Brunensis, 2013, LXI, No. 6, pp. 1769-1777 Water sorption tests of Roselle (Hibiscus sabdariff a L.) carried out under laboratory conditions are presented together with mathematical analyses of the moisture sorption isotherms (MSI's). Moisture equilibrium data for adsorption and desorption of water from Roselle powder were investigated at near ambient air temperatures in the range of 5 and 35 °C and water activity (Aw) ranging from 0.11 to 0.97. The manometric method has been used for water sorption tests. Models for MSI's are exponential equations. Coeffi cients of determination are 0.998 and 0.996 (for adsorption and desorption at 5 °C, respectively), 0.998 and 0.999 (for adsorption and desorption at 20 °C, respectively), and 0.998 and 0.999 (for adsorption and desorption at 35 °C, respectively). The equilibrium moisture content (EMC) of Roselle samples increased with an increase of Aw at a constant temperature both for adsorption and desorption. Adsorption curve equates to desorption curve at higher temperatures of tests carried out. Critical values of EMC of samples tested corresponding to the Aw equal to 0.6 were between 13.401% moisture content wet basis (MC w.b.) and 15.934% MC (w.b.) for moisture adsorption and desorption, respectively. These values are useful for storing conditions optimisation from point of view microorganisms grow and structural changes analyses. Crystal structure changes were observed during adsorption and desorption in the microscope, too. It was found out glass transition in dependence on the water content of samples tested.water activity, equilibrium moisture content, hysteresis, prediction, grow of microorganisms, glass transition Roselle (Hibiscus sabdariff a L.) belongs to the family Malvaceae. It originated in India and it is widely distributed in tropical and subtropical regions as a potential new food crop of considerable economic potential. Roselle is a short-day annual erect shrub and can grow to a height of 1-3 m, depending on the species. Its calyces have been suggested for the production of so drinks, Roselle tea, jam, juices, and natural food colourants (Chen et al., 2005;Chang et al., 2012). The extracts of calyces and leaves of Roselle show a signifi cant antihyperlipidemic activity (Ochani and D'Mello, 2009).Roselle calyx (Fig. 1) is usually harvested at high MC (w.b.), 85%. Therefore, drying is an important postharvest treatment prior to reduce the MC and to increase the shelf life. Moreover, the main purpose of drying the products is to minimize packaging requirements, to prolong the shelf life and to reduce shipping weights (Vengaiah and Pandey, 2007). Tab. I shows the chemical composition of Roselle calyces (Babalola et al., 2001).