Drug Resistance Mechanism of Enterobacteriaceae with Decreased Antibiotic Sensitivity

Abstract

To investigate the mechanism of antibiotic resistance in Enterobacteriaceae. Carbapenem Enterobacteriaceae bacteria isolated from a hospital from January 2015 to June 2020 were selected. Drug resistance phenotype test, drug sensitivity test, and conjugation test were used to observe the drug sensitivity results and the titer of acrB antibody. Finally, the data were statistically analyzed. All strains were resistant to ceftazidime, ceftriaxone, ertapenem, and aztreonam. 87.5% of the samples were resistant to piperacillin. Multisite sequence typing showed that 5 carbapenem-resistant Klebsiella pneumoniae belonged to 4 different types. The sequence types of kpn6099 and kpn6617 were the same. In the sensitivity comparison, Escherichia coli J53 was more sensitive to these two antibiotics, minimum inhibitory concentration values were 0.5 and 0.25 μg/ml, respectively. In addition, the sensitivity of E. coli J53 to carbapenems was slightly higher than that of kpn6617. The results showed that the enzyme-linked immunosorbent assay titer of acrB antibody was 1:40,000, and the preparation of acrB antibody was successful. Plasmid-mediated deletion of IMP-1 metallo-β-lactamase binding outer membrane protein is one of the main reasons for the decrease of antibiotic sensitivity.

Prevalence and Antibiotic Resistance Pattern of <em>Streptococcus, Staphylococcus, Neisseria meningitidis and Enterobacteriaceae</em> in Two Reference Hospitals of Yaoundé: An Overview before and during COVID-19 Pandemic Era

“…This result is not different from that of Sanofi, which estimates that 30% of Klebsiella pneumoniae strains show resistance to 3rd generation cephalosporins in France, in 2020 [12]. Unlike Neisseria meningetidis, Enterobacteriaceae are widely distributed and have a large host range [24], they can cross-infect and spread between medical staff and patients, and also their genetic materials (such as plasmids or transposons) can be obtained from the outside world, leading to horizontal transmission of drug-resistant genes, which further leads to the wide spread of drug-resistant bacteria [25,26].…”

Antibiotic Resistance Trends Among Enterobacteriaceae in Saudi Arabia: A Systematic Review

“…This can lead to a reduction in the effectiveness of antibiotics against infectious pathogens. Furthermore, the irrational use of antibiotics has resulted in many pathogens developing multidrug resistance, which is a cause of great concern in the medical community [47]. In recent years, the widespread utilization of βlactamases has resulted in the elimination of the effectiveness of most cephalosporins against Enterobacteriaceae.…”

Retracted: Drug Resistance Mechanism of Enterobacteriaceae with Decreased Antibiotic Sensitivity

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”