Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway

Zhang, Ying; Luo, Meng; Zu, Yuangang; Fu, Yujie; Gu, Cheng‐Bo; Wang, Wei; Yao, Liping; Efferth, Thomas

doi:10.1016/j.cbi.2012.06.007

Cited by 60 publications

(42 citation statements)

References 43 publications

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“…First, free radicals may attack membrane phospholipids, causing mitochondrial membrane depolarization and the release of mitochondrial factors. This intrinsic apoptosis pathway is subsequently involved in the activation of caspases and DNA fragmentation (9).…”

Section: Introductionmentioning

confidence: 99%

“…Several novel anticancer agents extracted from plants, such as dryofragin (9), diallyl trisulfide (8) and levamisole (10) can inhibit various types of cancer cells by inducing apoptosis via the generation of ROS and the disruption of mitochondrial transmembrane potential. Centratherum anthelminticum (L.) seeds induce apoptosis in breast cancer MDA-MB-231 cells, which is associated with ROS generation and downregulation of the mitochondrial proteins Bcl2 and Bcl-xl (11).…”

Section: Introductionmentioning

confidence: 99%

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Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Jin

Park

Kim

et al. 2013

International Journal of Oncology

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Abstract. Halofuginone (HF) is extracted from Dichroa febrifuga, a plant used in traditional medicine. We report that the HF extract inhibits the growth of breast cancer cells and induces the generation of reactive oxygen species (ROS) and apoptosis, an important feature of potential anticancer agents. In addition, HF significantly reduces the migration and invasion of MCF-7 and MDA-MB-231 human breast cancer cells after 12-O-tetraecanoylphorbol-13-acetate (TPA) stimulation. As matrix metalloproteinase-9 plays a critical role in tumor metastasis, we analyzed its expression with the HF extract treatment. Western blot analysis and gelatin zymography showed that HF suppresses MMP-9 expression and activity concentrationdependently. HF also decreases the nuclear protein levels of nuclear factor kappa B (NF-κB) and c-fos (AP-1), critical transcription factors regulating MMP-9 expression through binding the MMP-9 promoter region. Luciferase assays showed that HF decreases TPA-induced MMP-9 promoter binding activities of NF-κB and AP-1. Taken together, these are the first results indicating that halofuginone may represent a promising new agent for breast cancer chemotherapy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Jin

Park

Kim

et al. 2013

International Journal of Oncology

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“…Dryofragin induces human breast cancer MCF-7 cells apoptosis through ROS-mediated mitochondria-dependent pathway. Dryofragin inhibited Bcl-2 and induced Bax expression, which led to activation of caspases-3 in the cytosol, and further cleavage of poly ADP-ribose polymerase (PARP) in the nucleus, and then induced cell apoptosis (Zhang et al, 2012). The phenolic hydroxyls exhibited highly pro-apoptotic activity DOI:http://dx.doi.org/10.7314/APJCP.2016.17.4.1823 through the mitochondria-mediated pathway.…”

Section: Discussionmentioning

confidence: 99%

Dryocrassin ABBA Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells Through a Caspase-Dependent Mitochondrial Pathway

Jin¹,

Wang²,

Huang³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Background: Biological and pharmacological activities of dryocrassin ABBA, a phloroglucinol derivative extracted from Dryopteris crassirhizoma, have attracted attention. In this study, the apoptotic effect of dryocrassin ABBA on human hepatocellular carcinoma HepG2 cells was investigated. Materials and Methods: We tested the effects of dryocrassin ABBA on HepG2 in vitro by MTT, flow cytometry, real-time PCR, and Western blotting. KM male mice were used to detect the effect of dryocrassin ABBA on H22 cells in vivo. Results: Dryocrassin ABBA inhibited the growth of HepG2 cells in a concentration-dependent manner. After treatment with 25, 50, and 75 μg/mL dryocrassin ABBA, the cell viability was 68%, 60% and 49%, respectively. Dryocrassin ABBA was able to induce apoptosis, measured by propidium iodide (PI)/annexin V-FITC double staining. The results of real-time PCR and Western ting showed that dryocrassin ABBA up-regulated p53 and Bax expression and inhibited Bcl-2 expression which led to an activation of caspase-3 and caspase-7 in the cytosol, and then induction of cell apoptosis. In vivo experiments also showed that dryocrassin ABBA treatment significantly suppressed tumor growth, without major side effects. Conclusions: Overall, these findings provide evidence that dryocrassin ABBA may induce apoptosis in human hepatocellular carcinoma cells through a caspase-mediated mitochondrial pathway.

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“…Zhang et al (2012), showed that dryofragin inhibited the growth of MCF-7 cells inducing apoptosis through mitochondrion-dependent pathway with the involvement of reactive oxygen species (ROS) production. Another phloroglucinol derivative isolated from E. cava, dioxinodehydroeckol, has a potential inhibitory effect on proliferation of MCF-7 and MDA-MB231 cells.…”

Section: Phloroglucinolmentioning

confidence: 99%

Bioactive compounds from brown seaweeds: Phloroglucinol, fucoxanthin and fucoidan as promising therapeutic agents against breast cancer

Pádua

Rocha

Gargiulo

et al. 2015

Phytochemistry Letters

169

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Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway

Cited by 60 publications

References 43 publications

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Dryocrassin ABBA Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells Through a Caspase-Dependent Mitochondrial Pathway

Bioactive compounds from brown seaweeds: Phloroglucinol, fucoxanthin and fucoidan as promising therapeutic agents against breast cancer

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