Drug susceptibility testing of Mycobacterium tuberculosis by the broth microdilution method with 7H9 broth

Çoban, Ahmet Yılmaz; Bırınci, Asuman; Ekinci, Bora; Durupinar, Belma

doi:10.1590/s0074-02762004000100020

Cited by 27 publications

(23 citation statements)

References 8 publications

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“…One study assessed 7H9 broth microdilution for DST of common anti-TB drugs but did not report the actual MIC values (13). However, similar MIC distributions have been reported for the same reference strain between different liquid culture systems, with MIC variability of a 2-fold dilution or less (5).…”

Section: Discussionmentioning

confidence: 99%

A Multilaboratory, Multicountry Study To Determine MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing of Selected First-Line Antituberculosis Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine, and Linezolid

et al. 2016

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jOur objective was to establish reference MIC quality control (QC) ranges for drug susceptibility testing of antimycobacterials, including first-line agents, second-line injectables, fluoroquinolones, and World Health Organization category 5 drugs for multidrug-resistant tuberculosis using a 7H9 broth microdilution MIC method. A tier-2 reproducibility study was conducted in eight participating laboratories using Clinical Laboratory and Standards Institute (CLSI) guidelines. Three lots of custom-made frozen 96-well polystyrene microtiter plates were used and prepared with 2؋ prediluted drugs in 7H9 broth-oleic acid albumin dextrose catalase. The QC reference strain was Mycobacterium tuberculosis H37Rv. MIC frequency, mode, and geometric mean were calculated for each drug. QC ranges were derived based on predefined, strict CLSI criteria. Any data lying outside CLSI criteria resulted in exclusion of the entire laboratory data set. Data from one laboratory were excluded due to higher MIC values than other laboratories. QC ranges were established for 11 drugs: isoniazid (0.03 to 0.12 g/ml), rifampin (0.03 to 0.25 g/ml), ethambutol (0.25 to 2 g/ml), levofloxacin (0.12 to 1 g/ml), moxifloxacin (0.06 to 0.5 g/ml), ofloxacin (0.25 to 2 g/ml), amikacin (0.25 to 2 g/ml), kanamycin (0.25 to 2 g/ml), capreomycin (0.5 to 4 g/ml), linezolid (0.25 to 2 g/ml), and clofazimine (0.03 to 0.25 g/ml). QC ranges could not be established for nicotinamide (pyrazinamide surrogate), prothionamide, or ethionamide, which were assay nonperformers. Using strict CLSI criteria, QC ranges against the M. tuberculosis H37Rv reference strain were established for the majority of commonly used antituberculosis drugs, with a convenient 7H9 broth microdilution MIC method suitable for use in resource-limited settings. Managing an estimated global annual incidence of 9.6 million Mycobacterium tuberculosis cases requires an arsenal of effective antituberculosis (anti-TB) drugs that are active against both susceptible and multidrug-resistant strains (1). Furthermore, maximizing the effectiveness of treatment and minimizing the risk of development of further resistance requires accurate drug susceptibility testing (DST) for M. tuberculosis clinical isolates (2, 3). To improve reliability of DST for M. tuberculosis, regulators have introduced a requirement to establish quality control (QC) ranges for new and repurposed anti-TB drugs (4).Two approaches to M. tuberculosis DST are currently employed: critical concentration (CC)-based agar proportion assay (APA) and MIC-based methodology. CC is well established and used widely for classifying the sensitivity of M. tuberculosis to anti-TB drugs. The CC, also known as the antimicrobial susceptibility testing breakpoint, is defined by international convention as the lowest concentration of a drug that inhibits growth of 95% (90% for pyrazinamide) of wild-type M. tuberculosis strains, while at the same time it does not inhibit strains that are considered to be clinically resistant (i.e., isolated from patients unres...

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Section: Discussionmentioning

confidence: 99%

A Multilaboratory, Multicountry Study To Determine MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing of Selected First-Line Antituberculosis Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine, and Linezolid

et al. 2016

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“…Detecting tuberculosis and identifying MDR Mycobacterium tuberculosis strains by conventional methods is difficult because of the low growth rate of the causative agent. Therefore, rapid and efficient methods are needed for the control of this disease (3)(4)(5)(6)13).…”

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“…and the BACTEC MGIT 960 (Becton Dickinson), have become available for the susceptibility testing of M. tuberculosis and are also recommended by the NCCLS. Although the time to detect M. tuberculosis from clinical specimens can be shortened and the results for susceptibility testing can be obtained in 4 to 7 days by the use of these systems, they are labor-intensive and expensive and generate radioactive waste (2)(3)(4)12). Therefore, several methods based on liquid media have been developed by different investigators for the susceptibility testing of M. tuberculosis (2, 4-6, 10, 11, 13, 14).…”

