DsRNA degradation in the pea aphid (Acyrthosiphon pisum) associated with lack of response in RNAi feeding and injection assay

Christiaens, Olivier; Swevers, Luc; Smagghe, Guy

doi:10.1016/j.peptides.2013.12.014

Cited by 234 publications

(223 citation statements)

References 47 publications

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“…A variety of factors including nucleases in the midgut (Liu et al, 2013), salivary secretions (Allen and Walker, 2012;Christiaens et al, 2014) and haemolymph (Garbutt et al, 2013;Christiaens et al, 2014) have shown to degrade dsRNA in different insect orders and may be important in determining specificity. The results from the diet stability experiment suggest that dsRNA enzymatic barriers are most likely occurring after ingestion since no dsRNA degradation was observed from salivary secretions released in both larval and adult diet (Fig.…”

Section: Discussionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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“…In the digestive juice of larvae of the silkworm, Bombyx mori, an alkaline nuclease that could digest dsRNA has been isolated (Arimatsu et al, 2007). Later, high nuclease activity also has been demonstrated in the gut juice of locusts (Luo et al, 2013;Wynant et al, 2014b), as well as in salivary secretions of the pea aphid, Acyrthosiphon pisum (Christiaens et al, 2014), and the tarnished plant bug, Lygus lineolarus (Allen and Walker, 2012), to name a few. It is therefore very plausible that dsRNA degradation in the alimentary tract of insects contributes to a lowered RNAi efficiency upon feeding on dsRNA.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Spit

Philips

Wynant

et al. 2017

Insect Biochemistry and Molecular Biology

137

141

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Abstract:The responsiveness towards orally delivered dsRNA and the potency of a subsequent environmental RNA interference (RNAi) response strongly differs between different insect species. While some species are very sensitive to dsRNA delivery through the diet, others are not. The underlying reasons for this may vary, but degradation of dsRNA by nucleases in the gut lumen is believed to play a crucial role. The Colorado potato beetle, Leptinotarsa decemlineata, is a voracious defoliator of potato crops worldwide, and is currently under investigation for novel control methods based on dsRNA treatments. Here we describe the identification and characterization of two nuclease genes exclusively expressed in the gut of this pest species. Removal of nuclease activity in adults increased the sensitivity towards dsRNA and resulted in improved protection of potato plants. A similar strategy in the desert locust, Schistocerca gregaria, for which we show a far more potent nuclease activity in the gut juice, did however not lead to an improvement of the RNAi response.Possible reasons for this are discussed. Taken together, the present data confirm a negative effect of nucleases in the gut on the environmental RNAi response, and further suggest that interfering with this activity is a strategy worth pursuing for improving RNAi efficacy in insect pest control applications.

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“…For example, the pea aphid possesses potent nuclease activity in its hemolymph. 51 In insects studied to date, differences in persistence of dsRNA injected into the hemolymph has correlated well with RNAi sensitivity. 54,129,130 When discussing systemic RNAi in invertebrates, it is important to point out that C. elegans and some other invertebrates also possesses a system that amplifies a primary siRNA to give rise to a secondary siRNA.…”

Section: Are Srnas Disseminated Systemically?mentioning

confidence: 99%

“…49 However, dsRNAs are susceptible to breakdown like all macromolecules and nuclease activity has been characterized in multiple regions of the digestive tract of various insects. For example nuclease activity against dsRNA has been found in the saliva of the tarnished plant bug 50 and pea aphid 51 as well as the midgut of the silk worm, 52 the desert locust, 53 and others. 54 It is important to note that the digestive tract tissue architecture and environment are quite divergent within invertebrates, 55,56 leading to differences in potential for sRNA degradation.…”

Section: Introductionmentioning

confidence: 99%

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

Chan

Snow

2016

RNA Biology

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The putative transfer and gene regulatory activities of diet-derived small RNAs (sRNAs) in ingesting animals are still debated. The existence of natural uptake of diet-derived sRNA by invertebrate species could have significant implication for our understanding of ecological relationships and could synergize with efforts to use RNA interference (RNAi) technology in agriculture. Here, we synthesize information gathered from studies in invertebrates using natural or artificial dietary delivery of sRNA and from studies of sRNA in vertebrate animals and plants to review our current understanding of uptake and impact of natural dietderived sRNA on invertebrates. Our understanding has been influenced and sometimes confounded by the diversity of invertebrates and ingested plants studied, our limited insights into how gene expression may be modulated by dietary sRNAs at the mechanistic level, and the paucity of studies focusing directly on natural uptake of sRNA. As such, we suggest 2 strategies to investigate this phenomenon more comprehensively and thus facilitate the realization of its potentially broad impact on ecology and agriculture in the future.

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DsRNA degradation in the pea aphid (Acyrthosiphon pisum) associated with lack of response in RNAi feeding and injection assay

Cited by 234 publications

References 47 publications

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

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