Drug sensitivity of trypanosome populations from cattle in a peri-urban dairy production system in Uganda

Olila, D.; McDermott, JJ; Eisler, M.; Mitema, Eric S.; Patzelt, R.J.; Clausen, P.H.; Poetzsch, CJ; Zessin, Karl-Hans; Mehlitz, D; Peregrine, A. S.

doi:10.1016/s0001-706x(02)00137-7

Cited by 9 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the treatment regime used is consistent with previous results on the curative properties of diminazene aceturate in the treatment trypanosomiasis in cattle and other species (Münstermann et al,1992;Sirivan et al,1994;Olila et al, 2002 ;Da Silva et al, 2009). Packed cell volume (as a measure of anaemia) has been used to determine severity of trypanosomosis (Katunguka-Rwakishaya, 1999).…”

Section: Post-infection Time (Weeks)supporting

confidence: 90%

“…(Taylor, 1998 ;Chaka and Abebe, 2003;Camargo et al, 2004). DA alongside Isomethamidium chloride and Homidium bromide have been reported to be the most widely used drugs against trypanosomiasis in cattle and other species (Kinabo, 1993;Perengrine et al 1993;Chartier et al, 2000;Van den Bossche et al, 2000: Olila et al, 2002Chaka and Abebe, 2003) Vitamin B 12 (cyanocobalamin and hydroxocobalamin) is a water soluble vitamin and plays an important role in erythrocyte synthesis as well as metabolism of fatty acids and folic acid (Reginald et al, 2000;Moulin and Coquerel, 2002). Cognisant of the significant anaemic effect the disease, pharmaceutical companies turn to associate vitamins especially vitamin B 12 to DA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of co-administration of vitamin B12 with diminazene aceturate on packed cell volume and weight gain in cattle experimentally infected with <i>Trypanosoma congolense</i>

Fon-tebug¹,

Maganga²,

Gbati³

et al. 2010

International Journal of Biological and Chemical Sciences

View full text Add to dashboard Cite

The effects of co-administration of cyanocobalamin and/or hydroxocobalamin with diminazene aceturate (DA) on, pack cell volume and weight gain in cattle experimentally infected with Trypanosoma congolense was studied. Twenty eight young zebu bulls aged 10-16 months with an average weight of 92.02 ± 14.74 kg were randomly distributed into 4 groups. These bulls were infected with Trypanosoma congolense intravenously at a dose rate of 1×10 5 suspended in 4 ml of phosphate buffered saline per animal. Each group was treated with a commercial medication containing DA ,cyanocobalamin and/or hydroxocobalamin 10 days post-infection. Haematological examination showed no trypanosomes irrespective of the regimen administered 48 hours post-treatment in all infected cattle. Packed Cell Volume (PCV) and weight gain was highest with the regimen containing DA, cyanocobalamin and hydroxocobalamin. The trypanocidal regimens containing DA co-administered with cyanocobalamin and/or hydroxocobalamin enabled a rapid reconstitution of red blood cells and led to improvement in the weight gain of the trypanosome-infected cattle.

show abstract

Section: Post-infection Time (Weeks)supporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effects of co-administration of vitamin B12 with diminazene aceturate on packed cell volume and weight gain in cattle experimentally infected with <i>Trypanosoma congolense</i>

Fon-tebug¹,

Maganga²,

Gbati³

et al. 2010

International Journal of Biological and Chemical Sciences

View full text Add to dashboard Cite

show abstract

“…Furthermore, in the field, cross-resistance is observed between ethidium bromide and ISM [12] suggesting that uptake and extrusion of the drug within and from the trypanosome are mediated by the same mechanisms for both compounds.…”

