Dry Weight and Nitrogen Content of "Schistosoma mansoni" from Experimental Infections

Lincicome, David Richard; Eni, E. U.; Stirewált, M. A.

doi:10.2307/1292974

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…to a maximum average dry weight of 017 mg at 100 days p.i. Detailed comparison of these average individual dry weights with those given by other workers using different methods for drying the worms (for example, drying at 95-105 °C for 18-24 h (Radke, Schneider & Houghtaling, 1957), drying in acetone (Lincicome, Eni & Stirewalt, 1963), freeze drying (Lennox & Schiller, 1972) is inappropriate because of the different techniques used, although close similarities, in particular with the results of Lennox & Schiller (1972), do exist.…”

Section: Discussionmentioning

confidence: 85%

Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms

Shaw¹,

Erasmus²

1983

Parasitology

View full text Add to dashboard Cite

Changes during development in the elemental contents (Ca, P, K, Mg and S) of male and female Schistosoma mansoni from both mixed and single sex infections have been measured for the first time. In mixed infections, there is a significant difference between the calcium content of male and female worms; female worms having between 2 and 3 times more calcium. In mature female worms from mixed infections, after an initial rise in the calcium content corresponding to the time when vitelline development occurs, the amount of calcium ranged from 0.36 to 0.65 mg/g dry wt, whereas the amounts in similarly aged male worms ranged between 0.19 and 0.32 mg/g dry wt. While the type of infection does not affect the calcium content of male worms, a significant difference in the calcium levels in female worms from mixed and single sex infections was found. The amount of calcium in females from single sex infections ranged from 0.21 to 0.39 mg/g dry wt and this is considerably less than the levels recorded in females from mixed infections. This difference in calcium content between mixed and single sex infections can be related to the differences in degree of development of the vitelline gland in the two types of female worm. The amounts of phosphorus, potassium, magnesium and sulphur in both male and female worms from both single and mixed infections are similar, although females from mixed infections contained slightly more phosphorus than either females from single sex infections or either type of male worm.

show abstract

Section: Discussionmentioning

confidence: 85%

Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms

Shaw¹,

Erasmus²

1983

Parasitology

View full text Add to dashboard Cite

show abstract

Distribution and excretion of C14-labeled ABBOTT-16612 in experimental animals

Miller

Alter

Cardinal

et al. 1966

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Protein, Glycogen, and Water Content in Schistosomes

Cornford¹,

Huot²,

Diep³

et al. 1982

The Journal of Parasitology

View full text Add to dashboard Cite

The volume of distribution (= VD) of water was measured in Schistosoma mansoni, S. haematobium, S. japonicum, and Hymenolepis diminuta. In the rat tapeworm, H. diminuta, the volume of distribution of 3H-water was positively correlated with wet weight (r = 0.87, P less than 0.001) and this same phenomenon also was demonstrated in S. mansoni (r = 0.90, P less than 0.001). The 5-sec VDwater was constant over a range of glucose (0.01-50 mM) and phenylalanine (0.01-20 mM) concentrations in both male and female schistosomes. The VD of antipyrine, which permeates by virtue of its lipophilic properties, also was shown to correlate with protein content in S. mansoni. Protein content determined in single, isolated schistosomes was correlated with the volume of distribution of water in S. haematobium, S. japonicum, and S. mansoni males and females. Age-related variations in the protein content (of S. mansoni) and volume of distribution of water (in both S. japonicum and S. mansoni) were also defined, and the use of tritiated water content as an indicator of mass in small tissue samples was thus established. Female blood flukes recovered from mice infected for more than 90 days appeared to be characterized by a slight reduction in size with age. Schistosoma mansoni reared in the golden hamster may be slightly smaller than schistosomes of the same strain raised in outbred mice. These results provide a baseline that should be useful for future physiological and immunological studies.

show abstract

Dry Weight and Nitrogen Content of "Schistosoma mansoni" from Experimental Infections

Cited by 3 publications

References 0 publications

Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms

Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms

Distribution and excretion of C14-labeled ABBOTT-16612 in experimental animals

Protein, Glycogen, and Water Content in Schistosomes

Contact Info

Product

Resources

About