Drying Enhances Signal Intensities for Global GC–MS Metabolomics

Liebeke, Manuel; Puskas, Erik

doi:10.3390/metabo9040068

Cited by 15 publications

(11 citation statements)

References 21 publications

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“…These steps break apart the salt crystals and release water into the toluene to enhance salt drying and metabolite extraction. Finally, following a recently described method for improving GC-MS metabolite detection regardless of sample type (33), we included an additional step between the methoximation and trimethylsilylation derivatization reactions and evaporated the first derivatization reagent under N 2 gas (see Fig. 1B for total signal improvements of each step for the metabolite mixture).…”

Section: Resultsmentioning

confidence: 99%

Marine Metabolomics: a Method for Nontargeted Measurement of Metabolites in Seawater by Gas Chromatography–Mass Spectrometry

et al. 2019

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Nontargeted approaches using metabolomics to analyze metabolites that occur in the oceans is less developed than those for terrestrial and limnic ecosystems. One of the challenges in marine metabolomics is that salt limits metabolite analysis in seawater to methods requiring salt removal. Building on previous sample preparation methods for metabolomics, we developed SeaMet, which overcomes the limitations of salt on metabolite detection. Considering that the oceans contain the largest dissolved organic matter pool on Earth, describing the marine metabolome using nontargeted approaches is critical for understanding the drivers behind element cycles, biotic interactions, ecosystem function, and atmospheric CO2 storage. Our method complements both targeted marine metabolomic investigations as well as other “omics” (e.g., genomics, transcriptomics, and proteomics) approaches by providing an avenue for studying the chemical interaction between marine microbes and their habitats.

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Section: Resultsmentioning

confidence: 99%

Marine Metabolomics: a Method for Nontargeted Measurement of Metabolites in Seawater by Gas Chromatography–Mass Spectrometry

et al. 2019

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“…Metabolite derivatization was performed by adding 80 µL of methoxyamine hydrochloride dissolved in pyridine (20 mg mL -1 ) to the dried pellet and incubating for 90 min at 37 °C using a thermal rotating incubator under constant rotation at 1350 rpm. Following recent advancements in signal improvement 58 , the pyridine was removed from the sample at room temperature under a gentle flow of N 2 gas (approximately 1 hour). Following the addition of 100 µL of N,O-B is(trimethylsilyl)trifluoroacetamide, each extract was vortexed, and incubated for another 30 min at 37 °C using a thermal rotating incubator under constant rotation at 1350 rpm.…”

Section: Methodsmentioning

confidence: 99%

Sugars dominate the seagrass rhizosphere

Sogin

Michellod

Gruber‐Vodicka

et al. 2019

Preprint

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14Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth1. Despite 15 covering less than 0.1 % of coastal regions, they have the capacity to bury up to 10 % of marine 16 organic matter1 and can bury the same amount of carbon 35 times faster than tropical 17 rainforests2. On land, the soil's ability to sequestrate carbon is intimately linked to microbial 18 metabolism3. Despite the growing attention to the link between plant production, microbial 19 communities, and the carbon cycle in terrestrial ecosystems4-6, these processes remain enigmatic 20 in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, 21 into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do 22

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“…This feature makes the identification of unknown compounds in GC-MS more straightforward than the LC-MS approach. Nonetheless, GC-MS can only be used to analyze volatile compounds; thus, a derivatization step is often necessary during the analysis of key metabolites, such as amino acids, sugars, and fatty acids [ 59 , 60 ].…”

Section: Application Of Metabolomics In the Study Of Biological Responses To Environmental Toxicantsmentioning

confidence: 99%

“…In addition to the HILIC approach, GC-MS analysis with derivatization has long been the preferred method for the analysis of polar primary metabolites. Amino acids and carbohydrates have also been analyzed using various silylation agents, when methylation agents are often preferred for the analysis of fatty acids [ 59 , 81 , 89 ]. Furthermore, metabolites that can be analyzed via GC-MS are often involved in key metabolic pathways related to energy production, such as glycolysis, TCA cycle, and free fatty acid metabolism, in model organisms [ 90 , 91 ].…”

Section: Metabolomics Workflowmentioning

confidence: 99%

Metabolomic Studies for the Evaluation of Toxicity Induced by Environmental Toxicants on Model Organisms

Kim

Kang

2021

Metabolites

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Environmental pollution causes significant toxicity to ecosystems. Thus, acquiring a deeper understanding of the concentration of environmental pollutants in ecosystems and, clarifying their potential toxicities is of great significance. Environmental metabolomics is a powerful technique in investigating the effects of pollutants on living organisms in the environment. In this review, we cover the different aspects of the environmental metabolomics approach, which allows the acquisition of reliable data. A step-by-step procedure from sample preparation to data interpretation is also discussed. Additionally, other factors, including model organisms and various types of emerging environmental toxicants are discussed. Moreover, we cover the considerations for successful environmental metabolomics as well as the identification of toxic effects based on data interpretation in combination with phenotype assays. Finally, the effects induced by various types of environmental toxicants in model organisms based on the application of environmental metabolomics are also discussed.

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Drying Enhances Signal Intensities for Global GC–MS Metabolomics

Cited by 15 publications

References 21 publications

Marine Metabolomics: a Method for Nontargeted Measurement of Metabolites in Seawater by Gas Chromatography–Mass Spectrometry

Marine Metabolomics: a Method for Nontargeted Measurement of Metabolites in Seawater by Gas Chromatography–Mass Spectrometry

Sugars dominate the seagrass rhizosphere

Metabolomic Studies for the Evaluation of Toxicity Induced by Environmental Toxicants on Model Organisms

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