Dual and asynchronous deposition of laminin chains at the epithelial-mesenchymal interface in the gut

Simo, Pauline; Bouziges, Françoise; Lissitzky, Jean‐Claude; Sorokin, Lydia; Kédinger, Michèle; Simon‐Assmann, Patricia

doi:10.1016/0016-5085(92)90303-g

Cited by 67 publications

(39 citation statements)

References 39 publications

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“…In some infants with tufting enteropathy, abnormal laminin and heparin sulfate proteoglycan deposition on basement membrane has been reported, i.e., faint and irregular laminin deposition at the epithelial-lamina propria interface, while heparin sulfate proteoglycan appeared widened and lamellar (5). Basement membrane molecules are involved in epithelial-mesenchymal cell interactions, which are instrumental in intestinal development and differentiation (33,34). The patient with tufting enteropathy reported here is the first case in Korea.…”

Section: Discussionmentioning

confidence: 79%

Protracted Diarrhea: Results of the Five-year Survey in a Tertiary Hospital in Korea

2001

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The syndrome of protracted diarrhea (PD) includes several diseases with diverse etiologies. This study was conducted to characterize the spectrum of causes, clinical manifestations, and the outcomes of PD. A retrospective analysis of the clinical and pathological findings was performed on 25 patients with diarrhea starting within the first 2 yr of life and a requirement of parenteral nutrition (PN). According to the intestinal histopathology, patients were classified into four groups: immune enteropathy (12 cases), lymphangiectasia (6 cases), epithelial dysplasia (5 cases), and unclassified (2 cases). All patients with epithelial dysplasia had earlier onset of diarrhea and longer duration of PN than those in the other groups. Three patients (12%) had an evidence of a familial condition. Five patients (three with microvillous inclusion disease and two with immune enteropathy) died. Sixteen patients recovered, and three (two with primary lymphangiectasia and one with microvillous inclusion disease) still had diarrhea. One patient underwent intestinal transplantation for tufting enteropathy. In conclusion, infants with PD should be referred to specialized centers where advanced diagnostic and therapeutic facilities are available, because histological analysis is critical for the diagnosis of PD, and PN or intestinal transplantation is the only therapeutic option in a subset of cases.

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Section: Discussionmentioning

confidence: 79%

Protracted Diarrhea: Results of the Five-year Survey in a Tertiary Hospital in Korea

2001

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“…Indeed, the concept that laminin ␣1 chain is essential for epithelial cells at early stages of development, whereas laminin ␣5 predominates in more mature epithelial cells, has emerged from observations made in various organs. 48 -51 During intestinal morphogenesis, both laminin ␣1 and ␣5 chains are detectable at the epithelial/mesenchymal interface at the earliest stages studied, the ␣1 chain being produced exclusively by the epithelial cells and the ␣5 chain by both epithelial and mesenchymal cells, 52,53 but the expression and basal segregation of the ␤4 integrin subunit are only obvious in a second step. 54 Similarly, the expression and basal segregation of the ␤4 integrin subunit is induced in cocultures of Caco2 cells on mesenchymal cells, conditions which allow the formation of an organized basement membrane.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