Section: Introductionmentioning

confidence: 99%

Chemosensitization of Trypanosoma congolense Strains Resistant to Isometamidium Chloride by Tetracyclines and Enrofloxacin

et al. 2010

View full text Add to dashboard Cite

BackgroundBecause of the development of resistance in trypanosomes to trypanocidal drugs, the livelihood of millions of livestock keepers in sub-Saharan Africa is threatened now more than ever. The existing compounds have become virtually useless and pharmaceutical companies are not keen on investing in the development of new trypanocides. We may have found a breakthrough in the treatment of resistant trypanosomal infections, through the combination of the trypanocide isometamidium chloride (ISM) with two affordable veterinary antibiotics.Methodology/Principal FindingsIn a first experiment, groups of mice were inoculated with Trypanosoma congolense strains resistant to ISM and either left untreated or treated with (i) tetracycline, (ii) ISM or (iii) the combination of the antibiotic and the trypanocide. Survival analysis showed that there was a significant effect of treatment and resistance to treatment on the survival time. The groups treated with ISM (with or without antibiotic) survived significantly longer than the groups that were not treated with ISM (P<0.01). The group treated with the combination trypanocide/antibiotic survived significantly longer than the group treated with ISM (P<0.01). In a second experiment, groups of cattle were inoculated with the same resistant trypanosome strain and treated with (i) ISM, (ii) ISM associated with oxytetracycline or (iii) ISM associated with enrofloxacine. All animals treated with ISM became parasitaemic. In the groups treated with ISM-oxytetracycline and ISM-enrofloxacine, 50% of the animals were cured. Animals from the groups treated with a combination trypanocide/antibiotic presented a significantly longer prepatent period than animals treated with ISM (p<0.001). The impact of the disease on the haematocrit was low in all ISM treated groups. Yet, it was lower in the groups treated with the combination trypanocide/antibiotic (p<0.01).Conclusions/SignificanceAfter optimization of the administration protocol, this new therapeutic combination could constitute a promising treatment for livestock infected with drug resistant T. congolense.

show abstract

“…Glossina fuscipes fuscipes, for example, is considered the key vector for human infection by the parasite T. brucei in southeastern Uganda (Hide, 1999), whereas the tsetse vector, G. pallidipes, may also play a role in sleeping sickness transmission in some regions (Matovu, 1982;Okiria, 1985). In southeastern Uganda, T. brucei is generally not associated with high rates of cattle mortality, thus resulting in a larger reservoir of infected cattle (Olila et al, 2002). The extent to which low-level but persistent infections in cattle increase or decrease the potential for human infection is the subject of current studies .…”

Section: Sleeping Sickness Parasite Life-cycle Systemmentioning

confidence: 99%

“…Mathematical transmission models such as that developed by Rogers (Rogers, 1998), for example, can be incorporated into a GIS to allow for spatial variation of population variables and infection parameters. These analyses can be integrated with scenario-based approaches, such as those used by Reid et al (2000), McDermott et al (2001), andMcDermott and. There has been recent integration of spatial analyses with statistical modeling to study sleeping sickness in Uganda Odiit et al, 2004a).…”

Section: Approaches To Modeling Sleeping Sickness In Southeastern Ugandamentioning

confidence: 99%

Sleeping Sickness in Southeastern Uganda: A SystemsApproach

et al. 2005

View full text Add to dashboard Cite

Sleeping sickness continues to be a significant public health burden in southeastern Uganda.Continued spread of the disease into new areas of Uganda highlights our inability to understand and predict the distribution of infection. Multiple factors influence the distribution of sleeping sickness, including climate, land cover, cattle movements, prevention and control activities, and social conflict. We draw on a systems approach to conceptualize and characterize the multiple interacting forces and processes that influence the spatial and temporal dynamics of sleeping sickness in Uganda. This synthesis reveals a complex system of interactions among human and biophysical systems, feedback, and scale dependence. We identify some common analytical modeling approaches relative to our system characterization and identify opportunities for sleeping sickness research and improved understanding of disease dynamics in Uganda.

show abstract

Drug sensitivity of trypanosome populations from cattle in a peri-urban dairy production system in Uganda

Cited by 9 publications

References 24 publications

Effects of co-administration of vitamin B12 with diminazene aceturate on packed cell volume and weight gain in cattle experimentally infected with <i>Trypanosoma congolense</i>

Effects of co-administration of vitamin B12 with diminazene aceturate on packed cell volume and weight gain in cattle experimentally infected with <i>Trypanosoma congolense</i>

Chemosensitization of Trypanosoma congolense Strains Resistant to Isometamidium Chloride by Tetracyclines and Enrofloxacin

Sleeping Sickness in Southeastern Uganda: A SystemsApproach

Contact Info

Product

Resources

About