Dual-action silver functionalized nanostructured titanium against drug resistant bacterial and fungal species

Huang, Louisa Z.Y.; Elbourne, Aaron; Shaw, Z. L.; Cheeseman, Samuel; Goff, Abigail; Orrell-Trigg, Rebecca; Chapman, James; Murdoch, Billy J.; Crawford, Russell J.; Friedmann, Donia; Bryant, Saffron J.; Truong, Vi Khanh; Caruso, Rachel A.

doi:10.1016/j.jcis.2022.08.052

Cited by 21 publications

(13 citation statements)

References 79 publications

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“…Advances are also being made in C. auris eradication from patients’ rooms. In addition to silver nanoparticles, silver functionalized nanostructured titanium has recently been recognized as a promising antifungal agent against growth and biofilm formation of C. auris on medical and environmental surfaces [ 121 ]. Far ultraviolet subtype C (222 nm) (Far-UVC) has recently been shown to destroy airborne pathogens nearly instantly in room-sized chambers [ 122 ].…”

Section: Future Perspectivesmentioning

confidence: 99%

Strategies to Prevent Transmission of Candida auris in Healthcare Settings

Ahmad

Asadzadeh

2023

Curr Fungal Infect Rep

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Purpose of Review Candida auris , a recently recognized yeast pathogen, has become a major public health threat due to the problems associated with its accurate identification, intrinsic and acquired resistance to antifungal drugs, and its potential to easily contaminate the environment causing clonal outbreaks in healthcare facilities. These outbreaks are associated with high mortality rates particularly among older patients with multiple comorbidities under intensive care settings. The purpose of this review is to highlight strategies that are being adapted to prevent transmission of C. auris in healthcare settings. Recent Findings Colonized patients shed C. auris into their environment which contaminates surrounding equipment. It resists elimination even by robust decontamination procedures and is easily transmitted to new patients during close contact resulting in outbreaks. Efforts are being made to rapidly identify C. auris -infected/ C. auris -colonized patients, to determine its susceptibility to antifungals, and to perform effective cleaning and decontamination of the environment and isolation of colonized patients to prevent further transmission. Summary Rapid and accurate identification of hospitalized patients infected/colonized with C. auris , rapid detection of its susceptibility patterns, and appropriate use of infection control measures can help to contain the spread of this highly pathogenic yeast in healthcare settings and prevent/control outbreaks.

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Section: Future Perspectivesmentioning

confidence: 99%

Strategies to Prevent Transmission of Candida auris in Healthcare Settings

Ahmad

Asadzadeh

2023

Curr Fungal Infect Rep

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“…109 However, it is routinely reported that nanostructured surfaces made from a variety of materials can induce high rates of bacterial and fungal cell lysis in the absence of external factors. 10,17,21,22,24,25,110 Bandara et al 111 suggested that it is the extracellular polymeric substance secreted by bacterial cells that makes contact with the substrate, rather than the membrane, and that it plays a major role in the initial adhesion stages of bacteria. Linklater et al 112 provided contradictory evidence to this by using extracellular polymeric substance-deficient mutant bacterial strains, showing that the mechanoresponsive mechanism was still observed.…”

Section: Acs Applied Bio Materialsmentioning

confidence: 99%

“…One promising technology is surface nanostructuring, whereby a nanoarchitecture is incorporated onto the surface of a material, rendering it unsuitable for microbial colonization. − Nanostructures can either limit the attachment of microbes and therefore the initial formation of a biofilm ,,− or induce cell lysis upon surface contact via damage of the cell membrane leading to cellular death. ,− The latter mechanism is referred to as a mechano-microbiocidal response, whereby bacterial and fungal cells self-rupture as they adsorb onto the nanostructured surface. Here, the membranes of a microbe must stretch beyond their elastic limit under the influence of natural adhesion forces.…”

Section: Introductionmentioning

confidence: 99%

“…Many different nanoarchitectures have been investigated to impart high antimicrobial activity on various surfaces. Such structures include pillars, , wires, , and blades . Importantly, this mechanism is independent of surface chemistry, limiting the ability of microbes to develop a protective response. ,,,− Although these surfaces show inherent antimicrobial activity, one aspect remains hotly debated: what are the underlying forces and biophysical properties that govern mechanoresponsive antimicrobial action?…”

Section: Introductionmentioning

confidence: 99%

“…Many different nanoarchitectures have been investigated to impart high antimicrobial activity on various surfaces. Such structures include pillars, 21,27 wires, 17,19 and blades. 17 Importantly, this mechanism is independent of surface chemistry, limiting the ability of microbes to develop a protective response.…”

Section: ■ Introductionmentioning

confidence: 99%

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Cell Adhesion, Elasticity, and Rupture Forces Guide Microbial Cell Death on Nanostructured Antimicrobial Titanium Surfaces

Huang,

Shaw,

Penman

et al. 2023

ACS Appl. Bio Mater.

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Naturally occurring and synthetic nanostructured surfaces have been widely reported to resist microbial colonization. The majority of these studies have shown that both bacterial and fungal cells are killed upon contact and subsequent surface adhesion to such surfaces. This occurs because the presence of high-aspect-ratio structures can initiate a self-driven mechanical rupture of microbial cells during the surface adsorption process. While this technology has received a large amount of scientific and medical interest, one important question still remains: what factors drive microbial death on the surface? In this work, the interplay between microbial-surface adhesion, cell elasticity, cell membrane rupture forces, and cell lysis at the microbial-nanostructure biointerface during adsorptive processes was assessed using a combination of live confocal laser scanning microscopy, scanning electron microscopy, in situ amplitude atomic force microscopy, and single-cell force spectroscopy. Specifically, the adsorptive behavior and nanomechanical properties of live Gram-negative (Pseudomonas aeruginosa) and Gram-positive (methicillin-resistant Staphylococcus aureus) bacterial cells, as well as the fungal species Candida albicans and Cryptococcus neoformans, were assessed on unmodified and nanostructured titanium surfaces. Unmodified titanium and titanium surfaces with nanostructures were used as model substrates for investigation. For all microbial species, cell elasticity, rupture force, maximum cell-surface adhesion force, the work of adhesion, and the cell-surface tether behavior were compared to the relative cell death observed for each surface examined. For cells with a lower elastic modulus, lower force to rupture through the cell, and higher work of adhesion, the surfaces had a higher antimicrobial activity, supporting the proposed biocidal mode of action for nanostructured surfaces. This study provides direct quantification of the differences observed in the efficacy of nanostructured antimicrobial surface as a function of microbial species indicating that a universal, antimicrobial surface architecture may be hard to achieve.

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