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Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar

Çoban¹,

Bılgın²,

Uzun

et al. 2005

J Clin Microbiol

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In this study, blood agar was used instead of 7H10 agar for the susceptibility testing of 34 clinical isolates of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) in accordance with the NCCLS. The BACTEC 460 TB system (Becton Dickinson, Sparks, Md.) was used as a "gold standard." Results for both media were in agreement for RIF and INH at 100 and 94.1%, respectively. For INH, the specificity, sensitivity, positive predictive value, and negative predictive value were found to be 71.4, 100, 93.1, and 100%, respectively, while these values were 100% for RIF. In addition, the results of the susceptibility test performed with blood agar were obtained on day 14 of incubation. In conclusion, results were obtained much earlier with blood agar (2 weeks) than with 7H10 agar (3 weeks), and the results of this study suggest that blood agar may be used as an alternative medium for the susceptibility testing of M. tuberculosis to INH and RIF.The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) produces serious problems in developed and especially in developing countries. Detecting tuberculosis and identifying MDR Mycobacterium tuberculosis strains by conventional methods is difficult because of the low growth rate of the causative agent. Therefore, rapid and efficient methods are needed for the control of this disease (3-6, 13).Several manufacturers have directed considerable effort toward the development of rapid and efficient systems for the growth, detection, and susceptibility testing of mycobacteria. Two of these systems, the BACTEC 460 TB (Becton Dickinson, Sparks, Md.) and the BACTEC MGIT 960 (Becton Dickinson), have become available for the susceptibility testing of M. tuberculosis and are also recommended by the NCCLS. Although the time to detect M. tuberculosis from clinical specimens can be shortened and the results for susceptibility testing can be obtained in 4 to 7 days by the use of these systems, they are labor-intensive and expensive and generate radioactive waste (2-4, 12). Therefore, several methods based on liquid media have been developed by different investigators for the susceptibility testing of M. tuberculosis (2, 4-6, 10, 11, 13, 14).Drancourt et al. (7) investigated the effectiveness of blood agar for primary isolation of M. tuberculosis. They reported that M. tuberculosis can easily grow on blood agar in 1 to 2 weeks and that this medium has been routinely used instead of egg-based medium in the inoculation of 10,000 samples in a year for the diagnosis of tuberculosis, with the same results being obtained.In this study, we evaluated the performance of blood agar for susceptibility testing of 34 M. tuberculosis clinical isolates to isoniazid (INH) and rifampin (RIF) by using the proportion method.Bacterial isolates. Thirty-four clinical isolates of M. tuberculosis were examined in this study, and H37Rv and H37Ra were also included as control strains. Drug susceptibility patterns of all isolates were previously detected by the BACTEC 460 TB system, and a standard prot...

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“…12 Arıların çiçek nektarlarından veya bitkiler üzerinde yaşayan bazı canlıla-rın ürettikleri salgılardan topladıkları ve enzimatik olarak değişikliğe uğratarak meydana getirdikleri bal, besleyiciliğinin yanı sıra insan ve hayvan sağlığında yüzyıllardan beri kullanılan şifalı bir ürünüdür. [13][14][15] Bal; enfeksiyonların çabuk temizlenmesi, yaralardan ölü dokuların ve yabancı maddelerin çabuk uzaklaş-tırılması, inflamasyonun hızlı baskılanması, yara ve yara izinin hızlı azalması, yeni damar oluşumu, doku granülasyonu ve epitelyum gelişmesinin uyarılmasını sağlamaktadır. 13,15,16 Balın antibiyotiğe dirençli bakterilere karşı güçlü in vitro aktivitesine sahip olduğu ve antibiyotik tedavisine yanıt vermeyen kronik yara enfeksiyonlarında antibakteriyel ajan olarak kullanıldığı bildirilmiştir.…”

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Investigation of Antioxidant Capacity and Antimicrobial Potential of Pine Honey from Turkey Geography

Nisbet¹,

Çoban²,

Taştekin³

2018

J Tradit Complem Med

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Drug susceptibility testing of Mycobacterium tuberculosis by the broth microdilution method with 7H9 broth

Abstract: In this study, we have evaluated the broth microdilution method (BMM) 100, 100, 96.9, and 90.2%, while specificity was 100, 85.7, 90.9, and 100% for INH, RIF, STR, and ETM, respectively

Cited by 27 publications

References 8 publications

A Multilaboratory, Multicountry Study To Determine MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing of Selected First-Line Antituberculosis Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine, and Linezolid

A Multilaboratory, Multicountry Study To Determine MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing of Selected First-Line Antituberculosis Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine, and Linezolid

Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar

Investigation of Antioxidant Capacity and Antimicrobial Potential of Pine Honey from Turkey Geography

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