This study was conducted to determine the reactions of some chickpea cultivars against Rhizoctonia solani AG-4, AG-5, binucleate Rhizoctonia sp., and R. bataticola (Syn: Macrophomina phaseolina) causing root and crown rot on chickpea under in vitro and in vivo conditions. All chickpea cultivars tested against R. solani AG-4 and AG-5 isolates showed a susceptible reaction in both studies, and these agents were determined to cause pre-emergence damping-off in all plants tested. Cultivar Gökce was resistant to binucleate Rhizoctonia sp. isolate in both experiments. While all the cultivars showed a susceptible reaction to R. bataticola isolate, cultivar Aksu was evaluated as resistant in the trial. According to our knowledge, this is the first study on the determination of the reactions of chickpea cultivars against Rhizoctonia spp. in Turkey.