Dry Reagent Chemistries

Walter, Bert

doi:10.1021/ac00255a724

Cited by 19 publications

(23 citation statements)

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“…Absorbing and drying chemical and biological reagents into a matrix such as paper was one of the first technologies to drive point-of-care devices (8,9 ). Substrates have since been expanded into membranes, films, and polymer surfaces.…”

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confidence: 99%

Microfluidic Tool Box as Technology Platform for Hand-Held Diagnostics

et al. 2005

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Background: Use of microfluidics in point-of-care testing (POCT) will require on-board fluidics, self-contained reagents, and multistep reactions, all at a low cost. Disposable microchips were studied as a potential POCT platform. Methods: Micron-sized structures and capillaries were embedded in disposable plastics with mechanisms for fluidic control, metering, specimen application, separation, and mixing of nanoliter to microliter volumes. Designs allowed dry reagents to be on separate substrates and liquid reagents to be added. Control of surface energy to ؎5 dyne/cm 2 and mechanical tolerances to <1 m were used to control flow propulsion into adsorptive, chromatographic, and capillary zones. Fluidic mechanisms were combined into working examples for urinalysis, blood glucose, and hemoglobin A 1c testing using indicators (substances that react with analyte, such as dyes, enzyme substrates, and diazonium salts), catalytic reactions, and antibodies as recognition components. Optical signal generation characterized fluid flow and allowed detection. Results: We produced chips that included capillary geometries from 10 to 200 m with geometries for stopping and starting the flow of blood, urine, or buffer; vented chambers for metering and splitting 100 nL to 30 L; specimen inlets for bubble-free specimen entry and containment; capillary manifolds for mixing; microstructure interfaces for homogeneous transfer into separation membranes; miniaturized containers for liquid storage and release; and moisture vapor barrier seals for

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confidence: 99%

Microfluidic Tool Box as Technology Platform for Hand-Held Diagnostics

et al. 2005

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“…A spectrophotometric approach to the measurement of analyses in milky appearing samples seems to be made possible by the use of reflectance measurements off of an opaque background (44,45,85). The deviation from linearity that is seen as a natural occurrence with this kind of measurement is overcome by mathematical corrective actions which linearize calibrations (86)(87)(88).…”

Section: Reflectance Spectrophotometrymentioning

confidence: 99%

A Clarifying Solution for Hypertriglyceridemic Serums

Artiss

Morgan

Zak

1997

Microchemical Journal

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“…The dry reagent test strip method is one of the one-step analytical methods that has been widely used in clinical analysis. [24][25][26] It usually consists of a small plastic bar with matrices, or reaction zones, fastened to the support; the reagents and buffers needed for the reaction are incubated on the strip. When moistening the strip with liquid samples to be investigated, the analytical reaction takes place.…”

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confidence: 99%