Dry-powder pulmonary insufflation in the mouse for application to vaccine or drug studies

Albumin-Coated Porous Hollow Poly(Lactic-co-Glycolic Acid) Microparticles Bound with Palmityl-Acylated Exendin-4 as a Long-Acting Inhalation Delivery System for the Treatment of Diabetes

“…The pulmonary administration of various MPs was accomplished using a modification of a previously described procedure (31,32). In brief, male ICR or db/db mice were anesthetized with a single intraperitoneal (i.p.)…”

Btk Inhibitor RN983 Delivered by Dry Powder Nose-only Aerosol Inhalation Inhibits Bronchoconstriction and Pulmonary Inflammation in the Ovalbumin Allergic Mouse Model of Asthma

“…When the amount of drug is limited, direct administration methods are an option but all suffer from non-homogeneous deposition, with more drug concentrated along the central airways and less well represented in the parenchymal/alveolar regions. (29,30) Direct instillation methods including intranasal (34) or intratracheal liquid (31,32) and spray instillation (35) or dry-powder insufflation, (36,37) can be used as a screening tool to determine the approximate dose range for later inhalation studies, or to determine the ranking of efficacy/toxicity for a series of structurally similar drugs. (38) The current standard of care in asthma, inhaled b-agonist and steroid combination treatments, have a beneficial inhibition of the early asthmatic response (EAR) and the late asthmatic response (LAR), respectively.…”

Formulation of a novel fixed dose combination of salmeterol xinafoate and mometasone furoate for inhaled drug delivery

“…The laryngoscope was used to visualize the trachea, then the insufflator device was inserted into the trachea and the air pump was attached to the insufflator device. The desired volume (2 mL of air per pump for rats) of air was dispensed from the attached air pump to force the powder from the chamber of the insufflator device through the PE tube, the metal-tipped cannula, and into the trachea of the rats [37]. All powders were actuated into the lungs using 4-7 pumps.…”