, dfrA7, dfrA10, dfrA12, dfrA14, dfrA16, dfrA17, dfrA19, dfrA20, dfrA27, and dfrB1, respectively) [97]. A recent study of A. baumannii in turkey and chicken raw meat revealed that prevalence of dfrA was as high as 71% [186]. The latter antibiotic resistance gene seems to be a typical part of cassettes belonging to class I integron [187].…”

Section: Resistance To Diaminopyrimidines-sulfonamidesmentioning

confidence: 99%

Acinetobacter baumannii Antibiotic Resistance Mechanisms

et al. 2021

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Acinetobacter baumannii is a Gram-negative ESKAPE microorganism that poses a threat to public health by causing severe and invasive (mostly nosocomial) infections linked with high mortality rates. During the last years, this pathogen displayed multidrug resistance (MDR), mainly due to extensive antibiotic abuse and poor stewardship. MDR isolates are associated with medical history of long hospitalization stays, presence of catheters, and mechanical ventilation, while immunocompromised and severely ill hosts predispose to invasive infections. Next-generation sequencing techniques have revolutionized diagnosis of severe A. baumannii infections, contributing to timely diagnosis and personalized therapeutic regimens according to the identification of the respective resistance genes. The aim of this review is to describe in detail all current knowledge on the genetic background of A. baumannii resistance mechanisms in humans as regards beta-lactams (penicillins, cephalosporins, carbapenems, monobactams, and beta-lactamase inhibitors), aminoglycosides, tetracyclines, fluoroquinolones, macrolides, lincosamides, streptogramin antibiotics, polymyxins, and others (amphenicols, oxazolidinones, rifamycins, fosfomycin, diaminopyrimidines, sulfonamides, glycopeptide, and lipopeptide antibiotics). Mechanisms of antimicrobial resistance refer mainly to regulation of antibiotic transportation through bacterial membranes, alteration of the antibiotic target site, and enzymatic modifications resulting in antibiotic neutralization. Virulence factors that may affect antibiotic susceptibility profiles and confer drug resistance are also being discussed. Reports from cases of A. baumannii coinfection with SARS-CoV-2 during the COVID-19 pandemic in terms of resistance profiles and MDR genes have been investigated.

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Section: Resistance To Diaminopyrimidines-sulfonamidesmentioning

confidence: 99%

Acinetobacter baumannii Antibiotic Resistance Mechanisms

et al. 2021

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“…Furthermore, numerous bacteria secrete toxins and extracellular enzymes which play a crucial role in the apoptosis or necrosis of epithelial cells or immunocytes. Various virulence factors of A. baumannii such as adhesins genes like kpsMII (group 2 capsule synthesis) and fimH , tratT (serum resistance associated), fyuA (yersiniabactin receptor) and iutA (aerobactin receptor) have been investigated previously 9 , 10 . An important polysaccharide for biofilm formation is encoded by pgaABCD locus 11 .…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Hetta

Al-Kadmy

Khazaal

et al. 2021

Sci Rep

105

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We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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“…Acinetobacter is widely considered the primary microorganism in poultry processing environment (Kanaan et al, 2020;Wu et al, 2017). It has strong adhesion capabilities and may serve as a source of bacterial degradation during storage.…”

Section: Discussionmentioning

confidence: 99%

Investigation of microbial contamination in a chicken slaughterhouse environment

Song

Wang

2021

Journal of Food Science

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The environment in poultry abattoirs is the primary potential source of bacterial contamination and cross-contamination of broiler carcasses. In this context, we explored the influence of chilling water and contact surfaces on the microbial diversity of broiler carcasses in warm and cold seasons. Highthroughput sequencing was used to target the V3-V4 region of the 16S rRNA gene.Proteobacteria was the main phylum detected in broiler carcasses and on contact surfaces, whereas Bacteroidetes and Firmicutes had high abundances of the prechilling water in both seasons. At the genus level, Psychrobacter and Acinetobacter were much more abundant on broiler carcasses in the warm season, while Flavobacterium and Psychrobacter dominated in the cold season. LEfSe analysis showed that the chilling tank was a key location where carcass contamination occurred. Therefore, the risk of carcass contamination can be reduced by improving sanitary conditions during processing, installing longer chilling tanks, or increasing the water exchange rate in chilling tanks. The results of this study may be useful for better slaughterhouse environmental hygiene management in different seasons. Practical Application: This study will help poultry processing managers better understand the impact of different seasons on the environmental microbiota in the environment and their abundance in poultry processing plants, thus allowing them to adopt proper disinfection strategies for different seasons and environments, further improving the safety and shelf life of products.

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Drug resistance and virulence traits of Acinetobacter baumannii from Turkey and chicken raw meat

Cited by 34 publications

References 22 publications

Acinetobacter baumannii Antibiotic Resistance Mechanisms

Acinetobacter baumannii Antibiotic Resistance Mechanisms

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Investigation of microbial contamination in a chicken slaughterhouse environment

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