Dual-Band Stimulated Parametric Emission Microscopy

Abstract

We demonstrate that two distinct images can be simultaneously acquired using the dual-band configuration of stimulated parametric emission (SPE) microscopy, which is based on the four-wave mixing process enhanced by two-photon electronic resonance. The dual-band SPE microscopy utilizes two SPE signals simultaneously generated at different wavelengths. In the experiment, we image stained and unstained biological tissues, and obtain two different images for each sample. Additionally, we propose an axial differential imaging technique to improve the contrast of SPE images, and verify its applicability to biological imaging. #