Dual Aptamer-DNAzyme based colorimetric assay for the detection of AFB1 from food and environmental samples

Setlem, Keerthana; Mondal, Bhairab; Shylaja, R.; Parida, Manmohan

doi:10.1016/j.ab.2020.113874

Cited by 44 publications

(10 citation statements)

References 24 publications

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“…Aflatoxin B (AfB) is especially carcinogenic, making it a focus in research. Wang et al and Setlem et al both detailed strategies that involved using peroxidase-mimicking DNAzymes for the colorimetric detection of AfB. , While Wang et al used a split DNAzyme design and competitive aptamer binding for the detection of the target, Setlem used a closed loop of blocking linker to inhibit DNAzyme binding to an AfB aptamer. In this case, AfB binding to the aptamer led to the loop opening and subsequent DNAzyme attachment for colorimetric detection.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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“…DNAzymes with nuclease and biosensing amplification abilities to catalyze a number of reactions have attracted much interest as catalytic labels in CM assays due to their advantages of stable nature, easy preparation, and modification, as well as good thermal and chemical stability. Setlem et al (2020) devised a CM aptasensor consisting of aptamer-based one-step separation of AFB 1 by biotinylated aptamer conjugated to streptavidin-coated MBs, and subsequent detection of AFB 1 by HRP-DNAzyme labeled AFLA53 through generation of CM signal in the presence of hemin, H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB). The absorbance of blue color generated by the catalytic reaction was linearly correlated with AFB 1 concentration with an LOD as low as 22.6 ppb, exhibiting high stability, specificity, and sensitivity for AFB 1 quantification in sorghum and natural samples.…”

Section: Aptasensorsmentioning

confidence: 99%

“…Setlem et al. (2020) devised a CM aptasensor consisting of aptamer‐based one‐step separation of AFB 1 by biotinylated aptamer conjugated to streptavidin‐coated MBs, and subsequent detection of AFB 1 by HRP‐DNAzyme labeled AFLA53 through generation of CM signal in the presence of hemin, H 2 O 2 and 3,3′,5,5′‐tetramethylbenzidine (TMB). The absorbance of blue color generated by the catalytic reaction was linearly correlated with AFB 1 concentration with an LOD as low as 22.6 ppb, exhibiting high stability, specificity, and sensitivity for AFB 1 quantification in sorghum and natural samples.…”

Section: Typical Aptasensors For Mycotoxin Detectionmentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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“…Detection with the aid of magnetic beads-based separation has emerged as a rapid, simple, reliable, and efficient alternative to conventional immobilization methods. In this regard, a colorimetric aptasensor based on apta-magnetic separation assisted with DNAzyme was developed for AFB1 detection [17]. The procedure consisted of one-step separation of AFB1 by biotinylated aptamer conjugated to streptavidin magnetic beads which was followed by the addition of DNAzyme modified aptamer in the presence hemin and TMB/H 2 O 2 to produce a colorimetric signal.…”

Section: Enzymes-based Probesmentioning

confidence: 99%

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Majdinasab

Aissa

Marty

2020

Toxins

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Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.

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Dual Aptamer-DNAzyme based colorimetric assay for the detection of AFB1 from food and environmental samples

Cited by 44 publications

References 24 publications

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

Aptasensors for mycotoxins in foods: Recent advances and future trends

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

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