Dual amplified electrochemical sensing coupling of ternary hybridization-based exosomal microRNA recognition and perchlorate-assisted electrocatalytic cycle

Wang, Liangliang; Wu, Xiufeng; Chen, Guanyu; Chen, Yawen; Xu, Lilan; Wang, Jianmin; Chen, Jinghua

doi:10.1016/j.bios.2023.115783

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…Exosomes are nanoscale extracellular vesicles released from multiple cells and contain proteins, lipids, DNA, and RNA. − An increasing number of studies indicate that exosomal biomolecules are highly disease-related. In particular, exosomal miRNAs promote the development and progression of cancer and are considered reliable biomarkers for noninvasive cancer diagnosis. − However, it is difficult to achieve accurate classification of cancer based on single miRNAs in clinical diagnosis because the expression levels of identical miRNA markers on different cancer-related exosomes may have only slight differences. − Therefore, the analysis of multiple miRNAs on a single exosome is urgently needed for precision medicine.…”

Section: Introductionmentioning

confidence: 99%

DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ

Liu,

Zhao,

Chen

et al. 2024

Anal. Chem.

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Exosomal miRNAs are considered promising biomarkers for cancer diagnosis, but their accuracy is severely compromised by the low content of miRNAs and the large amount of exosomal miRNAs released from normal cells. Here, we presented a dual-specific miRNA’s logical recognition triggered by an entropy-driven catalysis (EDC)-enhanced system in exosomes for accurate detection of liver cancer-cell-derived exosomal miR-21 and miR-122. Taking advantage of the accurate analytical performance of the logic device, the excellent membrane penetration of gold nanoparticles, and the outstanding amplification ability of the EDC reaction, this method exhibits high sensitivity and selectivity for the detection of tumor-derived exosomal miRNAs in situ. Moreover, due to its excellent performance, this logic device can effectively distinguish liver cancer patients from healthy donors by determining the amount of cancer-cell-derived exosomal miRNAs. Overall, this strategy has great potential for analyzing various types of exosomes and provides a viable tool to improve the accuracy of cancer diagnosis.

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Section: Introductionmentioning

confidence: 99%

DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ

Liu,

Zhao,

Chen

et al. 2024

Anal. Chem.

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A disposable electrochemical magnetic immunosensor for the rapid and sensitive detection of 5-formylcytosine and 5-carboxylcytosine in DNA

Zhao,

Guo,

Chen

et al. 2024

Biosensors and Bioelectronics

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Electrochemical Biosensor Based on Dual-Ligand Functionalized Lanthanide-Encapsulated Polyoxometalate Conductive Polymer Film for Detecting Broad-Spectrum Tumor Marker MicroRNA-155

Meng,

Jia,

Tang

et al. 2024

Inorg. Chem.

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In this work, a dual-ligand functionalized lanthan i d e -e n c a p s u l a t e d s e l e n o t u n g s t a t e [ H 2 N -(CH 3 ) 2 ] 16 Na 2 H 10 [Ho 6 (H 2 O) 10 (HPACA) 4 W 10 O 28 (Ac) 2 ]-[SeW 9 O 33 ] 6 • 60H 2 O (1, HPACA = 2-pyrazinecarboxylic acid, HAc = acetic acid) was successfully acquired by simultaneously incorporating rigid HPACA and flexible Ac − ligands to one r e a c t i o n s y s t e m . I n t e r e s t i n g l y , t h e p o l y a n i o n [Ho 6units interconnected through an organic−inorganic hybrid dual-ligand bimetallic [Ho 6 (H 2 O) 10 (HPACA) 4 W 10 O 28 (Ac) 2 ] 20+ cluster. Moreover, the 1@PNMPy film (PNMPy = poly(N-methylpyrrole)) was successfully prepared through an electrochemical polymerization strategy. The doping of 1 significantly narrows the bandgap in the 1@PNMPy film, which enables the 1@PNMPy film to exhibit remarkable conductivity and rapid electron transfer capability. Then, the 1@PNMPy film-modified glassy carbon electrode was used to construct a 1@PNMPy-based electrochemical biosensor (ECBS), which achieves sensitive electrochemical detection (a low limit of detection of 0.108 fM and a wide concentration detection range of 10 −8 −10 −15 M) for broad-spectrum tumor marker microRNA-155. Also, the 1@PNMPy-based ECBS has a good specific recognition performance for microRNA-155 in a variety of interfering media. The research not only contributes to a deeper understanding of the synthetic chemistry of multicomponent polyoxometalate (POM)-based materials but also can further expand innovative applications of multicomponent POM-based materials in electrochemical detection and electrochemical devices.

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Dual amplified electrochemical sensing coupling of ternary hybridization-based exosomal microRNA recognition and perchlorate-assisted electrocatalytic cycle

Cited by 3 publications

References 51 publications

DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ

DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ

A disposable electrochemical magnetic immunosensor for the rapid and sensitive detection of 5-formylcytosine and 5-carboxylcytosine in DNA

Electrochemical Biosensor Based on Dual-Ligand Functionalized Lanthanide-Encapsulated Polyoxometalate Conductive Polymer Film for Detecting Broad-Spectrum Tumor Marker MicroRNA-155

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