Drug resistance detection and mutation patterns of multidrug resistant tuberculosis strains from children in Delhi

Arora, Jyoti; Singhal, Ritu; Bhalla, Manpreet; Verma, Ajoy Kumar; Singh, Namit; Behera, Digamber; Sarin, Rohit; Myneedu, Vithal Prasad

doi:10.1016/j.jegh.2016.12.003

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…The prevalence of childhood TB with resistant strains is indicative of household contact [ 11 ], thus making screening of children essential in limiting the pediatric spread of TB. Moreover, for children, other sampling techniques need to be standardized as young children may not be able to expectorate [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Saudi Arabian Public Health Perspective of Tuberculosis

Saati

Khurram

Faidah

et al. 2021

IJERPH

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Tuberculosis is a global health challenge due to its spreading potential. The Kingdom of Saudi Arabia (KSA) faces a challenge in the spread of tuberculosis from migrant workers, but the foremost threat is the huge number of pilgrims who travel to visit sacred sites of the Islamic world located in the holy cities of Makkah and Al Madina. Pilgrims visit throughout the year but especially in the months of Ramadan and Zul-Hijah. The rise of resistance in Mycobacterium tuberculosis is an established global phenomenon that makes such large congregations likely hotspots in the dissemination and spread of disease at a global level. Although very stringent and effective measures exist, the threat remains due to the ever-changing dynamics of this highly pathogenic disease. This overview primarily highlights the current public health challenges posed by this disease to the Saudi health system, which needs to be highlighted not only to the concerned authorities of KSA, but also to the concerned global quarters since the pilgrims and migrants come from all parts of the world with a majority coming from high tuberculosis-burdened countries.

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Section: Introductionmentioning

confidence: 99%

A Saudi Arabian Public Health Perspective of Tuberculosis

Saati

Khurram

Faidah

et al. 2021

IJERPH

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“…19 A study from Delhi among children with a presumptive diagnosis of multidrug-resistant (MDR) TB, 61.8% of the sputum samples were smear-positive by ZN staining and 92.8% were positive by LPA. 20 The higher sputum smear and LPA positive results of this study, when compared to ours, was not surprising because their study population was already those with a presumptive MDR-TB. All the above three studies [18][19][20] used LPA to identify MTB drug resistance, whereas, in the present study, we used LPA as a tool for detecting MTB to improve the diagnosis of PTB in children.…”

Section: Discussionmentioning

confidence: 39%

Improving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria

Ebonyi

Oguche

Abok

et al. 2020

Germs

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Introduction Diagnosing tuberculosis (TB), including pulmonary tuberculosis (PTB), in children remains a challenge, partly due to its paucibacillary nature in young children. Data on the use of line probe assay (LPA), on gastric and sputum samples, for diagnosing PTB in children are scarce. We determined the proportion of samples positive for Mycobacterium tuberculosis (MTB) by smear microscopy (SM) and LPA in presumptive PTB cases as well as the factors associated with PTB confirmed by LPA, in children in Jos, Nigeria. Methods An observational study in children aged 6 months-16 years. Gastric and sputum samples were examined by SM and by LPA for MTB using GenoType MTBDRplus Ver 2.0 (Hain Lifescience). Multivariate logistic regression was performed to determine the factors associated with PTB. Results Out of 103 children with presumptive PTB, 47 had confirmed PTB, 26 unconfirmed PTB and 30 unlikely PTB by LPA. In 67 gastric samples, MTB was identified by SM in 2 (3.0%) compared to 28 (41.8%) by LPA while in 31 sputum samples, MTB was identified by SM in 5 (16.1%) compared to 18 (58.1%) by LPA. The factors associated with pulmonary tuberculosis were an abnormal chest X-ray (adjusted odds ratio (AOR))=12.39 [3.75-40.90], p<0.001), sleeping in the same room with more than three persons (AOR=3.30 [1.23-8.85], p=0.018) and sleeping in a room with none or one window (AOR=2.86 [1.03-7.95], p=0.044). Conclusions Line probe assay improves the diagnosis of pulmonary TB in children, especially with gastric samples, while an abnormal chest X-ray is a useful adjunct in PTB diagnosis. Avoiding overcrowding and having windows in sleeping rooms are a necessary part of TB prevention.

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“…“Our studies have found that its incidence among children in Mumbai has increased from 5% in 2007-2010 to 10% in 2013-2016, and continues to grow.”2…”

Section: Nine Countriesmentioning

confidence: 99%