et al. 2001

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Laminins represent a growing family of glycoproteins constituting the basement membrane. They are known to direct many biological processes. With respect to carcinogenesis, laminins play an important role in cell adhesion, mitogenesis, differentiation and even metastasis. To further study the biological significance of laminin-1 (composed of ␣1, ␤1 and ␥1 chains) in intestinal cell differentiation or tumorigenesis, an ␣1-laminin expression vector was introduced into the HT29 colonic cancer cells, in which laminin ␣1 chain is not expressed. Upon transfection of the ␣1 chain, the ␣1␤1␥1 trimer was found secreted in the media along with free Key words: laminins; basement membrane; colonic cancer cells; tumor growth; differentiationIt is well established that in multicellular organisms, extracellular matrix molecules control various physiologic activities such as cell growth, migration, apoptosis and differentiation. The extracellular network includes the interstitial matrix and basement membranes. The basement membranes that separate the connective tissue from epithelia, muscle fibers, blood vessels and nerves are formed by a complex set of collagens and of noncollagenous glycoproteins such as laminins, nidogen and of proteoglycans, which have a unique composition in each organ. Laminins are multifunctional molecules forming a family of proteins that display organ, site and developmental specificity. 1,2 The first characterized laminin isoform, laminin-1 (composed of ␣1, ␤1 and ␥1 chains), is a crosslike-shaped molecule assembled into a triplestranded coiled-coil structure designated as the long arm. 3 As for the other extracellular matrix molecules, laminin-1 is made up of specific structural domains that allow self-assembly, permit interaction with other basement membrane molecules and encode information for the cells via specific transmembrane receptors, mostly of the integrin family. 4 Recent studies performed on various organs during development show that laminin-1 has a rather limited expression. 5 Despite such restricted expression, crucial roles for laminin-1 in organ development and in cell differentiation have been demonstrated by cell-coating experiments, blocking antibody and antisense strategies. Several major functions have been assigned to the constitutive ␣1 chain of laminin-1, among which are the polarization of developing kidney tubules, 6,7 lung alveolar formation, 8 development of submandibular glands 9 and polarization and functional differentiation of mammary and intestinal cells. 10,11 Conversely, alterations in cell-matrix and cell-cell interactions have been shown in a number of pathologic situations, such as inflammatory processes, wound healing and malignant transformation. In colorectal cancer, perturbations in the production, deposition and degradation of matrix molecules, among which are laminins, have been observed. [12][13][14][15][16] The role of laminin-1 in tumor growth and metastasis has been evaluated in several cell systems. In particular, it was shown that coinjection of puri...

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“…The molecules which constitute the basement membrane in the intestine are of dual origin: some of them are produced by the epithelial compartment, others by the mesenchymal cells (Simon-Assmann et al, 1988) and others by both. As an example, the constituent chains of laminin are deposited in the basement membrane as follows: B chains are produced by both cell compartments; A chains are exclusively of epithelial origin during the early morphogenetic period but are additionally produced by the mesenchymal cells when the proliferative cell compartment is segregated (Simo et al, 1992a). …”

Section: Introductionmentioning

confidence: 99%

Differential expression of laminin isoforms and α6‐β4 integrin subunits in the developing human and mouse intestine

Simon‐Assmann¹,

Duclos²,

Orian-Rousseau³

et al. 1994

Developmental Dynamics

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109

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The intestinal tissue is characterized by important morphogenetic movements during development as well as by a continuous dynamic crypt to villus epithelial cell migration leading to differentiation of specialized cells. In this study, we have examined the spatio-temporal distribution of laminin A and M chains as well as of a6 and p4 integrin subunits in adult and developing human and mouse intestine by indirect immunofluorescence. Selective expression of the constituent polypeptides of laminin isoforms (A and M chains) was demonstrated. In the mature human intestine, A and M chains were found to be complementary, the M chain being restricted to the base of crypts and the A chain lining the villus basement membrane. In the developing human intestine, M chain expression was delayed as compared to that of A chain; as soon as the M chain was visualized, it exhibited the typical localization in the crypt basement membrane. A somewhat different situation was found in the adult mouse intestine, since both M and A chains were found in the crypts. During mouse intestinal development the delayed expression of the M chain as compared to that of the A chain was also obvious. The absence of M chain expression in mutant dy mouse did not impair intestinal morphogenesis nor cell differentiation. The expression of a6 and p4 subunits was not coordinated. In both species the a6 expression preceded that of p4. Furthermore, while p4 staining in adult mouse intestine was detected at the basal surface of all cells lining the cryptvillus, that of a6 was mainly confined to the crypt cell compartment. An overall similarity of location between a6 integrin subunit and laminin A chain at the epithelial/stromal interface was noted. These data indicate that the spatial and temporal distribution of laminin variants in the developing intestine may be characteristic for each species and that interactions of laminin variants with particular receptors may be important for induction and/or maintenance of differentiated cells.

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Dual and asynchronous deposition of laminin chains at the epithelial-mesenchymal interface in the gut

Cited by 67 publications

References 39 publications

Protracted Diarrhea: Results of the Five-year Survey in a Tertiary Hospital in Korea

Protracted Diarrhea: Results of the Five-year Survey in a Tertiary Hospital in Korea

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

Differential expression of laminin isoforms and α6‐β4 integrin subunits in the developing human and mouse intestine

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