Dry mycelium of Penicillium chrysogenum protects cotton plants against wilt diseases and increases yield under field conditions

Dong, Hezhong; Zhang, Xuekun; Choen, Yigal; Zhou, Yang; Li, Weijiang; Li, Zhenhuai

doi:10.1016/j.cropro.2005.05.003

Cited by 32 publications

(13 citation statements)

References 17 publications

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“…bDHA can also be used as a leading factor in a wide range of activities against many phytopathogens, where these pathogens have developed resistance against the specific fungicides (benzimidazoles, dicarboximides, diethofuncarband and the sterol biosynthesis inhibitors) [7], resulting in serious environmental damage. Among these pathogens, wilt disease of cotton caused by F. oxysporum has been reported to be very destructive to crops under field conditions in China, where about 4 million hectares of cotton, accounting for 20% of the world's total production, is grown annually [5]. In our study, it has become clear that bDHA possesses great potential to strongly inhibit the growth of F. oxysporum along with other plant pathogenic fungi tested.…”

Section: Discussionmentioning

confidence: 89%

Microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bDHA) used against agricultural plant pathogenic fungi

Bajpai

Kim²,

Hou

et al. 2009

J Ind Microbiol Biotechnol

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Microbial modification of polyunsaturated fatty acids can often lead to special changes in their structure and in biological potential. Therefore, the aim of this study was to develop potential antifungal agents through the microbial conversion of docosahexaenoic acid (DHA). Bioconverted oil extract of docosahexaenoic acid (bDHA), obtained from the microbial conversion of docosahexaenoic acid (DHA) by Pseudomonas aeruginosa PR3, was assessed for its in vitro and in vivo antifungal potential. Mycelial growth inhibition of test plant pathogens, such as Botrytis cinerea, Colletotrichum capsici, Fusarium oxysporum, Fusarium solani, Phytophthora capsici, Rhizoctonia solani and Sclerotinia sclerotiorum, was measured in vitro. bDHA (5 microl disc(-1)) inhibited 55.30-65.90% fungal mycelium radial growth of all the tested plant pathogens. Minimum inhibitory concentrations (MICs) of bDHA against the tested plant pathogens were found in the range of 125-500 microg ml(-1). Also, bDHA had a strong detrimental effect on spore germination for all the tested plant pathogens. Further, three plant pathogenic fungi, namely C. capsici, F. oxysporum and P. capsici, were subjected to an in vivo antifungal screening. bDHA at higher concentrations revealed a promising antifungal effect in vivo as compared to the positive control oligochitosan. Furthermore, elaborative study of GC-MS analysis was conducted on bioconverted oil extract of DHA to identify the transformation products present in bDHA. The results of this study indicate that the oil extract of bDHA has potential value of industrial significance to control plant pathogenic fungi.

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Section: Discussionmentioning

confidence: 89%

Microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bDHA) used against agricultural plant pathogenic fungi

Bajpai

Kim²,

Hou

et al. 2009

J Ind Microbiol Biotechnol

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“…In addition, disease severity of verticillium wilt was assessed using a stem cut rating system at the end of the harvest stage (Dong et al . ; Huang et al . ).…”

Section: Methodsmentioning

confidence: 99%

Biocontrol of verticillium wilt and colonization of cotton plants by an endophytic bacterial isolate

Shi

Han

et al. 2012

J Appl Microbiol

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Aims To explore biocontrol potential of 39 DAEB isolates (doubly antagonistic towards both Verticillium dahliae Kleb and Fusarium oxysporum) against verticillium wilt of cotton and to elucidate colonization and category characteristics of an endophytic bacterium with significant biocontrol activity. Methods and Results Thirty‐nine antagonistic endophytic bacteria strains were tested for their ability to control verticillium wilt in cotton plants caused by a defoliating pathotype of V. dahliae 107 in cotton under controlled conditions. The biocontrol trial revealed that an endophytic bacterium, designated HA02, showed a significant biocontrol activity to V. dahliae 107. After cotton seedlings were inoculated with a gfp gene‐tagged HA02 (HA02‐gfp), HA02‐gfp populations were higher in the root than in the stem; in addition, the HA02‐gfp was distributed in the maturation zone of cotton root. Furthermore, HA02‐gfp also colonized seedlings of maize, rape and soybean after the bacteria inoculation. Phylogenetic trees based on 16S rDNA sequences combined with morphological, physiological and identification showed that the bacterium belongs to the Enterobacter genus. Conclusions Our results showed that only 1 of 39 DAEB isolates demonstrated more efficient biocontrol potential towards V. dahliae 107 in greenhouse and field trials. HA02‐gfp mainly colonized cotton in roots. In addition, we quantitatively observed HA02 colonization in other hosts. HA02 belongs to the Enterobacter genus. Significance and Impact of the Study This is the first study on biocontrol to defoliating pathotype of V. dahliae Kleb by endophytic bacteria. The HA02 showed effective biocontrol to V. dahliae 107 in greenhouse and field trials. Furthermore, we assessed the quantitative and qualitative colonization of HA02 in cotton seedlings. Our study provides basic information to further explore managing strategies to control this critical disease.

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“…A Penicillium sp. has previously been shown to have potential as a bio-control agent to reduce the cotton wilt disease caused by F. culmorum [42]. Thus the genus may have further potential as a bio-control agent for diseases caused by Fusarium spp.…”

Section: Association Of High Fungal Diversity Plentiful Organic Mattmentioning

confidence: 98%

Fusarium-suppressive effects of green manure of turnip rape

Zou

Tuulos

Mikkonen

et al. 2015

European Journal of Soil Biology

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Dry mycelium of Penicillium chrysogenum protects cotton plants against wilt diseases and increases yield under field conditions

Cited by 32 publications

References 17 publications

Microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bDHA) used against agricultural plant pathogenic fungi

Microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bDHA) used against agricultural plant pathogenic fungi

Biocontrol of verticillium wilt and colonization of cotton plants by an endophytic bacterial isolate

Fusarium-suppressive effects of green manure of turnip rape

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