Dry to wet weight biomass conversion constant for Tetrahymena elliotti (Ciliophora, Protozoa)

Gates, Michael A.; Rogerson, Andrew; Berger, Jacques

doi:10.1007/bf00384479

Cited by 51 publications

(30 citation statements)

References 18 publications

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“…Experimentally determined specific gravities and dry matter content for bacteria and fungal hyphae range from 1.04 to 1.13 pg prne3 and 12 to 33% (Bakken and Olsen 1983;Bratbak and Dundas 1984). These values are consistent with the range of dry weight : volume ratios of 0.16-0.28 pg pm-3 measured for the ciliates Colpidium campylum and Tetrahymena elliotti (Laybourn and Finlay 1976;Rogerson 198 1;Gates et al 1982). Determining an appropriate C : vol ratio based on theoretical considerations is further complicated for many practical situations where cell volumes are determined microscopically from linear cell dimensions of preserved material.…”

Section: Notessupporting

confidence: 85%

An experimentally determined carbon : volume ratio for marine “oligotrichous” ciliates from estuarine and coastal waters

Putt

Stoecker

1989

Limnology & Oceanography

975

517

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The biomass of marine “oligotri chous” ciliates has often been estimated by measuring the cell volume of preserved samples and converting to units of carbon based on theoretical carbon: volume (C: vol) ratios of 0.07–0.11 pg µm−3. Using laboratory cultures of several strains of Laboea strobila, Strombidium spp., and Strobilidium spiralis, we experimentally derived a C: vol conversion factor of 0.19 pg µm−3 for cells preserved with 2% vol : vol Lugol’s iodine. Cell volume estimates of Lugol’s‐preserved cells averaged 76% of cell volume estimates of Formalin‐preserved cells. Hence a C : vol ratio of 0.14 pg µm−3 applies to Formalin‐preserved cells. Our study indicates that the biomass of oligotrichous ciliates in marine systems has been significantly underestimated by the use of inappropriate C: vol ratios.

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Section: Notessupporting

confidence: 85%

An experimentally determined carbon : volume ratio for marine “oligotrichous” ciliates from estuarine and coastal waters

Putt

Stoecker

1989

Limnology & Oceanography

975

517

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“…14 °C) fuel a faster degradation of transient high-molecular weight free DNA compared to 'normal' cold deep-sea environments. Live cells on the other hand would not be able to penetrate even the uppermost layers of the interface as their typical specific density is 1.025 (Beaver and Crisman 1982), or less (Gates et al 1982), and even hard-bodied benthic metazoa are lighter than brine (Epstein 1995). It therefore stands to reason that our samples from at least the denser brines were largely free of allochtonous cells and their DNA, and that at least the vast majority of detected 18S rRNA gene sequences represent the indigenous protistan communities.…”

Section: Resultsmentioning

confidence: 99%

Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea

et al. 2008

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Non-standard abbreviations:DHAB -deep hypersaline anoxic basin UMA -uncultured marine alveolate clade 2 AbstractEnvironmental factors restrict the distribution of microbial eukaryotes but the exact boundaries for eukaryotic life are not known. Here we examine protistan communities at the extremes of salinity and osmotic pressure, and report rich assemblages inhabiting Bannock and Discovery, two deep-sea superhaline anoxic basins in the Mediterranean. Using a rRNA-based approach, we detected 1538 protistan rRNA gene sequences from water samples with total salinity ranging from 39 g/kg to 280 g/Kg, and obtained evidence that this DNA was endogenous to the extreme habitats sampled. Statistical analyses indicate that the discovered phylotypes represent only a fraction of species actually inhabiting both the brine and the brine-seawater interface, with as much as 82% of the actual richness missed by our survey. Jaccard indices (e.g., for a comparison of community membership) suggest that the brine/interface protistan communities are unique to Bannock and Discovery basins, and share little (0.8-2.8%) in species composition with overlying waters with typical marine salinity and oxygen tension. The protistan communities from the basins' brine and brine/seawater interface appear to be particularly enriched with dinoflagellates, ciliates and other alveolates, as well as fungi, and are conspicuously poor in stramenopiles. The uniqueness and diversity of brine and brine-interface protistan communities make them promising targets for protistan discovery.

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“…At least 50 individuals were counted and sized (maximum length and width) to the nearest 2.4 ,um in each sample. Cell volumes of the ciliates were estimated by assuming that all cells approximated prolate spheroids, and volumes were then converted to dry weight assuming 0.279 pg dry wt ,um-3 (Gates et al 1982).…”

Section: Methodsmentioning

confidence: 99%

An empirical analysis of zooplankton community size structure across lake trophic gradients1

Pace

1986

Limnology & Oceanography

236

135

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The hypothesis was tested that zooplankton community size structure shifts toward an increased relative biomass of microzooplankton with increased lake trophy at 12 sites in Quebec, The seasonal mean abundance and biomass of ciliates, rotifers, nauplii, cladocerans, and cyclopoid copepods were significantly (P < 0.1) related to lake trophy, but Calanoid copepod abundance and biomass varied independently of lake trophy. Regressions of microzooplankton and macrozooplankton biomass with total phosphorus (TP) were highly significant (P < 0.000 l), and TP explained a large proportion of the total variation (microzooplankton: Y* = 0.72; macrozooplankton: r2 = 0.86). The regression models for microzooplankton and macrozooplankton were not significantly different, refuting the hypothesis that relative biomass changes with lake trophy. Further analysis with a community structure index (the slope of the log weight-log abundance relationship) and mean lengths of various taxa indicated that zooplankton community size structure was not correlated with either TP or chlorophyll. On average, about 40% of the total zooplankton biomass is accounted for by microzooplankton in the Quebec lakes. The inverse relationship between body size and specific flux rates suggests that microzooplankton account for the major portion of zooplankton community rates.

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Dry to wet weight biomass conversion constant for Tetrahymena elliotti (Ciliophora, Protozoa)

Cited by 51 publications

References 18 publications

An experimentally determined carbon : volume ratio for marine “oligotrichous” ciliates from estuarine and coastal waters

An experimentally determined carbon : volume ratio for marine “oligotrichous” ciliates from estuarine and coastal waters

Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea

An empirical analysis of zooplankton community size structure across lake trophic gradients1

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